Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1270-1279.doi: 10.3724/SP.J.1006.2010.01270

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Screening Marker Genotypes with Elite Combining Ability for Yield Traits in Parents of Hybrid Japonica Rice (Oryza sativa L.)

LIANG Kui1, HUANG Dian-Cheng1,2,**, ZHAO Kai-Ming1, NGUYEN Phuong-Tung1,3,XIE Hui1,4,MA Wen-Xia,HONG De-Lin1,*   

  1. 1State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China;2Cotton Research Institute,chinese Academy of Agricultural Sciences,Anyang 455000,China;3Agricultural Science Institute of Northem Central Vietnam,Nghe An-Vinh,Vietnam;4Tianjin Subcentre of china National Hybrid Rice research and development Center,Tianjin 300457,China
  • Received:2010-02-01 Revised:2010-04-19 Online:2010-08-12 Published:2010-05-20
  • Contact: HONG De-LIn, E-mail: delinhong@njau.edu.cn, Tel: 025-84396626

Abstract: The area of japonica rice planted annually is 8 280 000 ha and accounts for 27% of total rice area in China. The area planting japonica hybrid rice is only 3% of the total area of japonica rice. The major reason for this situation is that competitive heterosis of hybrid cultivar is not conspicuous in yield, compared with conventional cultivar in japonica rice. The key factor of enhancing competitive heterosis of hybrid cultivar in japonica rice is to improve combining ability of yield related traits of restorer lines. In order to improve combining ability of restorer lines more efficiently, SSR marker genotypes with elite combining ability for five traits were screened in this study, by analyzing the data of combining ability and SSR markers in six CMS lines and twelve restorer lines that were genotyped using 115 pairs of SSR primers. Combining ability of the 18 parental lines was analyzed for daily yield per plant (DYP), panicles per plant (PP), total spikelets per panicle (TSP), filled spikelets per panicle (FSP) and 1000-grain weight (1000-GW), using the data of 72 F1s made with NC II genetic design. Results showed that twenty SSR marker genotypes were significantly associated with combining ability of the five traits. Among them, eight for only one trait, six for two traits, four for three traits and two for four traits. Marker genotypes associated with combining ability of multiple traits increased or decreased trait value of F1. RM152-165/170 was a marker genotype of elite combining ability for PP and DYP with the maximum increasing effect, which increased 20.6% of DYP and 12.7% of PP in F1 respectively. Those marker genotypes with increasing effect could be directly used to improve combining ability of restorer lines through the marker- assistant selection.

Key words: Japonica rice, Combining ability, SSR, Yield and yield components, Marker genotypes

[1]Deng H-F(邓华凤), He Q(何强). Status and technical strategy on development of japonica hybrid rice in china. Hybrid Rice (杂交水稻), 2006, 21(1): 1-6 (in Chinese with English abstract)
[2]Yang Z-Y(杨振玉), Chen Q-B(陈秋柏), Chen R-F(陈荣芳). The breeding of japonica rice restorer C57. Acta Agron Sin (作物学报), 1981, 7(8): 153-156 (in Chinese with English abstract)
[3]Yang Z-Y(杨振玉), Chen Q-B(陈秋柏), Chen R-F(陈荣芳). The breeding of japonica hybrid rice Liyou57. Sci Agric Sin (中国农业科学), 1982,15 (1): 38-42 (in Chinese with English abstract)
[4]Lu Z-M(陆作楣). On the combining ability selection in hybrid rice breeding. Chin J Rice Sci (中国水稻科学), 1999, 13(1): 1-5 (in Chinese with English abstract)
[5]Lu Z-M(陆作楣), Tao J(陶瑾). Study of combining ability in hybrid rice of three lines lection and selection of combination. Seed (种子), 1986, (4): 24-28 (in Chinese)
