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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (05): 820-831.doi: 10.3724/SP.J.1006.2011.00820

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Protein Response of Rice Leaves to High Temperature Stress and Its Difference of Genotypes at Different Growth Stage

ZHOU Wei-Hui1,XUE Da-Wei2,ZHANG Guo-Ping1,*   

  1. 1 Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China; 2 College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China
  • Received:2010-10-22 Revised:2011-01-06 Online:2011-05-12 Published:2011-03-24
  • Contact: 张国平, E-mail: zhanggp@zju.edu.cn

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Abstract: High temperature has become a major disastrous factor affecting rice productivity, and the temperature stress becomes more severe due to the global warming effect. The present study was carried out to identify the rice genotypes with high heat tolerance and understand the tolerant mechanisms of both physiology and proteomics. The results showed that seed setting rate, SPAD value, plant height, root length and biomass were dramatically reduced under high temperature, while contents of malondialdehyde, hydrogen peroxide and superoxide anions, and activity of superoxide dismutase were greatly increased, irrespectively of growth stage. Moreover, Minghui 63 (a heat-sensitive genotype) was much more affected by heat stress than Milyang 46 (a heat-tolerant genotype). Proteomic analysis showed that high temperature resulted in down-regulation of the proteins related to photosynthesis, energy and metabolism, while resistance-related proteins were up-regulated. The results also confirmed the heat tolerance of Milyang 46 and the heat sensitivity of the rice plants at heading stage. The up-regulation of anti-stress protein, 2-cys peroxiredoxin BAS1, under heat stress was first reported in this study.

Key words: Rice, High temperature, Thermo-tolerance, Genotype, Proteomics

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