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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1270-1279.doi: 10.3724/SP.J.1006.2010.01270


Screening Marker Genotypes with Elite Combining Ability for Yield Traits in Parents of Hybrid Japonica Rice (Oryza sativa L.)

LIANG Kui1, HUANG Dian-Cheng1,2,**, ZHAO Kai-Ming1, NGUYEN Phuong-Tung1,3,XIE Hui1,4,MA Wen-Xia,HONG De-Lin1,*   

  1. 1State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China;2Cotton Research Institute,chinese Academy of Agricultural Sciences,Anyang 455000,China;3Agricultural Science Institute of Northem Central Vietnam,Nghe An-Vinh,Vietnam;4Tianjin Subcentre of china National Hybrid Rice research and development Center,Tianjin 300457,China
  • Received:2010-02-01 Revised:2010-04-19 Online:2010-08-12 Published:2010-05-20
  • Contact: HONG De-LIn, E-mail: delinhong@njau.edu.cn, Tel: 025-84396626

Abstract: The area of japonica rice planted annually is 8 280 000 ha and accounts for 27% of total rice area in China. The area planting japonica hybrid rice is only 3% of the total area of japonica rice. The major reason for this situation is that competitive heterosis of hybrid cultivar is not conspicuous in yield, compared with conventional cultivar in japonica rice. The key factor of enhancing competitive heterosis of hybrid cultivar in japonica rice is to improve combining ability of yield related traits of restorer lines. In order to improve combining ability of restorer lines more efficiently, SSR marker genotypes with elite combining ability for five traits were screened in this study, by analyzing the data of combining ability and SSR markers in six CMS lines and twelve restorer lines that were genotyped using 115 pairs of SSR primers. Combining ability of the 18 parental lines was analyzed for daily yield per plant (DYP), panicles per plant (PP), total spikelets per panicle (TSP), filled spikelets per panicle (FSP) and 1000-grain weight (1000-GW), using the data of 72 F1s made with NC II genetic design. Results showed that twenty SSR marker genotypes were significantly associated with combining ability of the five traits. Among them, eight for only one trait, six for two traits, four for three traits and two for four traits. Marker genotypes associated with combining ability of multiple traits increased or decreased trait value of F1. RM152-165/170 was a marker genotype of elite combining ability for PP and DYP with the maximum increasing effect, which increased 20.6% of DYP and 12.7% of PP in F1 respectively. Those marker genotypes with increasing effect could be directly used to improve combining ability of restorer lines through the marker- assistant selection.

Key words: Japonica rice, Combining ability, SSR, Yield and yield components, Marker genotypes

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