Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 262-269.doi: 10.3724/SP.J.1006.2009.00262

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity Analysis for Germplasm of Erianthus rockii in China

LIU Xin-Long1,CAI Qing2,*,BI Yan1,LU Xin1,MA Li1,YING Xiong-Mei1   

  1. 1Sugarcane Research institute, Yunnan Academy Academy of Agricultural sciences,Kaiyuan 661600,China;2Biotechnology & Geneic Germplasm institute, Yunnan Academy of agricultural sciences, Kunming 650223,China
  • Received:2008-06-16 Revised:2008-09-04 Online:2009-02-12 Published:2008-12-11
  • Contact: CAI Qing

Abstract:

Erianthus rockii is the most important wild resource in sugarcane germplasm, which mainly distributes in Yunnan Province. To well utilize the germplasm in breeding program, it is necessary to understand its genetic background. In this research, 41 clones from national nursery of sugarcane germ were amplified with 10 informative AFLP primers. In total, 860 bands were detected, 629 of which were polymorphic, 36 of which were specific bands, and rate of polymorphic bands was 0.73. The results of genetic similarity coefficients, UPGMA cluster, and principal component analysis showed that 41 sugarcane clones were divided into three groups at 0.715 of genetic similarity coefficient:Yundian 07/23 was only grouped into group A and should be further identified due to its back hair on the sheath different from others, group B included three sugarcane clones from high altitude area, group C included 37 sugarcane clones, containing of 30 from southwest of Yunnan, and 7 from other region. Groups C was divided into 4 sub-groups at 0.738 of genetic similarity coefficient, and the cluster relation was obviouslyreflected the geographic distribution of the clones.Sub-group C4 was divided into 4 sub-sub-groups at 0.765 of genetic similarity coefficient. Yundian07/9/1 and Yundian99/4 in sub-sub-group C4-3 were the most similar, indicating that two clones were duplicate. Sub-sub-group C4-3 included three branches according to 0.773 of genetic similarity coefficient. Principal component analysis for all clones indicated same result obtained from genetic similarity coefficients analysis. Therefore, complex geographical ecological environment is important factor to the genetic diversity and geographical distribution of E. rockii clones.

