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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 445-451.doi: 10.3724/SP.J.1006.2009.00445

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Influence of Inhibitors of Nucleic Acid Synthesis and Protein Synthesis on Glutamine Synthetase Gene Expression Induced by Nitrogen in Sugar Beet (Beta vulgaris L.)

CHEN Sheng-Yong;HOU Jing**;LI Cai-Feng;MA Feng-Ming;YIN Chun-Jie; HUANG Zhao-Feng   

  1. College of Agriculture, Northeast Agricultural University, Harbin 150030,China
  • Received:2008-06-01 Revised:2008-10-21 Online:2009-03-12 Published:2009-01-16
  • Contact: LI Cai-Feng

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Abstract:

Glutamine synthetase (GS, EC6.3.1.2) families are the key enzymes involving in nitrogen assimilation in the higher plants as well as a core elements for nitrogen use efficiency and nitrogen recycle. The objective of this study was to reveal the effect of actinomycin D (AMD) and cycloheximide (CHM) on GS gene expression and its activities induced by nitrogen in sugar beet. GS activity in sugar beet was determined under the treatment of AMD and CHM. Gene transcripts of cytosolic glutamine synthetase (GS1) and plastidic glutamine synthetase (GS2) were detected by semi-quantitative PCR. And e?ciency of GSmRNA synthesis from each sample was estimated by quantitative PCR of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The results showed that the GS activities increased slightly under the low concentration treatment of AMD for 2–6 hours, but decreased with treatment of all concentrations. The transcript level of GS1mRNA and GS2mRNA decreased with the increase of AMD concentration treated for more than 9 h. GS activities decreased fast with the increase of CHM concentrations treated for more than 9 h. There was no significant difference between GS1mRNA and GS2mRNA expressions in the treatments with all CHM concentrations for more than 9 hours.

Key words: Sugar beet(Beta vulgaris L.), Glutamine synthetase, Nitrogen, Actinonycin D, Cycloheximide

