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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1265-1269.doi: 10.3724/SP.J.1006.2010.01265


Molecular Mapping of Leaf Rust Resistance Genes in Wheat Line Tian 95HF2

ZHOU Yue,WANG Long,ZHANG Ye,LI Zai-Feng*,LIU Da-Qun*   

  1. College of Plant Protection, Agricultural University of Hebei / Biological Control Center for Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China
  • Received:2010-03-15 Revised:2010-04-24 Online:2010-08-12 Published:2010-06-11
  • Contact: LIU Da-Qun, E-mail: ldq@hebau.edu.cn, Tel: 0312-7528500;LI Zai-Feng, E-mail: lzf7551@yahoo.com.cn, Tel: 0312-7528500

Abstract: The wheat (Triticum aestivum L.) line Tian 95HF2 appears in low infection to most of Chinese current pathotypes of Puccinia triticina at seedling stage. For postulating the resistance genes in Tian 95HF2, F1 and F2 populations from the cross between Tian 95HF2 and Zhengzhou 5389 (susceptible to leaf rust) were inoculated with pathotypes FHTT and PHTS in greenhouse. The infection types were investigated 15 d after inoculation and molecular markers were also used for mapping the resistance genes. When inoculating with pathotype FHTT, only one resistance gene was detected. After screening 1274 primer pairs in the parents and the resistant and susceptible bulks, the resistance gene was found between the interval of a sequence tag site for Lr1 (WR003) and a microsatellite (wmc443) on 5DL.The genetic distances to the two markers were 2.9 cM and 3.1 cM, respectively. This resistance gene was finally postulated to be Lr1. The segregation of infection type in response to pathotype PHTS showed that there were two resistance genes in Tian 95HF2. One was inferred as Lr1, and the other was likely to be LrZH84, which is located on chromosome 1BL.

Key words: SSR marker, Resistance gene to wheat leaf rust, Tian 95HF2, Lrl, LrZH84

[1]Denissen C J M. Influence of race and post infection temperature on two components of partial resistance to wheat leaf in seedlings of wheat
[2]Komer J A. Genetics of resistance to wheat leaf rust
[J].Annu Rev Phytopathol
[3]Dong J-G(董金皋). Plant Pathology in Agriculture (农业植物病理学). Beijing: China Agriculture Press, 2001. pp 44-45 (in Chinese)
[4]McIntosh R A, Dubcovsky J, Rogers W J, Morris C, Appels R, Xia X C. Catalogue of gene symbols for wheat: 2009 supplement.
[2010-03-05]. http://www.shigen.nig.ac.jp/wheat/komugi/genes/ macgene/supplement2009.pdf
[5]Watson I A, Singh D. The future for rust resistant wheat in Australia. J Aust Inst Agric Sci, 1952, 18: 190-197
[6]Eriksen L, Afshari F, Christiansen M J, Mclntosh R A, Jahoor A, Wellings C R. Yr32 for resistance to stripe (yellow) rust present in the wheat cultivar Carstens V
[J].Theor Appl Genet
[7]McIntosh R A, Lagudah E S. Cytogenetical studies in wheat: XVIII. Gene Yr24 for resistance to stripe rust. Plant Breed, 2000, 119: 81-93
[8]Wan A M, Zhao Z H, Chen X M, He Z G, Jin S H, Jia Q Z, Yao G, Yang J X, Wang B T, Li G B, Bi Y Q, Yuan Z Y. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Dis, 2004, 88: 896-904
[9]Yuan J-H(袁军海), Liu T-G(刘太国), Chen W-Q(陈万全). Postulation of leaf rust resistance genes in 47 new wheat cultivars at seedling stage. Sci Agric Sin (中国农业科学), 2007, 40(9): 1925-1935 (in Chinese with English abstract)
[10]Li Z F, Xia X C, He Z H, Li X, Zhang L J, Wang H Y, Meng Q F, Yang W X, Li G Q, Liu D Q. Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars
[J].Plant Dis
[11]Sacco F, Suarez E Y, Narango T. Mapping of the leaf rust resistance gene Lr3 on chromosome 6B of Sinvalocho MA wheat. Genome, 1998, 41: 686-690
[12]Yang W-X(杨文香), Liu D-Q(刘大群). Advances in localization and molecular markers of wheat leaf rust resistance genes. Sci Agric Sin (中国农业科学), 2004, 37(1): 65-71 (in Chinese with English abstract)
[13]Khan R R, Bariana H S, Dholakia B B, Naik S V, Lagu M D, Rathjen A J, Bhavani S, Gupta V S. Molecular mapping of stem and leaf rust resistance in wheat
[J].Theor Appl Genet
[14]Helguera M, Vanzetti L, Soria M, Khan I A, Kolmer J, Dubcovsky J. PCR markers for Triticum speltoides leaf rust resistance gene Lr51 and their use to develop isogenic hard red spring wheat lines
[J]. Crop Sci
[15]Hiebert C, Thomas J, McCallum B. Locating the broad-spectrum wheat leaf rust resistance gene Lr52 (LrW) to chromosome 5B by a new cytogenetic method
[J].Theor Appl Genet
[16]McIntosh R A, Devos K M, Dubcovsky J, Rogers W J, Morris C F, Appels R, Somers D J, Anderson O A. Catalogue of gene symbols for wheat: 2007 supplement. Annu Wheat Newsl, 2007, 53: 159-180
[17]McIntosh R A, Devos K M, Dubcovsky J, Rogers W J, Morris C F, Appels R, Somers D J, Anderson O A. Catalogue of gene symbols for wheat: 2008 Supplement. Annu Wheat Newsl, 2008, 54: 209-225
[18]Li Z F, Zheng T C, He Z H, Li G Q, Xu S C, Li X P, Yang G Y, Singh R P, Xia X C. Molecular tagging of stripe rust resistance gene YrZH84 in Chinese wheat line Zhou 8425B
[J]. Theor Appl Genet
[19]Zhao X L, Zheng T C, Xia X C, He Z H, Liu D Q, Yang W X, Yin G H, Li Z F. Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B
[J].Theor Appl Genet
[20]Long D L, Kolmer J A. A North American system of nomenclature for Puccinia triticina
[21]Dubin H J, Johnson R, Stubbs R W. Postulated genes for resistance to strip rust in selected CIMMYT and related wheats
[J].Plant Dis
[22]Sharp P J, Kreis M, Shewry P R, Gale M D. Location of β-amylase sequence in wheat and its relatives
[J].Theor Appl Genet
[23]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations
[J].Proc Natl Acad Sci USA
[24]Chai J F, Zhou R H, Jia J Z, Liu X. Development and application of a new codominant PCR marker for detecting 1BL·1RS wheat-rye chromosome translocations
[J].Plant Breed
[25]Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P, Ganal M W. A microsatellite map of wheat. Genetics, 1998, 149: 2007-2023
[26]Qiu J W, Schürch A C, Yahiaoui N, Dong L L, Fan H J, Zhang Z J, Keller B, Ling H Q. Physical mapping and identification of a candidate for the leaf rust resistance gene Lr1 of wheat
[J].Theor Appl Genet
[27]Li Z F, Xia X C, Zhou X C, Niu Y C, He Z H, Zhang Y, Li G Q, Wan A M, Wang D S, Chen X M, Liu Q L, Singh R P. Seeding and slow rusting resistance to stripe rust in Chinese common wheats
[J].Plant Dis
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