Key words: Erianthus rockii, AFLP marker, Genetic diversity

[1] Editorial Board of the Flora of China (中国植物志编辑部委员会). Flora of China, Vol. 9 (中国植物志·第9卷). Beijing: Sci-ence Press, 2002. pp 48-49(in Chinese)
[2] Chen R-K(陈如凯). Theory and Practice in Modern Sugarcane Breeding (现代甘蔗育种的理论与实践). Beijing: China Agri-culture Press, 2003. pp 2-7(in Chinese)
[3] Wang L-P(王丽萍), Cai Q(蔡青), Lu X(陆鑫), Ma L(马丽), Liu X-L(刘新龙), Li W-F(李文凤), Xia H-M(夏红明). Study of wild species Erianthus rockii germplasm innovation and use. Sugar Crops China (中国糖料), 2008, (2): 8-11(in Chinese with Eng-lish abstract)
[4] Xiao F-H(肖凤回). Comparison between E. arundinaceus and some species under Saccharum and Erianthus on the plant mor-phology and isozyme. Sugarcane (甘蔗), 1994, 1(1): 22-27(in Chinese with English abstract)
[5] Cai Q(蔡青), Fan Y-H(范源洪), Aitken K, Piperidis G, Mcintyre C L, Jackson P. Assessment of the phylogenetic relationships within the “Saccharum complex” using AFLP markers. Acta Agron Sin (作物学报), 2005, 31(5): 551-559(in Chinese with English abstract)
[6] Cai Q, Aitken K, Fan Y H, Piperidis G, Jackson P, Mcintyre C L. A preliminary assessment of the genetic relationship between Erian-thus rockii and the ‘‘Saccharum complex’’ using micro-satellite (SSR) and AFLP markers. Plant Sci, 2005, 169: 976-984
[7] Guo X-M(郭雄明), Xue X(薛霞), Chen H(陈华). Progress in re-search on amplified fragment length polymorphism (AFLP). China J Comparative Med (中国比较医学杂志), 2006, 16(6): 369-372(in Chinese with English abstract)
[8] Doyle J J, Doyle J I. Isolation of plant DNA from fresh tissue. Focus, 1990, 12: 149-151
[9] Vos P, Hogers R, Bleeker M, Reijans M, Van Der Lee T, Hornes M. AFLP: A new concept for DNA fingerprinting. Nucl Acids Res, 1995, 23: 4407-4414
[10] Powell W, Morgante M, Andre C. The comparison RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed, 1996, 2: 225-238
[11] Nei M, Kumar S. Molecular Evolution and Phylogenetics. New York: Oxford University Press, 2000. pp 87-88
[12] Xu A-X(徐爱遐), Ma C-Z(马朝芝), Xiao E-S(肖恩时), Quan J-C(权景春), Ma C-Z(马长珍), Tian G-W(田广文), Tu J-X(涂金星), Fu T-D(傅廷栋), Zhang G-S(张改生). Genetic diversity of Brassica juncea from western China. Acta Agron Sin (作物学报), 2008, 34(5): 754-763(in Chinese with English abstract)
[13] Yan L(闫龙), Guan J-P(关建平), Zong X-X(宗绪晓). Genetic diversity analysis of pigeonpea germplasm resources by AFLP. Acta Agron Sin (作物学报), 2007, 33(5): 790-798(in Chinese with English abstract)
[14] Liu Y(刘勇), Sun Z-H(孙中海), Liu D-C(刘德春), Wu B(吴波), Tao J-J(陶建军). Assessment of the genetic diversity of pummelo germplasms using AFLP and SSR markers. Sci Agric Sin (中国农业科学), 2005, 38(11): 2308-2315(in Chinese with English ab-stract)
[15] Zhuang N-S(庄南生), Zheng C-M(郑成木), Huang D-Y(黄东益), Tang Y-Q(唐燕琼), Gao H-Q(高和琼). AFLP analysis for sugar-cane Germplasms. Acta Agron Sin (作物学报), 2005, 31(4): 444-450(in Chinese with English abstract)
[16] Aitken K S, Li J C, Jackson P, Piperidis G, Mcintyre C L. AFLP analysis of genetic diversity within Saccharum officinarum and comparison with sugarcane cultivars. Aust J Agric Res, 2006, 57: 1167-1184
[17] Selvi A, Nair N V, Noyer J L, Singh N K, Balasundaram N, Bansal K C, Koundal K R, Mohapatra T. AFLP analysis of the phenetic organization and genetic diversity in the sugarcane com-plex, Saccharum and Erianthus. Genet Resour Crop Evol, 2006, 53: 831-842
[18] Liu X-L(刘新龙), Cai Q(蔡青), Wang L-P(王丽萍), Ma L(马丽), Lu X(陆鑫), Ying X-M(应雄美), Mao J(毛钧). The advance of sugarcane germplasm resource in genetic and crossing diversity utilization. Sugar Crops China (中国糖料), 2007, (1): 46-49(in Chinese with English abstract)