[1]Migge A, Carrayol E, Hirel B, Becher T W. Leaf-specific overexpression of plastidic glutamine synthetase stimulates the growth of transgenic tobacco seedlings. Planta, 2000, 210: 252–260
[2]Tobin A K, Yamaya T. Cellular compartmentation of ammonium assimilation in rice and barley. J Exp Bot, 2001, 52: 591–604
[3]Weber A, Flugge U I. Interaction of cytosolic and plastidic nitrogen metabolism in plants. J Exp Bot, 2002, 53: 865–874
[4]Li C-F(李彩凤), Ma F-M(马凤鸣), Zhao Y(赵越). Effects of nitrogen forms on key enzyme activities and related products in sugar and nitrogen metabolism of sugar beet (Beta vulgaris L.). Acta Agron Sin (作物学报), 2003, 29(1): 128–132(in Chinese with English abstract)
[5]Zhao Y(赵越), Ma F-M(马凤鸣), Zhang D-Y(张多英). Study on the absorption kinetics of different nitrogen in sugar beet. J Northeast Agric Univ(东北农业大学学报), 2006, 37(3): 294–298 (in Chinese with English abstract)
[6]Wu X-P(吴小平), Wang P-H(汪沛洪), Zhang H(张惠). Effects of osmotic stress on protease activities of two winter wheat cultivars with different drought resistance. Acta Agric Boreali-Occident Sin (西北农业学报), 1994, 3(1): 49–53(in Chinese with English abstract)
[7]Xue Y-C(薛永常), Chen Y-J(陈永军), Cao M(曹敏). Effect of actinomycin D and cycloheximide on ABA accumulation induced by water stress in winter wheat seedling. J Hebei Norm Univ(Nat Sci)(河北师范大学学报×自然科学版), 1997, 21(3): 317–319(in Chinese with English abstract)
[8]Wang X-K(王学奎), Li H-S(李合生), Liu W-D(刘武定). Preliminary study on mechanism of light regulated glutamine synthetase in wheat leaves. J Huazhong Agric Univ(华中农业大学学报), 2000, 19(2): 102–105(in Chinese with English abstract)
[9]Yin L-P(印莉萍), Chai X-Q(柴晓清), Liu X-L(刘祥林). Influence of chloroplast development and light on the gene expression of glutamine synthetase in wheat leaves. Acta Bot Sin(植物学报), 1994, 36(8): 597–602(in Chinese with English abstract)
[10]Schumidt S, Mobr H. Regulation of the appearance of glutamine synthetase in mustard (Sinap isalba L.) cotyledons by light, nitrate and ammonium. Planta, 1989, 177: 526–534
[11]Hirel B, Cadal P. Glutamine synthetase in rice: A comparative study of the enzymes from roots and leaves. Plant Physiol, 1980, 66: 619–623
[12]Peterman T K, Goodman H M. The glutamine synthetase gene family of Arabidopsis thaliana: Light-regulation and differential expression in leaves, root and seeds. Mol Gen Genet, 1991, 230: 145–154
[13]Chen Y(陈煜), Zhu B-G(朱保葛), Zhang J(张敬). Effects of different nitrogens on activities of nitrate reductase, glutamine synthetase and seed protein contents in soybean vultivars. Soybean Sci(大豆科学), 2004, 23(2): 143–146(in Chinese with English abstract)
[14]Dai T-B(戴廷波), Cao W-X(曹卫星), Sun C-F(孙传范). Effect of enhanced ammonium nutrition on photosynthesis and nitrate reductase and glutamine synthetase activities of winter wheat. Chin J Appl Ecol (应用生态学报), 2003, 14(9): 1529–1532(in Chinese with English abstract)
[15]Mack G. Organ-specific changes in the activity and subunit composition of glutamine synhetase isoforms of barley (Hordeum vulgare L.) after growth on different levels of NH4+. Planta, 1995, 196: 231–238
[16]Zhang C F, Peng S B, Peng X X. Response of glutamine synthetase isoforms to nitrogen sources in rice (Oryza sativa L.) roots. Plant Sci, 1997, 125: 163–170
[17]Lam H M, Coschigano K T, Oliveira I C. The molecular genetics of nitrogen assimilation into amino acids in higher plants. Annu Rev Plant Physiol Plant Mol Biol, 1996, 47: 569–593
[18]Li C-J(李常健), Lin Q-H(林清华), Zhang C-F(张楚富). Effect of NaCl stress on activity and isozymes of glutamine synthetase in rice plants. J Wuhan Univ(Nat Sci Edn)(武汉大学学报×自然科学版), 1999, 45(4): 497–500(in Chinese with English abstract)
[19]Hoshi H, Tanaka Y, Hibino T. Enhanced tolerance to salt stress in transgenic rice that overexpress chloroplast glutamine synthetase. Plant Mol Biol, 2000, 43: 103–111
[20]Miflin B J, Lea P J. Ammonia Assimilation in the Biochemistry of Plants: Amino Acids Their Derivatives. New York: Academic Press, 1980. pp 169–202
[21]Oliveira L C, Coruzzi G M. Carbon and Amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis. Plant Physiol, 1999, 121(1): 301–309
[22]Chen S-Y(陈胜勇), Li C-F(李彩凤), Ma F-M(马凤鸣), Yang D-G(杨德光), Hou J(侯静), Sun S-C(孙世臣), Yin C-J(尹春佳), Huang Z-F(黄兆峰), Zhao L-Y(赵丽影), Chen Y-T(陈业婷), Yue P(越鹏). Expression analysis of glutamine synthetase gene under different nitrogen conditions in sugar beet (Beta vulgaris L.). Crops (作物杂志), 2008, (4): 64–67(in Chinese with English abstract)
[23]Kang S M, Titus J S. Activity pro?les of enzymes involved in glutamine and glutamate metabolism in the apple during autumnal snescence. Physiol Plant, 1980, 50: 291–297
[24]Kang S M, Titus J S. Increased proteolysis of senescing rice leaves in the presence of NaCl and KCl. Plant Physiol, 1989, 91: 1232–1237
[25]Kar M, Feierabend J. Changes in the activities of enzymes involved in amino acid metabolism during the senescence of detached wheat leaves. Physiol Plant, 1984, 62: 39–44
[26]Yuang H, Hou C A. comparative study of two forms of glutamine synthetase from rice leaves. Bot Bull Acad Sin, 1987, 28: 91–108
[27]Han N(韩娜), Ge R-C(葛荣朝), Zhao B-C(赵宝存). Research development of the glutamine synthetase in plants. J Hebei Norm Univ(Nat Sci Edn)(河北师范大学学报×自然科学版), 2004, 28(4): 407–410 (in Chinese with English abstract)
[28]Becker K, Pan D, Whitley C B. Real-time quantitative polymerase chain reaction to assess gene transfer. Human Gene Therapy, 1999, 10: 2559–2566
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