Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 952-957.doi: 10.3724/SP.J.1006.2009.00952

• RESEARCH NOTES • Previous Articles     Next Articles

Subcellular Localization and Genetic Polymorphism of Isoform of Starch Branching Enzyme(SBE IIb)in Wheat Grain

ZHAO Fa-Mao1,CAI Rui-Guo2,BI Jian-Jie3,XIAO Jun1,WANG Xian-Ze3*   

  1. 1Department of Biology,Taishan College,Tai'an 271021,China;2Agronomy Department,Hebei Normal University of Science and Technology,Changli 066600,China;3State Key Laboratory of Crop biology,Shandong Agricultural University,Tai'an 271018,China
  • Received:2008-09-07 Revised:2009-02-17 Online:2009-05-12 Published:2009-03-23
  • Contact: WANG Xian-Ze,E-mail:xzwang@sdau.edu.cn


To elucidate subcellular localization and genetic polymorphism of SBE IIb, the isoform of starch branching enzyme (SBE), in wheat grain and make insight into molecular mechanism of amylopectin biosynthesis. We identified the differences of SBE IIb among 70 wheat varieties using SDS polyacrylamide gel electrophoresis (SDS-PAGE), recovered SBE IIb from polyacrylamide gel and determined its enzymatic activities. The expression levels of SBE IIb were examined during grain-filling period. The result indicated that starch granule-bound protein SGP-2, was granule-associated SBE IIb with molecular weight of 85 kD. It possessed SBE activity when SDS was removed by acetone precipitation, which reached the highest activity at the 21th day after anthesis. SBE IIb expressed at early developing stage of wheat grain and expression quantity exhibited differences in different developing periods. Notably, the expression profile of SBE IIb showed no differences among 70 varieties.

Key words: Wheat(Triticum aestivum L.), Starch granule-bound proteins(SGP), SBE IIb, Genetic polymorphism

[1] Baga M, Glaze S, Mallard C S, Chibbar R. A starch branching enzyme gene in wheat produces alternatively spliced transcripts. Plant Mol Biol, 1999, 40: 1019–1030

[2] Yao D-N(姚大年), Li B-Y(李保云), Zhu J-B(朱金宝), Liang R-Q(梁荣奇), Liu G-T(刘广田). Study on main starch properties and predictive indexes of noodle quality in common wheat. Sci Agric Sin (中国农业科学), 1999, 32(6): 84–88(in Chinese with English abstract)

[3] McCormick K M, Panozzo J F, Hong S H. A swelling power test for selecting potential noodle quality wheats. Aust J Agric Res, 1991, 42: 317–323

[4] Mu-Forster C, Huang R, Powers J R, Harriman R W, Knight M, Singletary G W, Keeling P L, Wasserman B P. Physical association of starch biosynthetic enzymes with starch granules of maize endosperm. Plant Physiol, 1996, 111: 821–829

[5] Satoh H, Nishi A, Yamashita K, Takemoto Y, Tanaka Y, Hosaka Y, Sakurai A, Fujita N, Nakamura Y. Starch-branching enzyme I-deficient mutation specifically affects the structure and properties of starch in rice endosperm. Plant Physiol, 2003, 133: 1111–1121

[6] Guan H P, Preiss J. Differentiation of the properties of the branching isozymes from maize (Zea mays). Plant Physiol, 1993, 102: 1269–1273

[7] Morell M K, Blennow A, Hashemi B K, Samuel M S. Differential expression and properties of starch branching nzyme isoforms in developing wheat endosperm. Plant Physiol, 1997, 113: 201–208

[8] Regina A, Kosar H B, Li Z, Pedler A, Mukai Y, Yamamoto M, Gale K, Sharp P J, Morell M K, Rahman S. Starch branching enzyme IIb in wheat is expressed at low levels in the endosperm compared to other cereals and encoded at a non-syntenic locus. Planta, 2005, 222: 899–909

[9] Stinard P S, Robertson D S, Schnable P S. Genetic isolation, cloning and analysis of mutator-induced, dominant antimorph of maize amylose extenter 1 locus. Plant Cell, 1993, 5: 1555–1566

