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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (03): 381-388.doi: 10.3724/SP.J.1006.2013.00381

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Seed-Setting Mode of Monosomic Addition Line M14 of Sugar Beet Beta corolliflora

MA Lan1,2,**,DU Hong-Yan1,**,LI Rong-Tian1,*   

  1. 1 Key Laboratory of Molecular Biology, Heilongjiang University, Harbin150080, China; 2 Daqing Branch of Heilongjiang Academy of Agricultural Sciences, Daqing 163000, China
  • Received:2012-06-25 Revised:2012-11-16 Online:2013-03-12 Published:2013-01-04
  • Contact: 李荣田, E-mail: lirongtian07@yahoo.com.cn, Tel: 0451-86608243 E-mail:malan042999@163.com

Abstract:

A cross combination of M14 with green petiole ×B. vulgaris with red petiole was prepared to observe the dominant trait marker of hybridplants,and thegenetic correlation between the maternal plant and F1 generation of M14 based on the SSR analysis. The result showed that M14 had higher transmission rate of the alien chromosome. Multiplied seeds came from the union of male and female gametes under artificial pollination, and the petiole color of all the hybrid plants was red. There was no the exactly identical genotype in the progeny compared with the maternal plant. It indicated that the seed-setting mode of M14 is not apomixis, but sexual reproduction. Southern blot showed that the chromosomes of B. vulgaris reversed from M14 sharing the DNA sequence ofB. corolliflora,it is implying that there was popular meiosis and chromosome aberration during the female gamete formation of sugar-beet M14. The study came to the conclusion that higher transmission frequency of the alien chromosome of sugar-beet M14 is probably induced by the chromosome 9 of Beta corolliflora with gametocidal function.

Key words: Monosomic addition line M14 in sugar beet, Apomixis, Sexual reproduction, Hybridization, Genetic correlation, Southern blot

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