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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (07): 1094-1099.doi: 10.3724/SP.J.1006.2016.01094

• RESEARCH NOTES • Previous Articles    

Cloning and Functional Analysis of Peanut AhDGAT2a Promoter?

ZHENG Ling1,2,SHI Ling-Min1,3,TIAN Hai-Ying1,2,SHAN Lei1,2,BIAN Fei1,GUO Feng1, XUAN Ning1,WAN Shuo-Bo1,2,*,PENG Zhen-Ying1,2,3,*   

  1. 1 Biotechnology Research Center, Shandong Academy of Agricultural Sciences / Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, China; 2 College of Life Science, Shandong University, Jinan 250100, China; 3 College of Agronomy, Xinjiang Agricultural University, Urumqi 830000, China
  • Received:2016-01-11 Revised:2016-05-09 Online:2016-07-12 Published:2016-05-11
  • Contact: 万书波, E-mail: wansb@saas.ac.cn, Tel: 0531-83178335; 彭振英, E-mail: pengzhenying2005@126.com
  • Supported by:

    This study was supported by Natural Science Foundation of Shandong (ZR2013CM036) and Shandong Province Germplasm Innovation and Utilization Project (2014-2017).

Abstract:

Diacylglycerol acyltransferase (DGAT) is a rate-limiting enzyme in triacylglycerol (TAG) biosynthesis pathway. In this study, GenomeWalking method was used for cloning the promoter sequence of AhDGAT2a gene from Luhua 14, and finally a 1200 bp fragment flanking 5′-upstream of AhDGAT2a was obtained and named as pAhDGAT2a. The crucial regulatory elements in pAhDGAT2a were further analyzed with software PlantCARE. There were many TATA-box, CAAT-box, light regulation, stress and defense response and hormone response elements. To assess the activity of pAhDGAT2a, we constructed pAhDGAT2a:GUS cassettes and introduced it into the tobacco SR1 genome by Agrobacterium-mediated transformation. Expression pattern was monitored by histochemical staining. Results showed that GUS activity driven by the pAhDGAT2a was detected in almost all vegetative and reproductive tissues, with a higher expression level in stigma, anther and young seeds than in the other organs, indicating thatpAhDGAT2a has a constitutive promoter activity.

Key words: Arachis hypogaea L., DGAT2a gene, Promoter, Function analysis

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