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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (10): 1565-1572.doi: 10.3724/SP.J.1006.2017.01565

• RESEARCH NOTES • Previous Articles    

Rapid Identification System of Purity and Authenticity in Cotton Varieties By SSR Markers

WANG Xin-Yi1,AI Xian-Tao2,WANG Jun-Duo2,LIANG Ya-Jun2,GONG Zhao-Long2,ZHENG Ju-Yun2,Guo Jiang-Ping2,MAMAT Mo-Ming2,LI Xue-Yuan2,*   

  1. 1 Key Laboratory of Agriculture Biological Technology / College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China; 2 Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
  • Received:2016-12-20 Revised:2017-05-10 Online:2017-10-12 Published:2017-05-19
  • Contact: 李雪源, E-mail: xjmh2338@163.com E-mail:15999173011@163.com
  • Supported by:

    The work was supported by the National Natural Science Foundation of China (31360351).

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Abstract:

SSR molecular markers were used to analyze the authenticity and purity of cotton varieties, providing a molecular basis for the identification of cotton varieties. Typical cotton varieties were used as materials, and 26 pairs of primers well-distributed on the cotton genome were ascertained as the core primers in SSR molecular identification by preliminary and multiple screening. A total of 138 alleles from 120 varieties were screened by using 26 pairs of core primers, 129 of which were polymorphic loci, with a polymorphism rate of 93.48%. Ten inbred cotton varieties were used with 26 pairs of SSR core primers to identify the variety purity. The DNA fingerprint of one variety (Yuanmian 6) shared high uniformity with that of the standard cultivar, and DNA fingerprints of other nine varieties had 8–18 loci different from those of standard cultivar. The results from SSR molecular marker matched with those from field experiments. Five specific primers were selected for testing authenticity of each cultivar from 26 core primers. The variety with highest purity was Yuanmian 6, with the purity of 98.0% and that with lowest purity was Yuanmian 1, with the purity of 90.5%. It suggests that identifying authenticity and purity of cotton varieties is feasible by SSR molecular markers.

