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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (05): 811-819.doi: 10.3724/SP.J.1006.2011.00811


Analysis of Genetic Diversity of Major Sugar Beet Varieties from Three Regions of China with SRAP Markers

WANG Mao-Qian1,2,3, WU Ze-Dong1,2,3, WANG Hua-Zhong1,2,3,*   

  1. 1 The Key Laboratory of Sugar Beet Genetic Breeding, Colleges of Heilongjiang Province / Heilongjiang University, Harbin 150080, Heilongjiang; 2 The Key Laboratory of North Sugar Crop Resource and Utilization, Chinese Academy of Agricultural Sciences, Harbin 150080, Heilongjiang; 3 Sugar Beet Research Institute of Chinese Academy of Agricultural Sciences / Crop Academy of Heilongjiang University, Harbin 150080, Heilongjiang
  • Received:2010-10-08 Revised:2011-01-06 Online:2011-05-12 Published:2011-03-24
  • Contact: 王华忠, E-mail: wwhhzz0451@sohu.com, Tel: 0451-86609494

Abstract: Shortage ofsugar beet germlasm resources results in the lag in researches of breeding and molecular biology. It is necessary to analyze the major varieties from three major production regions of China. Eighty-eight primer pairs were used to amplify the genomic DNA from leaves of four types of sugar beet varieties with different economic traits, which contain high yield type; high yield, low sugar and Rhizomania resistant type; standard type; medium yield, high sugar and anti-brown spot type, and 33 of which were obtained to be with availability. Two hundred and forty-one varieties from three major regions of China and nine varieties from abroad were detected with 33 primer pairs of SRAP markers. A total of 719 unambiguous bands were obtained, 459 of which were polymorphic. The average ratio of polymorphic bands was 63.8%. Compute over-all mean showed that genetic distance was 0.4165, genetic similarity among varieties was 0.6593, the genetic similarities were 0.7528 among foreign varieties, 0.6945 among monogerm varieties, 0.6816 among polygerm tetraploid varieties, and 0.6612 among polygerm diploid varieties. A total of 250 varieties were divided into four cluster groups based on cluster analysis by MEGA3.1 (at intercept of 0.2). Each genepool from three major region showed the highest. The varieties from China and abroad were classified into two different groups by POPGEN32. This indicated definite difference in the genetic background between foreign and native varieties.s of sugar beet production in China showed high level of genetic diversity, of which the Northeast genepool

