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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 445-451.doi: 10.3724/SP.J.1006.2009.00445

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Influence of Inhibitors of Nucleic Acid Synthesis and Protein Synthesis on Glutamine Synthetase Gene Expression Induced by Nitrogen in Sugar Beet (Beta vulgaris L.)

CHEN Sheng-Yong;HOU Jing**;LI Cai-Feng;MA Feng-Ming;YIN Chun-Jie; HUANG Zhao-Feng   

  1. College of Agriculture, Northeast Agricultural University, Harbin 150030,China
  • Received:2008-06-01 Revised:2008-10-21 Online:2009-03-12 Published:2009-01-16
  • Contact: LI Cai-Feng

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Abstract:

Glutamine synthetase (GS, EC6.3.1.2) families are the key enzymes involving in nitrogen assimilation in the higher plants as well as a core elements for nitrogen use efficiency and nitrogen recycle. The objective of this study was to reveal the effect of actinomycin D (AMD) and cycloheximide (CHM) on GS gene expression and its activities induced by nitrogen in sugar beet. GS activity in sugar beet was determined under the treatment of AMD and CHM. Gene transcripts of cytosolic glutamine synthetase (GS1) and plastidic glutamine synthetase (GS2) were detected by semi-quantitative PCR. And e?ciency of GSmRNA synthesis from each sample was estimated by quantitative PCR of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The results showed that the GS activities increased slightly under the low concentration treatment of AMD for 2–6 hours, but decreased with treatment of all concentrations. The transcript level of GS1mRNA and GS2mRNA decreased with the increase of AMD concentration treated for more than 9 h. GS activities decreased fast with the increase of CHM concentrations treated for more than 9 h. There was no significant difference between GS1mRNA and GS2mRNA expressions in the treatments with all CHM concentrations for more than 9 hours.

Key words: Sugar beet(Beta vulgaris L.), Glutamine synthetase, Nitrogen, Actinonycin D, Cycloheximide

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