[6]Hong D-L(洪德林), Pan E-F(潘恩飞), Chen C-Q(陈长青). Comparative studies on harvest index between hybrids and pure lines in japonica rice (Oryza sativa 南京农业大学学报), 1998, 21(4): 12-18 (in Chinese with English abstract)L.). J Nanjing Agric Univ (
[7]Hong D-L(洪德林), Yang K-Q(杨开晴), Pan E-F(潘恩飞). Hete-rosis of F1s derived from different ecological types and combining ability of their parents in japonica rice (Oryza sativa L.). Chin J Rice Sci (中国水稻科学), 2002, 16(3): 216-220 (in Chinese with English abstract)
[8]Leng Y(冷燕), Hong D-L(洪德林). Quality trait of hybrid rice grain derived from different ecological type and their genetic analysis in japonica rice (Oryza sativa 中国水稻科学), 2004, 18 (1): 29-33 (in Chinese with English abstract)L.). Chin J Rice Sci (
[9]Li J-H(李建红), Hong D-L(洪德林). Combining ability analysis of main agronomic and quality traits of BT type CMS lines newly bred in japonica rice. J Nanjing Agric Univ (南京农业大学学报), 2004, 27(4): 11-16 (in Chinese with English abstract)
[10]Li J-H(李建红), Hong D-L(洪德林). Combining ability for agronomic and quality traits of BT type iso-cytoplasmic restorer lines newly bred in japonica rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2005, 31(7): 851-857 (in Chinese with English abstract)
[11]Jin W-D(金伟栋), Zhang W(张旺), Hong D-L(洪德林). Heterosis of late-maturing japonica hybrids (Oryza sativa L
[J].) and pa-rent’s combining ability in south Jiangsu region. Acta Agron Sin (作物学报.2005, 31(11):1478-1484
[12]Yuan L(袁玲), Zhu L-L(祝莉莉), He G-C(何光存).Localization of genes controlling rice quality traits with SSR markers
[J].Wuhan Univ J (Nat Sci Edn)(武汉大学学报·理学版.2002, 48(4):507-510
[13]Ni D-H(倪大虎), Yi C-X(易成新), Yang J-B(杨剑波). Developing rice lines resistant to bacterial blight and blast with molecular marker-assisted selection
[J]. Acta Agron Sin (作物学报.2008, 34(1):100-105
[14]Wang Y(王岩), Fu X-M(付新民), Gao G-J(高冠军), He Y-Q(何予卿). Improving the e grain quality of Minghui 63, a restorer line of rice with good quality through marker-assisted selection. Mol Plant Breed (分子植物育种), 2009, 7(4): 661-665 (in Chinese with English abstract)
[15]Liu X C, Wu J L. SSR heterogenic patterns of parents for marking and predicting heterosis in rice breeding
[J].Mol Breed
[16]Liu X C, Ishiki K, Wang W X. Identification of favorable AFLP markers to heterosis in hybrid rice
[J].Breed Sci
[17]Liu X C, Chen S C, Chen J S, Ishiki K, Wang W X, Yu L Q. Improvement of combining ability for restorer lines with identified SSR marker in hybrid rice breeding
[J].Breed Sci
[18]Liu X-C(刘小川), Wang W-X(王渭霞). Identification of molecular markers referred to the grain quality traits of rice hybrids based on the combining ability of their parental lines. Chin J Rice Sci (中国水稻科学), 2005, 19(1): 25-28 (in Chinese with English abstract)
[19]Cheng B-S(程宝山), Wan Z-B(万志兵), Hong D-L(洪德林). Establishment of SSR fingerprint map and analysis of genetic similarity among 35 varieties in japonica rice (Oryza sativa 南京农业大学学报), 2007, 30(3): 1-8 (in Chinese with English abstract)L.). J Nanjing Agric Univ (
[20]Shi K-Y(时宽玉), Hong D-L(洪德林). Polymorphism of 6 hybrid ids and their parents amplified by SSR primers and its application in rice (Oryza sativa L.). J Nanjing Agric Univ (南京农业大学学报), 2005, 28(4): 1-5 (in Chinese with English abstract)
[21]Mu P(穆平), Zhang H-L(张洪亮), Jiang D-F(姜德峰), Liu L-F(刘立峰), Li Z-C(李自超). QTL mapping and interactions between QTL and environment for yield and its components using a DH population derived from a lowland and upland rice Cross. Sci Agric Sin (中国农业科学), 2005, 38(9): 1725-1733 (in Chinese with English abstract)
