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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1280-1285.doi: 10.3724/SP.J.1006.2010.01280


Resynthesis of Brassica napus with Brassica oleracea or Brassica rapa Cytoplasm

LI Jun,LUO Li-Xia,WANG Zhuan,LI Jun,CHEN Kun-Rong,REN Li,FANG Xiao-Ping*   

  1. Oil CropsResearch Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Wuhan 430062, China
  • Received:2010-02-03 Revised:2010-04-20 Online:2010-08-12 Published:2010-05-20
  • Contact: FANG Xiao-Ping,E-mail:xpfang2008@163.com;xpfang@public.wh.hb.cn

Abstract: Nuclear genome is the primary genetic material, which plays a key role in the individual development and the system evolution of plant. Although relatively independent genetic system in cytoplasm is existed, the characteristics of plant is more often determined by the nuclear genes and sometimes affected by plasmagenes. To compare genetic effect of different plasmagenes of B. napus and study interactions between nuclear gene and cytogene, we artificially synthesized B. napus with B. oleracea or B. rapa cytoplasm by means of hybrid embryo and ovary culture of reciprocal crosses between 12 cultivated varieties of B. rapa and one cultivated variety of B. oleracea var. alboglabra.The average frequencies of hybrid plantlets obtained were 2.32% from ovary culture of B. rapa × B. oleracea var. alboglabra and 1.16% from embryo culture of B. oleracea var. alboglabra × B. rapa, respectively. The induction rate of hybrid plantlets differed greatly between different crosses, but the trends of induction easily or hardly were the same between reciprocal crosses with the same parents. The haploidic hybrid seedlings were pre-cultured in MS medium with 0.01% colchicine for 10 days which was the best way for chromosome doubling. The percentage of hybrid plantlets with doubled chromosomes was 59.32%. Agronomic traits of synthesized B. napus were similar to those of cultivated B. napus and mediated between parents in morphology, but more similar to maternal plants. The average pollen fertility for the most synthesized lines ranged from 40.53% to 88.95%.

Key words: Resynthesis of Brassica napus, Interspecific hybridization, Embryo cultur, Ovary culture

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