内生真菌对甜菜主要农艺性状及氮糖代谢关键酶活性的影响

doi:10.3724/SP.J.1006.2009.00946

Abstract

Abstract:

We investigated the agronomic traits and determined the activity of key enzymes in sugar and nitrogen metabolisms in beet leaf and root with treatments of by using Plectosphaerella cucumerina F11 solution seed soaking, leaf spraying and root watering. The results showed that the strain F11 obviously promoted growth of beet seedlings, the effect with the better in root watering treatment than in others. the leaf fresh weight,content of chlorophyll, fresh weight per root, sugar content and sugar yield of beet increased significantly (P<0.05) by 66.67%, 47.42%, 6.96%, 17.46%, and 25.63%, respectively; F11 enhanced evidently the activities of key enzymes in sugar and nitrogen metabolisms of beet during whole growth period. The changes of activities of nitrate reductase (NR) and glutamina synthetase (GS) presented the curves of M-shape and parabola shape, respectively. The changes of activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) in beet displayed the single peak curve. The synthesis activity was much higher than the decomposition activity for SS in roots of beet at late growth stage. The activity of SPS was higher at early growth stage than at late growth stage, and the activity of SPS was the highest during phyllome formation period. It was concluded that the strain F11 not only enhanced activities of key enzymes in sugar and nitrogen metabolisms of beet, but also promoted the growth, yield and sugar accumulation of beet.

Key words: Sugar beet, Endophytic fungus F11, Key enzymes of nitrogen and sugar metabolisms

SHI Ying-Wu,LOU Kai,LI Chun. Effects of Endophytic Fungus on Sugar Content and Key Enzymes Activity in Nitrogen and Sugar Metabolism of Sugar Beet(Beta vulgaris L.)[J].Acta Agron Sin, 2009, 35(5): 946-951.

References

[1] Li W-S(李文生), Yan P(阎品), Abei D-L(阿拜都拉), Yang K-G(杨夸鼓), Ding X-Z(丁巷柱). The reasons and strategies of the sugar content decreased of sugarbeet in Tacheng Basin, Xinjiang. Sugar Crops China (中国糖料), 2002, (1): 42–44(in Chinese)

[2] Hadacek F, Kraus G F. Plant root carbohydrates affect growth behaviour of endophytic microfungi. FEMS Microbiol Ecol, 2002, 41: 161–170

[3] Vila-Aiub M M, Gundel P E, Ghersa C M. Fungal endophyte infection changes growth attributes in Lolium multiflorum Lam. Austral Ecol, 2005, 30: 49–57

[4] Ait Barka E, Gognies S. Inhibitory effect of endophyte bacteria on Botrytis cinerea and its influence to promote the grapevine growth. Biol Control, 2002, 24: 135–142

[5] Jacobs M J. Enumeration, location, characterization of endophytic bacteria within sugar beet roots. Can J Bot, 1985, 63: 1262–1265

[6] Larran S, Monaco C, Alippi H. Endophytic fungi in beet (Beta vulgaris var. esculenta L.) leaves. Adv Hort Sci, 2004, (4): 193–196

[7] Yan C P, Ma F M, Gao J G. Research on glutamate synthase activity in sugar beet (Beta vulgaris L.). J Northeast Agric Univ, 1998, 5(1): 5–11

[8] Zhao Y(赵越), Wei Z-M(魏自民), Li C(李成), Ma F-M(马凤鸣). The relationship between the key enzyme of nitrogen metabolism and sugarbeet. J Northeast Agric Univ (东北农业大学学报), 2003, 34(4): 368–371(in Chinese with English abstract)

[9] Li Cai-M(李彩凤), Ma F-M(马凤呜), Zhao Y(赵越), Li W-H(李文华). Effects of nitrogen forms on key enzyme activities and related products in sugar and nitrogen metabolism of sugar beet (Beta vulgaris L.). Acta Agron Sin (作物学报), 2003, 29(1): 128–132(in Chinese with English abstract)

[10] Yu H-B(于海彬), Cai B(蔡葆), Sun G-Q(孙国琴), Wang Q(王秋). The research of NR activity. China Sugar Beet (中国糖料), 1993, (3): 18–23 (in Chinese with English abstract)