[22]Ye S-P(叶少平), Zhang Q-J(张启军), Li J-Q(李杰勤), Zhao B(赵兵), Li P(李平). QTL mapping for yield component traits using (Pei’ai 64s/Nipponbare) F2 population. Acta Agron Sin (作物学报), 2005, 31(12): 1620-1627 (in Chinese with English abstract)
[23]Xu J-L(徐建龙), Xue Q-Z(薛庆中), Luo L-J(罗利军), Li Z-K(黎志康). QTL dissection of panicle number per plant and spikelet number per panicle in rice (Oryza sativa L.). Acta Genet Sin (遗传学报), 2001, 28(8): 752-759 (in Chinese with English abstract)
[24]Xing Y-Z(邢永忠), Xu G-C(徐才国), Hua J-P(华金平), Tan Y-F(谈移芳). Analysis of QTL × Environment interaction for rice panicle characteristics. Acta Genet Sin (遗传学报), 2001, 25(5): 439-446 (in Chinese with English abstract)
[25]Xu L(许凌), Zhang Y-D(张亚东), Zhu Z(朱镇), Wang C-L(王才林). Dissection of QTLs in two year s for yield component traits in rice (Oryza sativa L.). Chin J Rice Sci (中国水稻科学), 2008, 22(4): 370-376 (in Chinese with English abstract)
[26]Guo L-B(郭龙彪), Luo L-J(罗利军), Xing Y-Z(邢永忠), Xu G-C(徐才国). Dissection of QTLs in two years for important agronomic traits in rice (Oryza sativa L.). Chin J Rice Sci (中国水稻科学), 2003, 17(3): 211-218 (in Chinese with English abstract)
[27]Ma D-P(马大鹏), Luo L-J(罗利军), Wang C-Y(汪朝阳), He Y-Q(何予卿). Mapping QTLs for yield and its component traits of rice using a recombinant inbred line population. Mol Plant Breed (分子植物育种), 2004, 2(4): 507-512 (in Chinese with English abstract)
[28]Li S-B(李绍波), Yang G-H(杨国华), Zhang Z-H(章志宏), Zhu Y-G(朱英国).Mapping of QTL controlling yield component related traits in rice
[J].Wuhan Univ J (Nat Sci Edn)(武汉大学学报·理学版.2008, 54(6):713-718
[29]Du J-H(杜景红), Fan Y-Y(樊叶杨), Wu J-R(吴季荣), Zhuang J-Y(庄杰云). Dissection of QTLs for yield traits on the short arm of rice chromosome 6. Sci Agric Sin, 2008, 41(4): 939-945 (in Chinese with English abstract)
[30]Mu P(穆平), Huang C(黄超), Li J-X(李君霞), Liu L-F(刘立峰), Liu Y-J(刘弋菊), Li Z-C(李自超). Yield trait variation and QTL mapping in a DH population of rice under phosphorus deficiency. Acta Agron Sin (作物学报), 2008, 34(7): 1137-1142 (in Chinese with English abstract)
[31]Zhao X Q, Xu J L, Zhao M, Lafitte R, Zhu L H, Fu B Y, Li Z K. QTLs affecting morph-physiological traits related to drought tolerance detected in overlapping introgression lines of rice (Oryza sativa L
[J].). Plant Sci
[32]Brondani C, Rangel P, Brondani R, Ferreira M. QTL mapping and introgression of yield related traits from oryza glumaepatula to cultivated rice (Oryza sativa L.) using micro satellite markers. Theor Appl Genet, 2002,104: 1192-1203
[33]Yoon D B, Kang K H, Kim H J, Ju H G, Kwon S J, Suh J P, Jeong Q Y, Ahn S N. Mapping quantitative trait loci for yield components and morphological traits in an advanced backcross population between Oryza grandiglumis and the O. sativa japo- nica cultivar Hwaseongbyeo. Theor Appl Genet, 2006, 112: 1052-1062
[34]Mo H-D(莫惠栋). The analysis of combining ability in p×q mating pattern. J Jiangsu Agric Coll (江苏农学院学报), 1982, 3(3): 51-57 (in Chinese)
[35]Mo H-D(莫惠栋). The analysis of combining ability in p×q mating pattern (continued). J Jiangsu Agric Coll (江苏农学院学报), 1982, 3(4): 53-57 (in Chinese)
[36]Xu C-W(徐辰武), Zhang A-H(张爱红), Zhu Q-S(朱庆森). Genetic analysis of quality characters of rice grain in indica-japo-nica hybrids. Acta Agron Sin (作物学报), 1996, 22(5): 530-534 (in Chinese with English abstract)
[37]Gu F-L(谷福林), Su Z-Q(苏自强), Wang S-F(王水方). 86 You 8, a new fine quality japonica hybrid rice combination. Hybrid Rice (杂交水稻), 2001, 16(2): 59-60 (in Chinese)
[1] CHEN Xiao-Hong, LIN Yuan-Xiang, WANG Qian, DING Min, WANG Hai-Gang, CHEN Ling, GAO Zhi-Jun, WANG Rui-Yun, QIAO Zhi-Jun. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR [J]. Acta Agronomica Sinica, 2022, 48(4): 908-919.
