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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (03): 381-388.doi: 10.3724/SP.J.1006.2013.00381


Seed-Setting Mode of Monosomic Addition Line M14 of Sugar Beet Beta corolliflora

MA Lan1,2,**,DU Hong-Yan1,**,LI Rong-Tian1,*   

  1. 1 Key Laboratory of Molecular Biology, Heilongjiang University, Harbin150080, China; 2 Daqing Branch of Heilongjiang Academy of Agricultural Sciences, Daqing 163000, China
  • Received:2012-06-25 Revised:2012-11-16 Online:2013-03-12 Published:2013-01-04
  • Contact: 李荣田, E-mail: lirongtian07@yahoo.com.cn, Tel: 0451-86608243 E-mail:malan042999@163.com


A cross combination of M14 with green petiole ×B. vulgaris with red petiole was prepared to observe the dominant trait marker of hybridplantsand thegenetic correlation between the maternal plant and F1 generation of M14 based on the SSR analysis. The result showed that M14 had higher transmission rate of the alien chromosome. Multiplied seeds came from the union of male and female gametes under artificial pollination, and the petiole color of all the hybrid plants was red. There was no the exactly identical genotype in the progeny compared with the maternal plant. It indicated that the seed-setting mode of M14 is not apomixis, but sexual reproduction. Southern blot showed that the chromosomes of B. vulgaris reversed from M14 sharing the DNA sequence ofB. corolliflora,it is implying that there was popular meiosis and chromosome aberration during the female gamete formation of sugar-beet M14. The study came to the conclusion that higher transmission frequency of the alien chromosome of sugar-beet M14 is probably induced by the chromosome 9 of Beta corolliflora with gametocidal function.

Key words: Monosomic addition line M14 in sugar beet, Apomixis, Sexual reproduction, Hybridization, Genetic correlation, Southern blot

[1]Bicknell R A, Koltunow A M. Understanding apomixis: recent advances and remaining conundrums. Plant Cell, 2004, 16: 228–245

[2]Vielle-Calzada J P, Charles F G, David M S. Apomixis: the asexual revolution. Science, 1996, 274: 1322–1323

[3]Fang X H, Gu S H, Xu Z Y, Chen F, Guo D D. Construction of a binary BAC library for an apomictic monosomic addition line of Beta corolliflora in sugar beet and identification of the clones derived from the alien chromosome. Theor Appl Gent, 2004, 108: 1420–1425

[4]Yu B(于冰), Li H-Y(李海英), Ma C-Q(马春泉), Zhang S-J(张绍军), Li R-T(李荣田), Guo D-D(郭德栋). Construction of the subtractive cDNA library of buds of the apomictic monosomic addition line in sugar beet. High Technol Lett (高技术通讯), 2006, 16(9): 954–958 (in Chinese with English abstract)

[5]Ma C-Q(马春泉), Zhang Y(张莹), Cui Y(崔颖), Li H-Y(李海英). Construction of cDNA library in florescence and screening specifically expressed genes in sugar beet M14. Bull Bot Res (植物研究), 2008, 28(4): 408–411(in Chinese with English abstract)

[6]Ge Y, He G C, Guo D D, Li R T. GISH and BAC-FISH study of apomictic Beta M14. Sci in China: Series C Life Sci, 2007, 50: 242–250

[7]Ma C, Wang Y, Wang L, Chen S, Li H. Identification of a sugar beet BvM14-MADS box gene through differential gene expression analysis of monosomic addition line M14. J Plant Physiol, 2011, 168: 1980–1986

[8]Li H Y, Cao Y G, Wang Y G, Ma C Q, Chen S X. Proteomic analysis of sugar beet apomictic monosomic addition line M14. J Proteomics, 2009, 73: 297–308

[9]Zhu H, Bi Y D, Yu L J, Guo D D, Wang B C. Comparative proteomic analysis of apomictic monosomic addition line of Beta corolliflora and Beta vulgaris L. in sugar beet. Mol Biol Rep, 2009, 36: 2093–2098

[10]Guo D-D(郭德栋), Li S-X(李盛贤), Jia S-B(贾树彪), Kang C-H(康传红), Li Y(李勇). Studies on the interspecific hybridization in genus Beta. Sugar Crops China (中国糖料), 1999, (2): 1–8 (in Chinese with English abstract)

[11]Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍), Kang C-H(康传红), Li Y(李勇), Wang G-Z(王桂芝). Acquisition and identification of monosomic additional lines of apomictic beet. J Yunnan Univ (云南大学学报), 2000, 34(5): 1–3 (in Chinese with English abstract)

[12]Guo D-D(郭德栋), Liu L-P(刘丽萍), Kang C-H(康传红). The transmission and reproductive characters of apomictic monosomic addition lines of Beta vulgaris L. J Nat Sci Heilongjiang Univ (黑龙江大学自然科学学报), 2001, 18(3): 104–107 (in Chinese with English abstract)

[13]Gao D-J(高东杰), Kang C-H(康传红), Liu L-P(刘丽萍), Guo D-D(郭德栋), Fang X-H(方晓华). A full set of monosomic addition lines in Beta vulgaris from B. corolliflora. Sugar Crops China (中国糖料), 2001, (4): 5–9 (in Chinese with English abstract)