[11] Miflin B J, Lea P J. Amino acid metabolism. Annu Rev Plant Physiol, 1977, 28: 299–329

[12] Liu Q(刘强), Peng J-W(彭建伟), Rong X-M(荣湘民), Xie G-X(谢桂先), Zhu H-M(朱红梅). Effect of carboxymethyl chitosan on nitrogen metabolism of rice. Plant Nutr Fert Sci (植物营养与肥料学报), 2007, 13(4): 597–601(in Chinese with English abstract)

[13] Zhao Y(赵越), Wei Z-M(魏自民), Ma F-M(马凤鸣). Influence of ammoniacal nitrogen on sucrose synthase and sucrose phosphate synthase in sugar beet. Sugar Crops China (中国糖料), 2003, (3): 1–5(in Chinese)

[14] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, 72: 248–254

[15] Zhang X Z(张宪政). Research Methods on Crop Physiology (作物生理学研究方法). Beijing: Agriculture Press, 1992. p 142(in Chinese)

[16] Shao J W, Cai B. Sugar Beet Physiology (甜菜生理学). Beijing: Agricultural Press, 1991. pp 181–196(in Chinese)

[17] Naik M S, Abrol Y P, Nair T V R. Nitrate assimilation its regulation and relationship to reduced nitrogen in higher plants. Phytochemistry, 1982, 21: 495–504

[18] Tang Y-W(汤玉玮), Lin Z-W(林振武), Chen J-X(陈敬祥). Study on the correlation between nitrate reductase activity and nitrogen respinsein crop plant and its application in biochemical plant breeding. Sci Agric Sin (中国农业科学), 1985, 18(6): 39–45 (in Chinese with English abstract)

[19] Zhao Y(赵越), Wei Z-M(魏自民), Li C(李成), Ma F-M(马凤鸣). The relationship between the key enzyme of nitrogen metabolism and sugar beet yield and quantity. J Northeast Agric Univ (东北农业大学学报), 2003, 34(4): 368–371(in Chinese with English abstract)

[20] Onkar S, Kanwar R S. Nitrate reductase activity—a growth factor of Sugar cane leaves. Indian Sugar, 1989, 39: 159–163

[21] Mack G. Glutamine synthetase isoenzymes, oligomers and subunits from hairy roots of Beta vulgaris L. var. lutea. Planta, 1998, 205: 113–120

[22] Pavlinova O A. Sucrose synthesizing enzymes of the sugar beet roots. Fiziol Rast, 1970, 17: 295–301

[23] Shen D-L(沈德龙), Feng Y-J(冯永君), Song W(宋未). Effect of Pantoea agglomerans on the distribution of photosynthate in flag leaf and spike of rice sugar beet. Prog Nat Sci (自然科学进展), 2002, 12(8): 863–865(in Chinese with English abstract)

[24] Holland M A. Occam’s razor applied to hormonology: are cytokinnins produced by plants. Plant Physiol, 1997, 115: 865–868

[25] Xu Q-M(徐亲民), Gao J(高杰), Jin Z-L(金智俐), Huang J-D(黄爱娣). The microorganisms produce sugar by sugar crops agent. Patent: CN 971261660, 1997-12-31 (in Chinese with English abstract)