Key words: Sugar beet, SRAP marker, Germplasm resource, Genetic diversity

[1]Lu Y-C(路运才), Wang H-Z(王华忠). Genetic analysis of sugarbeet polyploid varieties by RAPD markers. Sugar Crops China (中国糖料), 2006, (3): 5−8 (in Chinese with English abstract)
[2]Yu X(于歆), Xu D-C(徐德昌), Cui J(崔杰), Wu Y-Y(吴永英), Xia H-M(夏红梅). AFLP relationship and genealogy analysis of sugar beet sumfull series varieties (lines). Mol Plant Breed (分子植物育种), 2004, 2(2): 229−234 (in Chinese with English abstract)
[3]Tian Z-H(田自华), Zhang Z-Y(张子义), Zhang J-F(张剑峰), Shi S-D(史树德), Bai W(白薇), Shao J-W(邵金旺). RAPD analysis of mitochondrial DNA of cytoplasmic male sterile line and its maintainer line in sugarbeet. Mol Plant Breed (分子植物育种), 2004, 2(6): 817−828 (in Chinese with English abstract)
[4]Tian Z-H(田自华), Shi S-D(史树德), Zhang J-F(张剑峰), Zhang Z-Y(张子义), Shao J-W(邵金旺). RAPD analysis of chloroplast DNA of cytoplasmic male sterile line and its maintainer line in sugarbeet. Acta Agric Boreali-Sin (华北农学报), 2004, 19(1): 54−56 (in Chinese with English abstract)
[5]Wang H-Z(王华忠), Wu Z-D(吴则东), Wang X-W(王晓武), Fang Z-Y(方智远). Analysis of the genetic diversity in different types of sugar beets by SRAP and SSR Markers. Acta Agron Sin (作物学报), 2008, 34(1): 37−46 (in Chinese with English abstract)
[6]Wang H-Z(王华忠), Wu Z-D(吴则东), Han Y(韩英), Wang C-L(王丛玲). Optimization of sugarbeet SRAP-PCR reaction system. Sugar Crops China (中国糖料), 2007, (2): 1−4 (in Chinese with English abstract)
[7]Tautz D. Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucl Acids Res, 1989, 17: 6463−6471
[8] Liu L-W(柳李旺), Gong Y-Q(龚义勤), Huang H(黄浩), Zhu X-W(朱献文). A new molecular marker-SRAP and TRAP and its application. Hereditas (遗传), 2004, 26(5): 777−781 (in Chinese with English abstract)
[9]Budak H, Shearman R C, Parmaksiz I, Gaussoin R E, Riordan T P, Dweikat I. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers. Theor Appl Genet, 2004, 108: 328−334
[10]Lin Z-X(林忠旭), Zhang X-L(张献龙), Nie Y-C(聂以春), He D-H(贺道华), Wu M-Q(吴茂清). Construction of SRAP linkage map in cotton. Chin Sci Bull (科学通报), 2003, 48(15): 1676−1679 (in Chinese with English abstract)
[11]Lin Z-X(林忠旭), Zhang X-L(张献龙), Nie Y-C(聂以春). Evaluation of application of a new molecular marker SRAP on analysis of F2 segregation population and genetic diversity in cotton. Acta Genet Sin (遗传学报), 2004, 31(6): 622−626 (in Chinese with English abstract)
[12]Li Y(李严), Zhang C-Q(张春庆). Studies on genetic diversity with a molecular marker SRAP of watermelon hybrids. Acta Hort Sin (园艺学报), 2005, 32(4): 643−647 (in Chinese with English abstract)
[13]Wen Y-C(文雁成), Wang H-Z(王汉中), Shen J-X(沈金雄), Liu G-H(刘贵华), Zhang S-F(张书芬). Analysis of genetic diversity and genetic basis of Chinese rapeseed cultivars (Brassica napus L.) by sequence-related amplified polymorphism markers. Sci Agric Sin (中国农业科学), 2006, 39(2): 246−256 (in Chinese with English abstract)
[14]Liu Y-G(刘月光), Teng Y-Y(滕永勇), Pan C(潘辰), Han Y-C(韩延闯), Zhou M-Q(周明全), Hu Z-L(胡中立). Cluster analysis of Nelumbo based on SRAP markers. Amino Acids Biotic Res (氨基酸和生物资源), 2006, 28(1): 29−32 (in Chinese with English abstract)
[15]Wu J(吴洁), Tan W-F(谭文芳), He J-R(何俊蓉), Pu Z-G(蒲志刚), Wang D-Y(王大一), Zhang Z-S (张正圣), Zhan F-F(詹付凤), Yan W-Z(阎文昭). Construct on of SRAP linkage map and QTL mapping for starch content in sweet potato. Mol Plant Breed (分子植物育种), 2005, 3(6): 841−845 (in Chinese with English abstract)
[16]Budak H, Shearman R C, Parmaksiz I, Gaussoin R E, Riordan T P, Dweikat I. Molecular characterization of buffalograss germplasmusing sequence-related amplified polymorphism markers. Theor Appl Genet, 2004, 108: 328−334
[17]Chen K-S(陈昆松), Li F(李方), Xu C-J(徐昌杰), Zhang S-L(张上隆), Fu C-X(傅承新). An efficient macro- method of genomic DNA isolation from Actinidia chinensis leaves. Hereditas (遗传), 2004, 26(4): 529−531 (in Chinese with English abstract)
[18]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet, 2001, 103: 455−461
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