Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1280-1285.doi: 10.3724/SP.J.1006.2010.01280

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Resynthesis of Brassica napus with Brassica oleracea or Brassica rapa Cytoplasm

LI Jun,LUO Li-Xia,WANG Zhuan,LI Jun,CHEN Kun-Rong,REN Li,FANG Xiao-Ping*   

  1. Oil CropsResearch Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Wuhan 430062, China
  • Received:2010-02-03 Revised:2010-04-20 Online:2010-08-12 Published:2010-05-20
  • Contact: FANG Xiao-Ping,E-mail:xpfang2008@163.com;xpfang@public.wh.hb.cn

Abstract: Nuclear genome is the primary genetic material, which plays a key role in the individual development and the system evolution of plant. Although relatively independent genetic system in cytoplasm is existed, the characteristics of plant is more often determined by the nuclear genes and sometimes affected by plasmagenes. To compare genetic effect of different plasmagenes of B. napus and study interactions between nuclear gene and cytogene, we artificially synthesized B. napus with B. oleracea or B. rapa cytoplasm by means of hybrid embryo and ovary culture of reciprocal crosses between 12 cultivated varieties of B. rapa and one cultivated variety of B. oleracea var. alboglabra.The average frequencies of hybrid plantlets obtained were 2.32% from ovary culture of B. rapa × B. oleracea var. alboglabra and 1.16% from embryo culture of B. oleracea var. alboglabra × B. rapa, respectively. The induction rate of hybrid plantlets differed greatly between different crosses, but the trends of induction easily or hardly were the same between reciprocal crosses with the same parents. The haploidic hybrid seedlings were pre-cultured in MS medium with 0.01% colchicine for 10 days which was the best way for chromosome doubling. The percentage of hybrid plantlets with doubled chromosomes was 59.32%. Agronomic traits of synthesized B. napus were similar to those of cultivated B. napus and mediated between parents in morphology, but more similar to maternal plants. The average pollen fertility for the most synthesized lines ranged from 40.53% to 88.95%.

Key words: Resynthesis of Brassica napus, Interspecific hybridization, Embryo cultur, Ovary culture

