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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (08): 1513-1519.doi: 10.3724/SP.J.1006.2014.01513

• RESEARCH NOTES • Previous Articles    

Double Fertilization and Duration of Phases in Peanut (Arachis hgpogaea L.)

SHI Chun-Yan,SHEN Jia-Heng*,LI Wei   

  1. College of Life Science and Technology, Harbin Normal University, Harbin 150080, China
  • Received:2013-12-02 Revised:2014-04-16 Online:2014-08-12 Published:2014-05-16
  • Contact: 申家恒, E-mail: hsdshenjiaheng@163.com, Tel: 0535-6685930

Abstract:

 We used paraffin section technology to observe fertilization and duration of phased in fertilization of Arachis hypogaea L. The results indicated that reproductive cell in the pollen came into mitosis tube one hour after bloom, the mitosis lasted about three hours. Sperms released to embryonary sac at about eight hours after flowering. Sperm and egg cell or polar nucleus or secondary nucleus fused from nine to fifteen hours after flowering. Fusion needed about four hours. There were obvious isometrical two nuclei in the zygote at 30 hours after the two nucleole fusion, which came into division stage. Most of zygote had transverse division, and a few longitudinal division. First division needed about 36 hours after bloom, then formed two-cell proembryo and zygote had a dormant period for about 19 hours. After polar nucleus or secondary nucleus fertilization, almost the fusion completed in 13 hours after bloom, and male nucleolus appeared in one of polar nucleus or secondary nucleus, forming primary endosperm nucleus. The primary endodperm nucleus started division without domancy or after short dormant period in 15 hours after bloom. There were more sperms coming into embryonary sat and also single fertilization phenomenon.This study provides a understanding for morphological changed and corresponding timing of male and female cells in peanut fertiligation, confirms the duration of zygote dormancy, and enriches thepeanut embryological information, which has great significance for peanut breeding and genetically modified (gm) operation.

Key words: Arachis hypogaea L., Fertilization, Zygocyte, Zygote dormancy, Primary endosperm nucleus

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