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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (07): 1039-1046.doi: 10.3724/SP.J.1006.2015.01039


Fine Mapping and Map Integration of Brsc1 Gene in Dahuang Rape (Brassica rapa L.)

LI Xin**,XIAO Lu**,DU De-Zhi*   

  1. Province for Spring Rapeseed Genetic Improvement / National Key Laboratory Breeding Base of Qinghai Province for Innovation and Utilization of Plateau Crop Germplasm, Xining 810016, China
  • Received:2014-12-04 Revised:2015-04-02 Online:2015-07-12 Published:2015-04-14
  • Contact: 杜德志, E-mail: qhurape@126.com, Tel: 0971-5366520 E-mail:1052719375@qq.com


Dahuang rape, a landrace originated from Qinghai Huangyuan, has bright yellow seed coat. Previous studies indicated that the yellow-seeded trait in Dahuang was controlled by a recessive gene (Brsc1), which was located in a 1.7 Mb interval on chromosome A9 of Brassica rapa (B. rapa). In order to better use the yellow-seeded resource, we further fine mapped the Brsc1 gene. BC4 and F2 populations, constructed from the cross of Dahuang and 09A-126 (brown-seeded, B. rapa), were used for fine mapping. New PCR markers were developed based on the information of the homologous region in B. rapa and the published SSR markers were used in polymorphism survey. A total of six markers (BrID10711, BrA5-BrA9) tightly linked to the target gene were obtained, wherein BrA5 co-segregated with Brsc1, and BrA9 was 0.69 cM away from the Brsc1 gene. So Brsc1 was further limited in a 1.2 Mb interval, approximately. The markers identified in this study were used to detect three types of individual plants in F2 population. BrA8 was identified as a co-dominant marker. Marker density of the region encompassing the Brsc1 gene was increased by integrating the markers from previous researches and this study. Sequence alignment with the genome of Arabidopsis thaliana was performed and a good homologous linear relationship between five markers and chromosome 1 of Arabidopsis thaliana was detected, which implied that homologous gene of the Brsc1 gene maybe located in the chromosome 1 of Arabidopsis thaliana. Markers obtained from this study would provide favorable conditions for map-based cloning of the Brsc1 and molecular marker-assisted selection (MAS) breeding of the yellow-seeded rape.

Key words: Brassica rapa L., Yellow-seeded, SSR marker, Genetic map, Physical map

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