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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (10): 1565-1572.doi: 10.3724/SP.J.1006.2017.01565

• RESEARCH NOTES • Previous Articles    

Rapid Identification System of Purity and Authenticity in Cotton Varieties By SSR Markers

WANG Xin-Yi1,AI Xian-Tao2,WANG Jun-Duo2,LIANG Ya-Jun2,GONG Zhao-Long2,ZHENG Ju-Yun2,Guo Jiang-Ping2,MAMAT Mo-Ming2,LI Xue-Yuan2,*   

  1. 1 Key Laboratory of Agriculture Biological Technology / College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China; 2 Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
  • Received:2016-12-20 Revised:2017-05-10 Online:2017-10-12 Published:2017-05-19
  • Contact: 李雪源, E-mail: xjmh2338@163.com E-mail:15999173011@163.com
  • Supported by:

    The work was supported by the National Natural Science Foundation of China (31360351).

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Abstract:

SSR molecular markers were used to analyze the authenticity and purity of cotton varieties, providing a molecular basis for the identification of cotton varieties. Typical cotton varieties were used as materials, and 26 pairs of primers well-distributed on the cotton genome were ascertained as the core primers in SSR molecular identification by preliminary and multiple screening. A total of 138 alleles from 120 varieties were screened by using 26 pairs of core primers, 129 of which were polymorphic loci, with a polymorphism rate of 93.48%. Ten inbred cotton varieties were used with 26 pairs of SSR core primers to identify the variety purity. The DNA fingerprint of one variety (Yuanmian 6) shared high uniformity with that of the standard cultivar, and DNA fingerprints of other nine varieties had 8–18 loci different from those of standard cultivar. The results from SSR molecular marker matched with those from field experiments. Five specific primers were selected for testing authenticity of each cultivar from 26 core primers. The variety with highest purity was Yuanmian 6, with the purity of 98.0% and that with lowest purity was Yuanmian 1, with the purity of 90.5%. It suggests that identifying authenticity and purity of cotton varieties is feasible by SSR molecular markers.

Key words: Cotton, Authenticity identification, Purity testing, SSR marker

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