   [1]    Liu H-L(刘后利). Genetics and Breeding of Rapeseed (油菜的遗传和育种). Shanghai: Shanghai Scientific Technology Press, 1985 (in Chinese)   
[2]    Niu Y-Z(牛应泽), Wang L-Z(汪良中), Niu Y-Z(刘玉贞), Guo S-X(郭世星). Development of new germplasm in rapeseed through resynthesis of new Brassica napus L. Chin J Oil Crop Sci (中国油料作物学报), 2003, 25(2): 11–15 (in Chinese with English abstract)   
[3]    Liu P-W(刘平武), Yang G-S(杨光圣). Analyses of the genetic diversity of resynthesized Brassica napus by RAPD and SSR molecular markers. Acta Agron Sin (作物学报–1273 (in Chinese with English abstract)), 2004, 30(12): 1266   
[4]    Heath D W, Earle E D. Resynthesis of rapeseed (Brassica napus L.): a comparison of sexual versus somatic hybridization. Plant Breed, 1996, 115: 395–401   
[5]    Zhou W-J(周伟军), Tang G-X(唐桂香), Zhang G-Q(张国庆), Hagberg P. Studies on efficient production of doubled haploid plants by colchincine treatment in microspore culture of Brassica napus. Sci Agric Sin (中国农业科学), 2002, 35(4): 410–414 (in Chinese with English abstract)   
[6]    Li Z, Liu H L, Luo P. Production and cytogeneric hybrids between Brassica napus and Orychophragmus violaceus. Theor Appl Genet–136, 1995, 91: 131   
[7]    Zhang X-W(张晓伟), Gao M-Q(高睦枪), Yuan Y-X(原玉香), Geng J-F(耿建峰), Wen Y-C(文雁成), Zhang S-F(张书芬), Li G-Y(栗根义). Studies河南农业科学), 2001, (2): 7–10 (in Chinese with English abstract) on artificially synthesized B. napus L. J Henan Agri Sci (   
[8]    Lu C M, Zhang B, Kakihara F, Kato M. Introgression of genes into cultivated Brassica napus through resynthesis of B. napus via ovule culture and the accompanying change in fatty acid composition. Plant Breed, 2001, 120: 405–410   
[9]    Dale P J. Spread of engineered genes to wild relatives. Plant Physiol, 1992, 100: 13–15
[10]    Zhang G Q, Tang G X, Song W J, Zhou W J. Resynthesizing Brassica napus from interspecific hybridization between Brassica rapa and B. oleracea through ovary culture. Euphytica, 2004, 140: 181–187
[11]    Goy P A, Duesing J H. Assessing the environmental impact of gene transfer to wild relatives. Biotechnology, 1996, 14: 39–40
[12]    Hossain M M, Inden H, Asahira T. Intergeneric and interspecific hybrids through in vitro ovule culture in the Creuciferae. Plant Sci, 1988, 58: 121–128
[13]    Zhou Q-Y(周清元), Li J-N(李加纳), Yin J-M(殷家明), Tang Z-L(唐章林), Chen L(谌利), Tao L(陶澜). Preliminary studies on interspecific hybridization between Brassica campestris and B. oleracea var. aceaphala: I. The effect of the time of overay taking on seed forming in in-vitro culture. J Southwest Agric Univ (Nat Sci Edn)(西南农业大学学报·自然科学版), 2003, 25(6): 495–497 (in Chinese with English abstract)
[14]    Wen Y-C(文雁成), Zhang S-F(张书芬), Wang J-P(王建平), Li G-Y(栗根义), Zhang X-W(张晓伟). Studies on synthetic Brassica napus by interspecific hybridization between B. oleracea and B. campestris. Chin J Oil Crop Sci (中国油料作物学报), 1999, 21(4): 8–11 (in Chinese with English abstract)
[15]    Dai L-J(戴林建), Li X(李栒), Zhang S-W(张四伟). Crossabilities between Brassica spp. and Eruca sativa. Crop Res (作物研究), 2002, 3: 123–125 (in Chinese)
[16]    Scheffler J A, Dale P J. Opportunities for gene transfer from transgenic oilseed rape (Brassica napus) to related species. Transgenic Res, 1994, 3: 263–278
[17]    Wang D(王丹), Wang W(王文), Shi Y-Y(史滟滪). Embryologic research into reciprocal crosses between Brassica juncea and Bpekinesi. Chin Bull Bot (植物学通报), 2006, 23(2): 158–163 (in Chinese with English abstract)
[18]    Li J(李俊), Zhang C-L(张春雷), Li G-M(李光明). Research progress in gene flow from transgenic rapeseed (Brassica napus) to its relatives. Acta Agric Jiangxi (江西农业学报), 2009, 21(3): 32–37 (in Chinese with English abstract) Li M-T(栗茂腾), Liu J-M(刘建民), Wang Y-T(王艳婷), Yu L-J(余龙江), Meng J-L(孟金陵). Production of partial new-typed Brassica napus by introgression of genomic components from B. rapa and B. carinata. Acta Gen Sin (遗传学报), 2007, 34(5): 460–480 (in Chinese with English abstract)
[1] WANG Ai-Yun;LI Xun;HU Da-You. Production and Characterization of Intergeneric Hybrids between Bras-sica Species (B. juncea, B. nigra) and Orychophragmus violaceus by Ovary Culture [J]. Acta Agron Sin, 2008, 34(09): 1557-1562.
[2] PANG Chao-You ;DU Xiong-Ming;MA Zhi-Ying. Cluster Analysis of the Introgressed Lines from Interspecific Hybridization in Cotton based on SSR Markers and Phenotype Traits [J]. Acta Agron Sin, 2006, 32(09): 1371-1378.
[3] PAN Guang-Tang;ZHANG Zhi-Ming;WEI Xin;SONG Yun;ZHAO Mao-Jun;XIA Yan-Li;RONG Ting-Zhao. QTL Analysis of Maize (Zea mays L.) Embryo Culturing Capacity [J]. Acta Agron Sin, 2006, 32(01): 7-13.
[4] Luo Xin-tan;Guo fu-xing;Zhou Jun-yan;Ma Hui;Wu Qun-ming;Zhu Guang-qin;Ma Yun-tong. Immature Embryo Culture of a Cross between Setaria italica(Ch4n) and S.faberii and Studies on the Morphological and Cytological Characteristics of the F1 Plant [J]. Acta Agron Sin, 1993, 19(04): 352-358.
[5] Yuan Miaobao;Zhang Yufang; Zhu Muyuan; Xu Abing; Pei Hongping. Cytogenetic Studies on Barley Plants Regenerated from Immature Embryo Culture and Their Progenies [J]. Acta Agron Sin, 1991, 17(03): 171-177.
[6] Liang Zhuqing; Gao Mingwei; Cheng Xiongying. STUDY ON IN-VITRO TECHNIQUE FOR IMMATURE EMBRYO CULTURE OF WHEAT BREEDING [J]. Acta Agron Sin, 1988, 14(02): 137-142.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!