Related Articles 15

[1]	YU Chao, LI Guo-Long, SUN Ya-Qing, LI Ning-Ning, ZHANG Shao-Ying. Characteristics of respiratory metabolism in growth and development of sugar beet taproot [J]. Acta Agronomica Sinica, 2023, 49(12): 3377-3386.
[2]	ZHAO Xiao-Xin, HUANG Shuo-Qi, TAN Wen-Bo, XING Wang, LIU Da-Li. Identification and relative expression profile of HIPPs gene family cadmium stress in sugar beet [J]. Acta Agronomica Sinica, 2023, 49(12): 3302-3314.
[3]	ZHANG Jia-Kang, LI Fei, SHI Shu-De, YANG Hai-Bo. Construction and application of the critical nitrogen concentration dilution model of sugar beet in Inner Mongolia, China [J]. Acta Agronomica Sinica, 2022, 48(2): 488-496.
[4]	Chun-Yan HUANG,Wen-Bin SU,Shao-Ying ZHANG,Fu-Yi FAN,Xiao-Xia GUO,Zhi LI,Cai-Yuan JIAN,Xiao-Yun REN,Qian-Heng GONG. Effects of Potassium Application on Photosynthetic Performance, Yield, and Quality of Sugar Beet with Mulching-drip Irrigation [J]. Acta Agronomica Sinica, 2018, 44(10): 1496-1505.
[5]	LI Zhi,LI Guo-Long,ZHANG Yong-Feng,YU Chao,SU Wen-Bin,FAN Fu-Yi,ZHANG Shao-Ying. Physiological Indices of High-Yielding Sugar Beet Under Drip Irrigation and Plastic Mulching [J]. Acta Agron Sin, 2017, 43(11): 1724-1730.
[6]	LI Yang-Yang,FEI Cong,CUI Jing,WANG Kai-Yong,MA Fu-Yu,FAN Hua. Compensation Response of Drip-Irrigated Sugar Beets (Beta vulgaris L.) to Different Water Deficits during Storage Root Development [J]. Acta Agron Sin, 2016, 42(11): 1727-1732.
[7]	LI Wei,SHEN Jia-Heng,GUO De-Dong. Ultrastructure of Central Cell before and after Fertilization in Sugar Beet (Beta vulgaris) [J]. Acta Agron Sin, 2014, 40(01): 166-173.
[8]	LI Wei,SHEN Jia-Heng,GUO De-Dong. Ultrastructure of Synergid in Its Degenerative Process in Sugar Beet (Beta vulgaris) [J]. Acta Agron Sin, 2013, 39(12): 2220-2227.
[9]	MA Lan,DU Hong-Yan,LI Rong-Tian. Seed-Setting Mode of Monosomic Addition Line M14 of Sugar Beet Beta corolliflora [J]. Acta Agron Sin, 2013, 39(03): 381-388.
[10]	WANG Mao-Qian, WU Ze-Dong, WANG Hua-Zhong. Analysis of Genetic Diversity of Major Sugar Beet Varieties from Three Regions of China with SRAP Markers [J]. Acta Agron Sin, 2011, 37(05): 811-819.
[11]	DING Chang-Hong,SHEN Jia-Heng,GUO De-Dong,SHANG Ya-Jia,LU Jun-Ping. Ultrastructure of Megasprogenesis in Faculative Apomictic Monosonic Addition Line M14 of Beta corolliflora of Sugar Beet [J]. Acta Agron Sin, 2009, 35(8): 1516-1524.
[12]	CHEN Sheng-Yong;HOU Jing;LI Cai-Feng;MA Feng-Ming;YIN Chun-Jie; HUANG Zhao-Feng. Influence of Inhibitors of Nucleic Acid Synthesis and Protein Synthesis on Glutamine Synthetase Gene Expression Induced by Nitrogen in Sugar Beet (Beta vulgaris L.) [J]. Acta Agron Sin, 2009, 35(3): 445-451.
[13]	WANG Hua-Zhong;WU Ze-Dong;WANG Xiao-Wu;FANG Zhi-Yuan. Analysis of the Genetic Diversity in Different Types of Sugar Beets by SRAP and SSR Markers [J]. Acta Agron Sin, 2008, 34(01): 37-46.
[14]	WANG Hua-Zhong;ZHANG Wen-Bin. Effect of Hypocotyl Color Selection of Sugar Beet on Generation and Crosses [J]. Acta Agron Sin, 2007, 33(06): 1029-1033.
[15]	SHEN Ye; SHEN Jia-Heng; GUO De-Dong; FANG Xiao-Hua and LIU Li-Ping. Dynamics of Callose Deposition in Cell Walls during Megasporogenesis in the Apomictic Monosomic Addition Line M14 of Beta corolliflora of Sugar Beet [J]. Acta Agron Sin, 2006, 32(06): 894-898.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Effects of Endophytic Fungus on Sugar Content and Key Enzymes Activity in Nitrogen and Sugar Metabolism of Sugar Beet(Beta vulgaris L.)

PDF

Cited