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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 662-671.doi: 10.3724/SP.J.1006.2009.00662

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Fertility Alternation of Thermo-photo-sensitive Genic Male Sterile Wheat Line C412S and Expression of Fertility Related APRT Gene

ZHANG Jian-Kui1;DONG Jing12;ZONG Xue-Feng1;YU Guo-Dong3;DAI Xiu-Mei1;RUAN Ren-Wu1   

  1. 1College of Agronomy and Biotechnology/Key Laboratory of Biotechnology and Crop Ouality Improvement, Ministry of Agriculture, Southwest University,Chongqing 400716,China;2 Hubei Academy of Agricultural Sciences,Wuhan 430064,China;3 Chongqing Academy of Agricultural Sciences, Chongqing 4012160,China
  • Received:2008-07-16 Revised:2008-10-05 Online:2009-04-12 Published:2009-01-15

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Abstract:

C412S is a novel type of thermo-photo-sensitive genic male sterile (TGMS) wheat (Triticum aestivum L.) line, which is derived from the cross between TGMS line C49S and normal wheat line C412. To understand the fertility alternation of C412S, the courses of pollen mother cell (PMC) meiosis and microsporogenesis were observed using smear and squash microscopic techniques. Moreover, the expression characteristics of adenine phosphoribosyl-transferase gene (APRT) in young spikes were detected under sterile and fertile conditions using quantitative RT-PCR. When adjusting the environmental temperature and photoperiod at the formation and development stages of male sexual cell through series seeding dates, C412S showed continuous changes of fertility from complete sterility to high sterility, semi-sterility, lowe sterility, and normal fertility. C412S in normal PMC meiosis, however, showed a peak pollen abortion at later mononucleate stage of microsporogenesis, which mostly belonged to spherical abortion type. From PMC formation stage to mature pollen stage, C412S was sensitive in fertility, especially from PMC formation stage to PMC meiosis stage. Compared with C412 (control), C412S only had several base variations in a few loci of APRT1 gene, and the deducted amino acid sequences of C412S and C412 were completely identical. The RT-PCR analysis showed that the transcription level of APRT gene in the young spikes of sterile C412S plants was lower than that of fertile C412S plants from PMC formation stage to mono-nucleate microspore stage. Therefore, the transcription level of APRT gene in young spikes is probably related to the fertility alternation in TGMS wheat.

Key words: Wheat(Triticum aestivum L.), Thermo-photo-sensitive genic male sterility(TGMS), Fertility alternation APRT Gene

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