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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (04): 754-759.doi: 10.3724/SP.J.1006.2012.00754

• RESEARCH NOTES • Previous Articles    

Karyotype Analysis of Arachis hypogaea L. Using Fluorescence Banding and Fluorescence in situ Hybridization with rDNA Probes

SHE Chao-Wen1,2,3,ZHANG Li-Hua1,JIANG Xiang-Hui1,2,3   

  1. 1 Department of Life Sciences, Huaihua University, Huaihua 418008, China; 2 Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua 418008, China; 3 Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education, Huaihua 418008, China
  • Received:2011-08-04 Revised:2011-12-19 Online:2012-04-12 Published:2012-02-13

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Abstract: The establishment of an exact and detailed karyotype of Arachis hypogaea L. was fundamental for clarification of the origin and research of the genome of the species. In this study, the mitotic metaphase chromosomes of the species were analyzed using DAPI banding and double fluorescence in situ hybridization (FISH) with 5S and 45S rDNA probes. The mean haploid karyotype length was (81.06±3.74) μm, the longest chromosome pair was (4.72±0.15) μm and the shortest chromosome pair was (2.62±0.14)μm. In the complements of the species, fifteen pairs of the chromosomes displayed centromeric DAPI+ bands including ten pairs of strong bands and five pairs of weak bands; and two pairs of 5S and five pairs of 45S rDNA sites were showed, with one 5S site being syntenic to a 45S site. Combining the chromosome measurements with DAPI+ bands and rDNA FISH signals, the chromosomes were exactly paired and arranged, and a detailed molecular cytogenetic karyotype of A. hypogaea is established. The karyotype formula of A. hypogaea was 2n=4x=40=38m+2sm (SAT) and the asymmetric karyotype belonged to 2A type.

Key words: Arachis hypogaea, Karyotype, Fluorochrome banding, rDNA, Fluorescence in situ hybridization

[1]Smartt J, Gregory W C, Gregory M P. The genomes of Arachis hypogaea: 1. Cytogenetic studies of putative genome donors. Euphytica, 1978, 27: 665–675

[2]Singh A K, Smartt J. The genome donors of the groundnut/peanut (Arachis hypogaea L.) revisited. Genet Resour Crop Evol, 1998, 45: 113–118

[3]Seijo J G, Lavia G I, Fernández A, Krapovickas A, Ducasse D, Moscone E A. Physical mapping of 5S and 18S–25S rRNA genes by FISH as evidences that Arachis duranensis and A. ipaensis are the wild diploid species involved in the origin of A. hypogaea (Leguminosae). Am J Bot, 2004, 91: 1294–1303

[4]Seijo J G, Lavia G I, Fernández A, Krapovickas A, Ducasse D A, Bertioli D J, Moscone E A. Genomic relationships between the cultivated peanut (Arachis hypogaea–Leguminosae) and its close relatives revealed by double GISH. Am J Bot, 2007, 94: 1963–1971

[5]Robledo G, Lavia G I, Seijo G. Species relations among wild Arachis species with the A genome as revealed by FISH mapping of rDNA loci and heterochromatin detection. Theor Appl Genet, 2009, 118: 1295–1307

[6]Robledo G, Seijo G. Species relationships among the wild B genome of Arachis species (section Arachis) based on FISH mapping of rDNA loci and heterochromatin detection: a new proposal for genome arrangement. Theor Appl Genet, 2010, 121: 1033–1046

[7]Husted L. Cytological studies on the peanut, Arachis. I. Chromosome number and morphology. Cytologia, 1933, 5: 109–117

[8]Husted L. Cytological studies on the peanut, Arachis. II. Chromosome number, morphology and their application to the problem of the origin of the cultivated forms. Cytologia, 1936, 7: 396–423

[9]D'Cruz R, Tankasale M P. A note on chromosome complement of four groundnut varieties. Indian Oilseeds J, 1961, 5: 58–59

[10]Singh A K, Moss J P. Utilization of wild relatives in genetic improvement of Arachis hypogaea L. Part 2: Chromosome complements of species in section Arachis. Theor Appl Genet, 1982, 61: 305–314

[11]Stalker H T, Dalmacio R D. Karyotype analysis and relationships among varieties of Arachis hypogaea L. Cytologia, 1986, 51: 617–629

[12]Zhan Y-X(詹英贤), Wu A-Z(吴爱忠), Cheng M(程明). Karyotype analysis in peanut (Arachis hypogaea L.) of four types. Sci Agric Sin (中国农业科学), 1988, 21(1): 61–67(in Chinese with English abstract)

[13]Wang J-B(王建波), Li R-Q(利容千), Zeng Z-S(曾子申). Karyotype studies on three species in Arachis. Acta Agron Sin (作物学报), 1988, 14(4): 284–289 (in Chinese with English abstract)

[14]Lavia G I, Aveliano F. Karyotypic studies in Arachis hypogaea L. varieties. Caryologia, 2004, 57: 353–359

[15]Sumner A T. Chromosome banding. London: Unwin Hyman, 1990

[16]She C-W(佘朝文), Song Y-C(宋运淳). Progress of plant FISH technique and its applications in the analysis of plant genome. J Wuhan Bot Res (武汉植物学研究), 2006, 24(4): 365–376 (in Chinese with English abstract)

[17]Raina S N, Mukai Y. Detection of a variable number of 18S-5, 8S-26S and 5S ribosomal DNA loci by fluorescent in situ hybridization in diploid and tetraploid Arachis species. Genome, 1999, 42: 52–59

[18]Song Y C, Liu L H, Ding Y, Tian X B, Yao Q, Meng L, He C R, Xu M S. Comparisons of G-banding patterns in six species of the Poaceae. Hereditas, 1994, 121: 31–38

[19]She C W, Liu J Y, Song Y C. CPD staining: an effective technique for detection of NORs and other GC-rich chromosomal regions in plants. Biotech Histochem, 2006, 81: 13–21

[20]Li M-X(李懋学), Chen R-Y(陈瑞阳). A suggestion on the standardization of karyotype analysis in plants. J Wuhan Bot Res (武汉植物学研究), 1985, 3(4): 297–302 (in Chinese with English abstract)

[21]Stebbins G L. Chromosomal Evolution in Higher Plants. London: Edward Arnold Publ. Ltd., 1971

[22]Krapovickas A, Gregory W C. Taxonomía del género Arachis (Leguminosae). Bonplandia, 1994, 8: 1–186
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