[14]Jassem B. Apomixis in the Genus Beta. Apomixis Newslett, 1990, (2): 7–23

[15]Kang C-H(康传红), Wang G-Z(王桂芝), Jia S-B(贾树彪), Guo D-D(郭德栋), Liu L-P(刘丽萍). Observation on apomixes of progenies of interspecific hybridization between Beta vulgaris L. and Beta corollifloro. China Sugarbeet (中国甜菜), 1995, (1): 3–8 (in Chinese with English abstract)

[16]Li Y(李勇), Liu W(刘威), Kang C-H(康传红), Jia S-B(贾树彪), Guo D-D(郭德栋). Morphological and isozyme variations of apomictic lines from hybrid progenies between B. vulgaris L. and B. corollifloro. Sugar Crops China (中国糖料), 1997, (2): 6–9 (in Chinese with English abstract)

[17]Guo D-D(郭德栋), Kang C-H(康传红), Liu L-P(刘丽萍), Li Y(李勇), Fang X-H(方晓华). Study of apomixis in the allotriploid beet (VVC). Sci Agric Sin (中国农业科学), 1999, 32(4): 1–5 (in Chinese with English abstract)

[18]Kang C-H(康传红), Liu L-P(刘丽萍), Shen Y(申业), Guo D-D(郭德栋), Fang X-H(方晓华). Studies on apomict transmission rate in allotriploid beet (VVC). Sugar Crops China (中国糖料), 1999, (3): 1–5 (in Chinese with English abstract)

[19]Guo D-D(郭德栋), Li S-X(李盛贤), Jia S-B(贾树彪), Kang C-H(康传红), Li Y(李勇). Studies on the Interspecific Hybridization in Genus Beta. Sugar Crops China (中国糖料), 1999, (2): 1–6 (in Chinese with English abstract)

[20]Shen Y(申业), Shen J-H(申家恒), Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍). Dynamics of callose deposition in cell wall during megasporogenesis in the apomictic monosomic addition line M14 of Beta corolliflora of sugar beet. Acta Agron Sin (作物学报), 2006, 32(6): 894–898 (in Chinese with English abstract)

[21]Shen Y(申业), Shen J-H(申家恒), Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍). Cyto-embryological study of the apomictic monosomic addition line M14 in sugar beet. Acta Bot Boreali-Occident Sin (西北植物学报), 2006, 26(5): 957–963 (in Chinese with English abstract)

[22]Shen Y(申业), Shen J-H(申家恒), Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍). Megasporogenesis and development of female gametophyte in the apomictic monosomic addition line M14 of Beta corolliflora in sugar beet. J Wuhan Bot Res (武汉植物学研究), 2006, 24(2): 106–113 (in Chinese with English abstract)

[23]Ding C-H(丁常宏), Shen J-H(申家恒), Guo D-D(郭德栋), Shang Y-J(尚娅佳). Ultrastructure of megasporogenesis in facultative apomictic monosonic addition line M14 of Beta corolliflora of sugar beet. Acta Agron Sin (作物学报), 2009, 35(8): 1516−1524 (in Chinese with English abstract)

[24]Shang Y-J(尚娅佳), Shen J-H(申家恒), Guo D-D(郭德栋), Ding C-H(丁常宏). Ultrastructural characterization of the mature embryo sac in the chromosome-9 monosomic addition line of Beta corolliflora in sugar beet. Chin Bull Bot (植物学报), 2009, 44(6): 682–693 (in Chinese with English abstract)

[25]Shang Y-J(尚娅佳), Shen J-H(申家恒), Guo D-D(郭德栋), Ding C-H(丁常宏). The ultrastructural changes of female gametophyte during Its development in the apomictic monosomic addition line M14 of Beta corolliflora in sugar beet. Sci Agric Sin (中国农业科学), 2010, 43(1): 29–38 (in Chinese with English abstract)

[26]Gao D J, Schmidt T, Jung C. Molecular characterization and chromosomal distribution of species-specific repetitive DNA sequences from Beta corolliflora, a wild relative of sugar beet. Genome, 2000, 43: 1073–1080

[27]Li R T, Zhang Z M, Zhang Q F. Transformation of japonica rice with RHL gene and salt tolerance of the transgenic rice plant. Chin Sci Bull (科学通报), 2002, 47(8): 613–617

[28]Maguire M P. High transmission frequency of a tripsacum chromosome in corn. Genetics, 1963, 48: 1185–1194

[29]Nogler G A. Genetics of apomictic in Ranuncuclu auricomus: VI. Epilogue. Bot Helvet, 1995, 105, 111–115

[30]Bracaccia G, Mazzucato A, Belardinelli A. Inheritance of parental genomes in progenies of Poa pratensis L. from sexual and apomictic genotypes as assessed by RAPD markers and flow cytometry. Theor Appl Genet, 1997, 95: 516–524

[31]Kang C-H(康传红), Han X-Y(韩晓云), Wang Z-W(王志伟), Guo D-D(郭德栋), Wang G-Z(王桂芝). RAPD analysis to confirm the identification of three progenies of M14 beta vulgaris additional lines. Biotechnology (生物技术), 2002, (4): 9–11 (in Chinese with English abstract)

[32]Endo T R. Gametocidal chromosomes and their induction of chromosome mutations in wheat. Jpn J Genet, 1990, 65: 135–152

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