Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (05): 820-831.doi: 10.3724/SP.J.1006.2011.00820

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Protein Response of Rice Leaves to High Temperature Stress and Its Difference of Genotypes at Different Growth Stage

ZHOU Wei-Hui1,XUE Da-Wei2,ZHANG Guo-Ping1,*   

  1. 1 Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China; 2 College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China
  • Received:2010-10-22 Revised:2011-01-06 Online:2011-05-12 Published:2011-03-24
  • Contact: 张国平, E-mail: zhanggp@zju.edu.cn

PDF

1906

Cited

9

Abstract: High temperature has become a major disastrous factor affecting rice productivity, and the temperature stress becomes more severe due to the global warming effect. The present study was carried out to identify the rice genotypes with high heat tolerance and understand the tolerant mechanisms of both physiology and proteomics. The results showed that seed setting rate, SPAD value, plant height, root length and biomass were dramatically reduced under high temperature, while contents of malondialdehyde, hydrogen peroxide and superoxide anions, and activity of superoxide dismutase were greatly increased, irrespectively of growth stage. Moreover, Minghui 63 (a heat-sensitive genotype) was much more affected by heat stress than Milyang 46 (a heat-tolerant genotype). Proteomic analysis showed that high temperature resulted in down-regulation of the proteins related to photosynthesis, energy and metabolism, while resistance-related proteins were up-regulated. The results also confirmed the heat tolerance of Milyang 46 and the heat sensitivity of the rice plants at heading stage. The up-regulation of anti-stress protein, 2-cys peroxiredoxin BAS1, under heat stress was first reported in this study.