Key words: Cotton, Authenticity identification, Purity testing, SSR marker

[1] Ashok B D, Mamta P R, Manoj R B, Kshanada J M. Identification and genetic purity testing of cotton F1 Hybrid using molecular markers. Indian J Biotechnol, 2011, 10: 301–306
[2] 刘峰, 冯雪梅, 钟文, 刘玉栋, 阴祖军, 韩秀兰, 徐子初, 沈法富. 适合棉花品种鉴定的SSR核心引物的筛选. 分子植物育种, 2009, 7: 1160–1168
Liu F, Feng X M, Zhong W, Liu Y D, Yin Z J, Han X L, Xu Z C, Sheng F F. Screening of SSR core primer pairs for identificating cotton cultivar. Mol Plant Breed, 2009, 7: 1160–1168 (in Chinese with English abstract)
[3] 周大云, 杨伟华, 魏守军, 王延琴, 匡猛, 马磊, 方丹, 侯爱玲. 棉种纯度和真实性3种鉴定方法简介. 中国棉花, 2015, 42(8): 5–7
Zhou D Y, Yang W H, Wei S J, Wang Y Q, Kuang M, Ma L, Fang D, Hou A L. A brief introduction of 3 methods for identification of purity and authenticity of cotton seed. China Cotton, 2015, 42(8): 5–7 (in Chinese)
[4] 武耀廷, 张天真, 郭旺珍, 殷剑美. 陆地棉品种SSR标记的多态性及用于杂交种纯度检测的研究. 棉花学报, 2001, 13: 131–133
Wu Y T, Zhang T Z, Guo W Z, Yin J M. Detecting polymorphism among upland cotton (Gossypium hirsutum L.) cultivars and their roles in seed purity of hybrids with SSR markers. Cotton Sci, 2001, 13: 131–133 (in Chinese with English abstract)
[5] 朱美霞, 李英芝, 王建书, 侯英梅, 孟艳玲. 利用SSR方法鉴定棉花品种纯度. 安徽农业科学, 2005, 33: 2010–2016
Zhu M X, Li Y Z, Wang J S, Hou Y M, Meng Y L. Purity identification of cotton varieties with SSR technique. J Anhui Agric Sci, 2005, 33: 2010–2016 (in Chinese with English abstract)
[6] 殷剑美, 陈旭升, 狄佳春, 许乃银, 肖松华, 刘剑光, 吴巧娟. 杂交棉苏杂118的SSR指纹图谱构建. 江苏农业科学, 2007, (4): 29–31
Yin J M, Chen X S, Di J C, Xu N Y, Xiao S H, Liu J G, Wu Q J. Establishment of finger printing of SSR mark for Suza 118 hybrid. Jiangsu Agric Sci, 2007, (4): 29–31 (in Chinese)
[7] Yu J Z, Fang D D, Kohel R J, Ulloa M, Hinze L L, Percy R G, Zhang J F, Chee P, Scheffler B E, Jones D C. Development of a core set of SSR markers for the characterization of Gossypium germplasm. Euphytica, 2012, 187: 203–213
[8] 刘国栋, 王芙蓉, 宫永超, 马和欢, 张军. 棉花品种遗传纯度的SSR分子标记鉴定技术研究. 棉花学报, 2013, 25: 382–387
Liu G D, Wang F R, Gong Y C, Ma H H, Zhang J. A new method for identification of the genetic purity of cotton varieties by SSR markers. Cotton Sci, 2013, 25: 382–387 (in Chinese with English abstract)
[9] 白静. 杂交棉品种SSR核心引物的筛选与真实性和纯度鉴定. 华中农业大学硕士学位论, 湖北武汉, 2011
Bai J. Screening of SSR Core Primer and the Identification of Genuineness and Purity on Hybrid Cotton. MS Thesis of Huazhong Agricultural University, Wuhan, China, 2011 (in Chinese with English abstract)
[10] Porebski S, Bailey L G, Baum B R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep, 1997, 15: 8–15
[11] 张小娟, 陆徐忠, 何团结, 路曦结, 郑曙峰, 倪金龙, 张冰清, 杨剑波. 利用SSR分子标记进行棉花品种鉴定的研究. 棉花学报, 2014, 26: 483–491
Zhang X J, Lu X Z, He T J, Lu X J, Zheng S F, Ni J L, Zhang B Q, Yang J B. Identification of cotton varieties using SSR molecular markers. Cotton Science, 2014, 26: 483–491 (in Chinese with English abstract)
[12] 聂新辉, 尤春源, 李晓方, 秦江鸿, 黄聪, 郭欢乐, 王夏青, 赵文霞, 林忠旭. 新陆早棉花品种DNA指纹图谱的构建及遗传多样性分析. 作物学报, 2014, 40: 2104–2117
Nie X H, You C Y, Li X F, Qin J H, Huang C, Guo H L, Wang X Q, Zhao W X, Lin Z X. Construction of DNA Fingerprinting and Analysis of Genetic Diversity for Xinluzao Cotton Varieties. Acta Agron Sin, 2014, 40: 2104–2117
[13] 艾先涛, 梁亚军, 沙红, 王俊铎, 郑巨云, 吐尔逊江, 多力坤, 李雪源, 华金平. 新疆自育陆地棉品种SSR 遗传多样性分析. 作物学报, 2014, 40: 369–379
Ai X T, Liang Y J, Sha H, Wang J D, Zheng J Y, Tu X J, Duo L K, Li X Y, Hua J P. Genetic diversity analysis on local upland cotton cultivars in Xinjiang based on SSR markers. Acta Agron Sin, 2014, 40: 369–379 (in Chinese with English abstract)
[14] 张军, 武耀廷, 郭旺珍, 张天真. 棉花微卫星标记的PAGE/银染的快速检测. 棉花学报, 2000, 12: 267–269
Zhang J, Wu Y T, Guo W Z, Zhang T Z. Fast screening of microsatellite markers in cotton with PAGE/ silver staining. Cotton Sci, 2000, 12: 267–269 (in Chinese with English abstract)
[15] Li L, Yang J B, Wang X F, Xiang T H, Wu J D. Identification of Xieyou combinations of hybrid rice and their three parents by using RAPD and microsatellite markers. Chin J Rice Sci, 2000, 14: 203–207
[16] Yao Q L, Yang K C, Pan G T. Genetic diversity of maize (Zea mays L.) landraces from Southwest China based on SSR data. J Genet Genom, 2007, 34: 851–860
[17] 王立新, 李云伏, 常利芳, 黄岚, 李宏博, 葛玲玲, 刘丽华, 姚骥, 赵昌平. 建立小麦品种DNA指纹的方法研究. 作物学报, 2007, 33: 1738–1740
Wang L X, Li Y F, Chang L F, Huang L, Li H B, Ge L L, Liu L H, Yao J, Zhao C P. Method of ID constitution for wheat cultivars. Acta Agron Sin, 2007, 33: 1738–1740 (in Chinese with English abstract)
[18] 王俊芳. 基于SSR标记的棉种真实性和品种纯度鉴定技术研究. 中国农业科学院硕士学位论文, 北京, 2009
Wang J F. SSR Markers-Based Cotton Purity and Authenticity Identification. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing, China, 2009 (in Chinese with English abstract)
[19] 计怀春, 孙君灵, 周中立, 潘兆娥, 贾银华, 何守朴, 庞保印, 王立如, 杜雄明. 陆地棉繁殖群体大小对遗传完整性的影响. 棉花学报, 2014, 26: 145–152
Ji H C, Sun J L, Zhou Z L, Pan Z E, Jia Y H, He S P, Pang B Y, Wang L R, Du X M. Effects of population size on seed increase for genetic integrity of cotton. Cotton Sci, 2014, 26: 145–152 (in Chinese with English abstract)
[20] 刘文献, 李立会, 刘伟华, 张正茂, 吴振海, 王成社. 华山新麦草居群取样策略的SSR分析. 麦类作物学报, 2006, 26: 16–20
Liu W X, Li L H, Liu W H, Zhang Z M, Wu Z H, Wang C S. SSR analysis on the sampling strategy of psathyrostachys Huashanic keng population. J Triticeae Crops, 2006, 26: 16–20
[21] 匡猛, 杨伟华, 张玉翠, 许红霞, 王延琴, 周大云, 冯新爱, 苏畅, 周红. 棉花杂交种纯度的SSR标记检测及其与田间表型鉴定的相关性. 作物学报, 2011, 37: 2295–2305
Kuang M, Yang W H, Zhang Y C, Xu H X, Wang Y Q, Zhou D Y, Feng X A, Su C, Zhou H. Cotton hybrid purity tested by SSR markers and its correlation with phenotype identification in field. Acat Agron Sin, 2011, 37: 2295–2305 (in Chinese with English abstract)
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