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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (9): 1749-1754.doi: 10.3724/SP.J.1006.2009.01749

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Cloning and Expression of Calcium-Dependent Protein Kinase Genesis TaCPK1A and TaCPK10 in Response to Deficient-Pi in Wheat

LU Wen-Jing2,LI Rui-Juan2,LI Xiao-Juan2,GUO Cheng-Jin1,GU Jun-Tao2,XIAO Kai1*   

  1. 1 College of Agronomy, Agricultural University of Hebei, Baoding 071001, China; 2 College of Life Science, Agricultural University of Hebei, Baoding 071001, China
  • Received:2009-02-27 Revised:2009-04-30 Online:2009-09-12 Published:2009-07-04
  • Contact: XIAO Kai, E-mail: xiaokai@hebau.edu.cn


Two expressed sequence tags (ESTs) of calcium-dependent protein kinase (CDPK) genes responding to deficient-Pi were identified from wheat (Triticum aestivum L.) cultivar Shixin 828 by subtractive suppression cDNA library and cDNA-AFLP approaches. The genes, TaCPK1A and TaCPK10, contained the conserved domains of plant CDPK, were 2 129 bp and 1 696 bp in full length open reading frames of 1599 bp and 1 281 bp, encoding 532 and 426 amino acids,respectively. Phylogenetic analysis implied different ancestors of TaCPK1A and TaCPK10 because of their low identity of sequence. Under low-Pi condition, the expression level of TaCPK1A in roots was strongly inducible and reached the highest at 24 h after treatment, but that in leaves was induced in 1 h of treatment and maintained stably afterwards. The expression of TaCPK10 in roots and leaves both showed a pattern of low–high–low with the peak at 1 h of treatment,and then decreased to the level before treatment. No responses of TaCPK1A and TaCPK10 were observed to low-N and low-K stresses. Therefore, it is suggested that CDPK playsan important role in mediating phosphate-starvation signal in wheat. There are at least two phosphorylation reaction pathways for transduction of low-Pi signal, in which CDPKs are involved.

Key words: Wheat(Triticum aestivum L.), Calcium-dependent protein kinase, Cloning, Low-Pi stress, Gene expression

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