[10] Nishi A, Nakamura Y, Tanaka N, Satoh H. Biochemical and genetic effects of amylase-extender mutation in rice endosperm. Plant Physiol, 2001, 127: 459–472

[11] Tetlow I J, Wait R, Lu Z X, Akkasaeng R, Bowsher C G, Esposito S, Hashemi B K, Morell M K, Emes M J. Protein phosphorylation in amyloplasts regulates starch branching enzyme activity and protein-protein interactions. Plant Cell, 2004, 16: 694–708

[12] Nagamine T, Yoshida H, Komae K. Varietal differences and chromosome locations of multiple isoforms of starch branching enzyme in wheat endosperm. Phytochemistry, 1997, 46: 23–26

[13] Zhao F-M(赵法茂), Bi J-J(毕建杰), Li T-J(李天骄), Pang X-Y(逄孝云), Wang X-Z(王宪泽). Relationship between isozyme genotypes and activities of starch branching enzyme in wheat grain. Acta Agron Sin (作物学报), 2007, 33(11): 1850–1855 (in Chinese with English abstract)

[14] Denyer K, Sidebottom C, Hylton C H, Smith A M. Soluble isoforms of starch synthase and starch-branching enzyme also occur within starch granules in developing pea embryos. Plant J, 1993, 4: 191–198

[15] Denyer K, Hylton C M, Jenner C F, Smith A M. Identification of multiple isoforms of soluble and granule-bound starch synthase in developing wheat endosperm. Planta, 1995, 196: 256–265

[16] Rahman S, Hashemi B K, Samuel M S, Hill A, Abbott D C, Skerritt J H, Preiss J, Appels R, Morell M K. The major proteins of wheat endosperm starch granules. Aust J Plant Physiol, 1995, 22: 793–803

[17] Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227: 680–685

[18] Yamamori M, Endo T R. Variation of starch granule proteins and chromosome mapping of their coding genes in common wheat. Theor Appl Genet, 1996, 93: 275–281

[19] Hager D A, Burgess R R. Elution of proteins from SDS-polyacrylamide gels, removal of SDS, and renaturation of enzymatic activity: Results with sigma subunit of E. coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes. Anal Biochem, 1980, 109: 76–86

[20] Dynan W S, Jendrisak JJ, Hager D A, Burgess R D. Purification and characterization of wheat germ DNA topoisomerase I (nicking-closing enzyme). J Biol Chem, 1981, 256: 5860–5865

[21] Li T-G(李太贵), Shen B(沈波), Chen N(陈能), Luo Y-K(罗玉坤). Effect of Q-enzymeon the chalkiness formation of rice grain. Acta Agron Sin (作物学报), 1997, 23(3): 338–344 (in Chinese with English abstract)

[22] Takaoka M, Watanabe S, Sassa H, Yamamori M, Nakamura T, Sasakuma T, Hirano H. Structural characterization of high molecular weight starch granule-bound proteins in wheat (Triticum aestivum L.). J Agric Food Chem, 1997, 45: 2929–2934

[23] Yamamori M, Fujita S, Hayakawa K, Matsuki J, Yasui T. Genetic elimination of a starch granule protein, SGP-1, of wheat generates an altered starch with apparent high amylose. Theor Appl Genet, 2000, 101: 21–29

[24] Baba T, Nishiara M, Mizuno K, Kawasaki T, Shimada H, Kobayashi E, Ohnishi S, Tanaka K, Arai Y. Identification, cDNA cloning, and gene expression of soluble starch synthase in rice (Oryza sativa L.) immature seeds. Plant Physiol, 1993, 103: 565–573

[25] Commuri P D, Keeling P G. Chain-length specificities of maize starch synthase I enzyme: studies of glucan affinity and catalytic properties. Plant J, 2001, 25: 475–486

[26] Regina A, Bird D, Topping D, Bowden S, Freeman J, Barsby T, Hashemi B K, Li Z, Rahman S, Morell M. High amylose wheat generated by RNA-interference improves indices of large bowel health in rats. Proc Natl Acad Sci USA, 2006, 103: 3546–3551
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