Key words: Rice, High temperature, Thermo-tolerance, Genotype, Proteomics

[1]Wilkins M R, Williams K L, Appel R D, Hochstrasser D F. Proteome Research, New Frontiers in Functional Genomics. Berlin: Springer, 1997. pp 304-331
[2]Süle A, Vanrobaeys F, Hajós G, Van Beeumen J, Devreese B. Proteomic analysis of small heat shock protein isoforms in barley shoots. Phytochemistry, 2004, 65: 1853-1863
[3]Xu C P, Huang B R. Root proteomic responses to heat stress in two Agrostis grass species contrasting in heat tolerance. J Exp Bot, 2008, 59: 4183-4194
[4]Yan S P, Zhang Q Y, Tang Z C, Su W A, Sun W N. Comparative proteomic analysis provides new insights into chilling stress responses in rice. Mol Cell Proteomics, 2006, 5: 484-496
[5]Pinheiro C, Kehr J, Ricardo C P. Effects of water stress on lupin stem protein analysed by two-dimensional gel electrophoresis. Planta, 2005, 221: 716-728
[6]Wang S B, Chen F, Sommerfeld M, Hu Q. Proteomic analysis of molecular response to oxidative stress by the green alga Haematococcus pluvialis (Chlorophyceae). Planta, 2004, 220: 17-29
[7]Yan S, Tang Z, Su W, Sun W. Proteomic analysis of salt stress-responsive proteins in rice root. Proteomics, 2005, 5: 235-244
[8]Labra M, Gianazza E, Waitt R, Eberini I, Sozzi A, Grassi F, Agradi E. Zea mays L. protein changes in response to potassium dichromate treatments. Chemosphere, 2006, 62: 1234-1244
[9]Salekdeh G H, Siopongco J, Wade L J, Ghareyazie B, Bennett J. Proteomics analysis of rice leaves during drought stress and recovery. Proteomics, 2002, 2: 1131-1145
[10]Imin N, Kerim T, Weinman J J, Rolfe B G. Characterisation of rice anther proteins expressed at the young microspore stage. Proteomics, 2001, 1: 1149-1161
[11]Salekdeh G H, Siopongco J, Wade L J, Ghareyazieb B, Bennett J. A proteomics approach to analyzing drought- and salt-responsiveness in rice. Field Crops Res, 2002, 76: 199-219
[12]Agrawal G K, Rakwal R, Yonekura M, Kubo A, Saji H. Proteome analysis of differentially displayed proteins as a tool for investigating ozone stress in rice (Oryza sativa L.) seedlings. Proteomics, 2002, 2: 947-959
[13]Shen S, Jing Y, Kuang T. Proteomics approach to identify wound-response related proteins from rice leaf sheath. Proteomics, 2003, 3: 527-535
[14]Peng S, Huang J, Sheehy J E, Laza R C, Visperas R M, Zhong X H, Centeno G S, Khush G S, Cassman K G. Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci USA, 2004, 101: 9971-9975
[15]Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C. Transgenic rice for tolerance against abiotic stresses. In: Datta S K ed. Rice Improvement in the Genomic Era. USA: Hawarth Press, 2009. pp 237-267
[16]Lee D G, Ahsan N, Lee S, Kang K Y, Bahk J D, Lee I, Lee B. A proteomic approach in analyzing heat-responsive proteins in rice leaves. Proteomics, 2007, 7: 3369-3383
[17]Han F, Chen H, Li X J, Yang M F, Liu G S, Shen S H. A comparative proteomic analysis of rice seedlings under various high-temperature stresses. Biochim Biophys Acta, 2009, 1794: 1625-1634
[18]Satake T, Yoshida S. High temperature induced sterility in indica rices at flowering. Jpn J Crop Sci, 1978, 47: 6-17
[19]Jagadish S V K, Muthurajan R, Oane R, Wheeler T R, Heuer S, Bennett J, Craufurd P Q. Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.). J Exp Bot, 2010, 61: 143-156
[20]Lin S K, Chang M C, Tsai Y C, Lur H S. Proteomic analysis of the expression of proteins related to rice quality during caryopsis development and the effect of high temperature on expression. Proteomics, 2005, 5: 2140-2156
[21]Yoshida S, Forna D A, Cock J H, Gomez K A. Laboratory Manual for Physiological Studies of Rice. Philippines: International Rice Research Institute, 1976. pp 62-63
[22]Xu C-C(许长成), Zhao S-J(赵世杰), Zou Q(邹琦). Interference factors of thiobarbituric acid determination in plant membrane lipid peroxidation. Plant Physiol Commun (植物生理学通讯), 1993, 29(5): 361-363 (in Chinese)
[23]Ruan H-H(阮海华), Shen W-B(沈文飚), Ye M-B(叶茂炳), Xu L-L(徐朗莱). Effects of nitric oxide on oxidative injury in wheat leaves under salt stress. Chin Sci Bull(科学通报), 2001, 46(23): 1993-1997 (in Chinese)
[24]Lin C C, Kao C H. Abscisic acid induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. Plant Sci, 2001, 160: 323-329
[25]Li J Y, Jiang A L, Zhang W. Salt stress-induced programmed cell death in rice root tip cells. J Integr Plant Biol, 2007, 49: 481-486
[26]Wang J-Y(王经源), Chen S-Y(陈舒奕), Liang Y-Y(梁义元), Lin W-X(林文雄). Improvement of ISO-DALT electrophoresis system. J Fujian Agric & For Univ (福建农林大学学报), 2006, 35(2): 187-190 (in Chinese with English abstract)
[27]Yu C-L(余初浪), Yan S-P(严顺平), Sun W-N(孙卫宁), Yang L(杨玲). High-resolution two-dimensional electrophoresis for total proteins in rice roots, leaves and suspension cells. Chin J Rice Sci (中国水稻科学), 2006, 20(5):549-552 (in Chinese with English abstract)
[28]Yan J X, Wait R, Berkelman T, Harry R A, Westbrook J A, Wheeler C H, Dunn M J. A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass pectrometry. Electrophoresis, 2000, 21: 3666-3672
[29]Neuhoff V，Arnold N，Taube D，Ehrhardt W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis, 1988, 9: 255-262