[2] ZHANG Xia, YU Zhuo, JIN Xing-Hong, YU Xiao-Xia, LI Jing-Wei, LI Jia-Qi. Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials [J]. Acta Agronomica Sinica, 2022, 48(4): 920-929.
[3] WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[4] HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
[5] LIU Shao-Rong, YANG Yang, TIAN Hong-Li, YI Hong-Mei, WANG Lu, KANG Ding-Ming, FANG Ya-Ming, REN Jie, JIANG Bin, GE Jian-Rong, CHENG Guang-Lei, WANG Feng-Ge. Genetic diversity analysis of silage corn varieties based on agronomic and quality traits and SSR markers [J]. Acta Agronomica Sinica, 2021, 47(12): 2362-2370.
[6] LI Jing-Lin, LI Jia-Lin, LI Xin-Peng, AN Bao-Guang, ZENG Xiang, WU Yong-Zhong, HUANG Pei-Jing, LONG Tuan. Creation and combining ability analysis of recessive genic sterile lines with a new ptc1 locus in rice [J]. Acta Agronomica Sinica, 2021, 47(11): 2173-2183.
[7] HUANG Heng, JIANG Heng-Xin, LIU Guang-Ming, YUAN Jia-Qi, WANG Yuan, ZHAO Can, WANG Wei-Ling, HUO Zhong-Yang, XU Ke, DAI Qi-Gen, ZHANG Hong-Cheng, LI De-Jian, LIU Guo-Lin. Effects of side deep placement of nitrogen on rice yield and nitrogen use efficiency [J]. Acta Agronomica Sinica, 2021, 47(11): 2232-2249.
[8] GUO Yan-Chun, ZHANG Li-Lan, CHEN Si-Yuan, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Lie-Mei, ZHANG Li-Wu. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2021, 47(1): 80-93.
[9] WANG Heng-Bo,QI Shu-Ting,CHEN Shu-Qi,GUO Jin-Long,QUE You-Xiong. Development and application of SSR loci in monoploid reference genome of sugarcane cultivar [J]. Acta Agronomica Sinica, 2020, 46(4): 631-642.
[10] Hong-Yan ZHANG,Tao YANG,Rong LIU,Fang JIN,Li-Ke ZHANG,Hai-Tian YU,Jin-Guo HU,Feng YANG,Dong WANG,Yu-Hua HE,Xu-Xiao ZONG. Assessment of genetic diversity by using EST-SSR markers in Lupinus [J]. Acta Agronomica Sinica, 2020, 46(3): 330-340.
[11] Li-Lan ZHANG, Lie-Mei ZHANG, Huan-Ying NIU, Yi XU, Yu LI, Jian-Min QI, Ai-Fen TAO, Ping-Ping FANG, Li-Wu ZHANG. Correlation between SSR markers and fiber yield related traits in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2020, 46(12): 1905-1913.
[12] LIU Rong, WANG Fang, FANG Li, YANG Tao, ZHANG Hong-Yan, HUANG Yu-Ning, WANG Dong, JI Yi-Shan, XU Dong-Xu, LI Guan, GUO Rui-Jun, ZONG Xu-Xiao. An integrated high-density SSR genetic linkage map from two F2 population in Chinese pea [J]. Acta Agronomica Sinica, 2020, 46(10): 1496-1506.
[13] XIANG Li-Yuan,XU Kai,SU Jing,WU Chao,YUAN Xiong,ZHENG Xing-Fei,DIAO Ying,HU Zhong-Li,LI Lan-Zhi. Genetic dissection of combining ability and heterosis of rice agronomic traits based on pathway analysis [J]. Acta Agronomica Sinica, 2019, 45(9): 1319-1326.
[14] YE Wei-Jun,CHEN Sheng-Nan,YANG Yong,ZHANG Li-Ya,TIAN Dong-Feng,ZHANG Lei,ZHOU Bin. Development of SSR markers and genetic diversity analysis in mung bean [J]. Acta Agronomica Sinica, 2019, 45(8): 1176-1188.
[15] Zhi-Jun TONG,Yi-Han ZHANG,Xue-Jun CHEN,Jian-Min ZENG,Dun-Huang FANG,Bing-Guang XIAO. Mapping of quantitative trait loci conferring resistance to brown spot in cigar tobacco cultivar Beinhart1000-1 [J]. Acta Agronomica Sinica, 2019, 45(3): 477-482.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!