[1] XIAO Ying-Ni, YU Yong-Tao, XIE Li-Hua, QI Xi-Tao, LI Chun-Yan, WEN Tian-Xiang, LI Gao-Ke, HU Jian-Guang. Genetic diversity analysis of Chinese fresh corn hybrids using SNP Chips [J]. Acta Agronomica Sinica, 2022, 48(6): 1301-1311.
[2] WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[3] LIU Shao-Rong, YANG Yang, TIAN Hong-Li, YI Hong-Mei, WANG Lu, KANG Ding-Ming, FANG Ya-Ming, REN Jie, JIANG Bin, GE Jian-Rong, CHENG Guang-Lei, WANG Feng-Ge. Genetic diversity analysis of silage corn varieties based on agronomic and quality traits and SSR markers [J]. Acta Agronomica Sinica, 2021, 47(12): 2362-2370.
[4] SUN Qian, ZOU Mei-Ling, ZHANG Chen-Ji, JIANG Si-Rong, Eder Jorge de Oliveira, ZHANG Sheng-Kui, XIA Zhi-Qiang, WANG Wen-Quan, LI You-Zhi. Genetic diversity and population structure analysis by SNP and InDel markers of cassava in Brazil [J]. Acta Agronomica Sinica, 2021, 47(1): 42-49.
[5] Meng-Liang ZHAO,Li-Hui WANG,Yan-Jing REN,Xue-Mei SUN,Zhi-Qiang HOU,Shi-Peng YANG,Li LI,Qi-Wen ZHONG. Genetic diversity of phenotypic traits in 257 Jerusalem artichoke accessions [J]. Acta Agronomica Sinica, 2020, 46(5): 712-724.
[6] Hong-Yan ZHANG,Tao YANG,Rong LIU,Fang JIN,Li-Ke ZHANG,Hai-Tian YU,Jin-Guo HU,Feng YANG,Dong WANG,Yu-Hua HE,Xu-Xiao ZONG. Assessment of genetic diversity by using EST-SSR markers in Lupinus [J]. Acta Agronomica Sinica, 2020, 46(3): 330-340.
[7] MA Yan-Ming, FENG Zhi-Yu, WANG Wei, ZHANG Sheng-Jun, GUO Ying, NI Zhong-Fu, LIU Jie. Genetic diversity analysis of winter wheat landraces and modern bred varieties in Xinjiang based on agronomic traits [J]. Acta Agronomica Sinica, 2020, 46(12): 1997-2007.
[8] MA Yan-Ming, LOU Hong-Yao, CHEN Zhao-Yan, XIAO Jing, XU Lin, NI Zhong-Fu, LIU Jie. Genetic diversity assessment of winter wheat landraces and cultivars in Xinjiang via SNP array analysis [J]. Acta Agronomica Sinica, 2020, 46(10): 1539-1556.
[9] LIU Yi-Ke,ZHU Zhan-Wang,CHEN Ling,ZOU Juan,TONG Han-Wen,ZHU Guang,HE Wei-Jie,ZHANG Yu-Qing,GAO Chun-Bao. Revealing the genetic diversity of wheat varieties (lines) in China based on SNP markers [J]. Acta Agronomica Sinica, 2020, 46(02): 307-314.
[10] YE Wei-Jun,CHEN Sheng-Nan,YANG Yong,ZHANG Li-Ya,TIAN Dong-Feng,ZHANG Lei,ZHOU Bin. Development of SSR markers and genetic diversity analysis in mung bean [J]. Acta Agronomica Sinica, 2019, 45(8): 1176-1188.
[11] Mi WU,Nian WANG,Chao SHEN,Cong HUANG,Tian-Wang WEN,Zhong-Xu LIN. Development and evaluation of InDel markers in cotton based on whole-genome re-sequencing data [J]. Acta Agronomica Sinica, 2019, 45(2): 196-203.
[12] Yuan LU,Wei-Da AI,Qing HAN,Yi-Fa WANG,Hong-Yang LI,Yu-Ji QU,Biao SHI,Xue-Fang SHEN. Genetic diversity and population structure analysis by SSR markers in waxy maize [J]. Acta Agronomica Sinica, 2019, 45(2): 214-224.
[13] XUE Yan-Tao,LU Ping,SHI Meng-Sha,SUN Hao-Yue,LIU Min-Xuan,WANG Rui-Yun. Genetic diversity and population genetic structure of broomcorn millet accessions in Xinjiang and Gansu [J]. Acta Agronomica Sinica, 2019, 45(10): 1511-1521.
[14] Hong LIU,Zhen-Jiang XU,De-Hua RAO,Qing LU,Shao-Xiong LI,Hai-Yan LIU, ,Xuan-Qiang LIANG,Yan-Bin HONG. Genetic diversity analysis and distinctness identification of peanut cultivars based on morphological traits and SSR markers [J]. Acta Agronomica Sinica, 2019, 45(1): 26-36.
[15] Dong-Mei BAI,Yun-Yun XUE,Jiao-Jiao ZHAO,Li HUANG,Yue-Xia TIAN,Bao-Quan QUAN,Hui-Fang JIANG. Identification of Cold-tolerance During Germination Stage and Genetic Diversity of SSR Markers in Peanut Landraces of Shanxi Province [J]. Acta Agronomica Sinica, 2018, 44(10): 1459-1467.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!