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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 946-951.doi: 10.3724/SP.J.1006.2009.00946

• RESEARCH NOTES • Previous Articles     Next Articles

Effects of Endophytic Fungus on Sugar Content and Key Enzymes Activity in Nitrogen and Sugar Metabolism of Sugar Beet(Beta vulgaris L.)

SHI Ying-Wu12,LOU Kai2*,LI Chun1   

  1. 1Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan/Agriculture College of Shihezi University,Shihezi 832003,china;2Institute of Microbiology,Xinjiang Academy of Agriculture Science,Urumqi 830091,China
  • Received:2008-11-13 Revised:2009-02-17 Online:2009-05-12 Published:2009-03-23
  • Contact: LOU Kai,E-mail:loukai02@mail.tsinghua.edu.cn;Tel:0991-4521590


We investigated the agronomic traits and determined the activity of key enzymes in sugar and nitrogen metabolisms in beet leaf and root with treatments of by using Plectosphaerella cucumerina F11 solution seed soaking, leaf spraying and root watering. The results showed that the strain F11 obviously promoted growth of beet seedlings, the effect with the better in root watering treatment than in others. the leaf fresh weight,content of chlorophyll, fresh weight per root, sugar content and sugar yield of beet increased significantly (P<0.05) by 66.67%, 47.42%, 6.96%, 17.46%, and 25.63%, respectively; F11 enhanced evidently the activities of key enzymes in sugar and nitrogen metabolisms of beet during whole growth period. The changes of activities of nitrate reductase (NR) and glutamina synthetase (GS) presented the curves of M-shape and parabola shape, respectively. The changes of activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) in beet displayed the single peak curve. The synthesis activity was much higher than the decomposition activity for SS in roots of beet at late growth stage. The activity of SPS was higher at early growth stage than at late growth stage, and the activity of SPS was the highest during phyllome formation period. It was concluded that the strain F11 not only enhanced activities of key enzymes in sugar and nitrogen metabolisms of beet, but also promoted the growth, yield and sugar accumulation of beet.

Key words: Sugar beet, Endophytic fungus F11, Key enzymes of nitrogen and sugar metabolisms

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