[30]Makino A, Mae T, Ohira K. Photosynthesis and ribulose-1,5-bisphosphate carboxylase/oxygenase in rice leaves from emergence through senescence. Planta, 1985, 166: 414-420
[31]Bose A, Ghosh B. Effect of heat stress on ribulose 1,5-bisphosphate carboxylase in rice. Phytochemistry, 1995, 38: 1115-1118
[32]Hankamer B，Barber J，Boekema F J．Structure and membrane organization of photosystem II in green plants. Annu Rev Plant Physiol Plant Mol Biol, 1997, 48: 64l-671
[33]James B. Crystal structure of the oxygen-evolving complex of photosystem II. Inorg Chem, 2008, 47: 1700-1710
[34]Ahsan N, Lee D G, Lee K W, Alam I, Lee S H, Bahk J D, Lee B H. Glyphosate-induced oxidative stress in rice leaves revealed by proteomic approach. Plant Physiol Biochem, 2008, 46: 1062-1070
[35]Ahsan N, Lee D G, Lee S H, Kang K Y, Bahk J D, Choi M S, Lee I J, Renaut J, Lee B H. A comparative proteomic analysis of tomato leaves in response to waterlogging stress. Physiol Plant, 2007, 131: 555-570
[36]Wang Z Y, Freire E, McCarty R E. Influence of nucleotide binding site occupancy on the thermal stability of the F1 portion of the chloroplast ATP synthase. J Biol Chem, 1993, 268: 20785-20790
[37]Ahsan N, Donnart T, Nouri M Z, Komatsu S. Tissue-speci?c defense and thermo-adaptive mechanisms of soybean seedlings under heat stress revealed by proteomic approach. J Proteome Res, 2010, 9: 4189-4204
[38]Girardini J E, Dissous C, Serra E. Schistosoma mansoni ferredoxin NADP(H) oxidoreductase and its role in detoxi?cation. Mol Biochem Parasitol, 2002, 124: 37-45
[39]Wang C, Wesener S R, Zhang H L, Cheng Y Q. An FAD-dependent pyridine nucleotide-disul?de oxidoreductase is involved in disul?de bond formation in FK228 snticancer depsipeptide. Chem Biol, 2009. 16: 585-593
[40]Majoul T, Bancel E, Triboï E, Hamidal J B, Branlard G. Proteomic analysis of the effect of heat stress on hexaploid wheat grain: Characterization of heat-responsive proteins from total endosperm. Proteomics, 2003, 3: 175-183
[41]Rainwater D T, Gossetp D R, Millhollon E P, Hanna H Y, Banks S W, Lucas M C. The relationship between yield and the antioxidant defense system in tomatoes grown under heat stress. Free Radical Res, 1996, 25: 421-435
[42]Ai Q(艾青), Mou T-M(牟同敏). Progresses in rice heat tolerance. Hubei Agric Sci (湖北农业科学), 2008, 47(1): 107-111 (in Chinese with English abstract)
[43]Farrell T C, Fox K M, Williams R L, Fukai S. Genotypic variation for cold tolerance during reproductive development in rice: screening with cold air and cold water. Field Crops Res, 2006, 98: 178-194
[44]Dietz K J, Horling F, Konig J, Baier M. The function of the chloroplast 2-cysteine peroxiredoxin in peroxide detoxification and its regulation. J Exp Bot, 2002, 53: 1321-1329
[45]Baier M, Dietz K J. Alkyl hydroperoxide reductases: The way out of the oxidative breakdown of lipids in chloroplasts. Trends Plant Sci, 1999, 4: 166-168
[46]Baier M. Dietz K J. Protective function of chloroplast 2-cysteine peroxiredoxin in photosynthesis: Evidence from transgenic Arabidopsis. Plant Physiol, 1999, 119: 1407-1414
[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[3] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[4] ZHENG Xiao-Long, ZHOU Jing-Qing, BAI Yang, SHAO Ya-Fang, ZHANG Lin-Ping, HU Pei-Song, WEI Xiang-Jin. Difference and molecular mechanism of soluble sugar metabolism and quality of different rice panicle in japonica rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1425-1436.
[5] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[6] YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[7] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[8] YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[9] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[10] WANG Xiao-Lei, LI Wei-Xing, OU-YANG Lin-Juan, XU Jie, CHEN Xiao-Rong, BIAN Jian-Min, HU Li-Fang, PENG Xiao-Song, HE Xiao-Peng, FU Jun-Ru, ZHOU Da-Hu, HE Hao-Hua, SUN Xiao-Tang, ZHU Chang-Lan. QTL mapping for plant architecture in rice based on chromosome segment substitution lines [J]. Acta Agronomica Sinica, 2022, 48(5): 1141-1151.
[11] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[12] KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[13] CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.
[14] WANG Lyu, CUI Yue-Zhen, WU Yu-Hong, HAO Xing-Shun, ZHANG Chun-Hui, WANG Jun-Yi, LIU Yi-Xin, LI Xiao-Gang, QIN Yu-Hang. Effects of rice stalks mulching combined with green manure (Astragalus smicus L.) incorporated into soil and reducing nitrogen fertilizer rate on rice yield and soil fertility [J]. Acta Agronomica Sinica, 2022, 48(4): 952-961.
[15] QIN Qin, TAO You-Feng, HUANG Bang-Chao, LI Hui, GAO Yun-Tian, ZHONG Xiao-Yuan, ZHOU Zhong-Lin, ZHU Li, LEI Xiao-Long, FENG Sheng-Qiang, WANG Xu, REN Wan-Jun. Characteristics of panicle stem growth and flowering period of the parents of hybrid rice in machine-transplanted seed production [J]. Acta Agronomica Sinica, 2022, 48(4): 988-1004.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd