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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (03): 415-423.doi: 10.3724/SP.J.1006.2011.00415

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Correlation between AhFAD2 Polymorphism and Oleic Acid/ Linoleic Acid (O/L) Ratio in Peanut Seeds

ZHOU Li-Xia1,3,TANG Gui-Ying1,2,CHEN Gao1,3,BI Yu-Ping1,2,3,SHAN Lei1,2,*   

  1. 1 Hi-Tech Research Centre, Shandong Academy of Agricultural Sciences and Key Laboratory for Genetic Improvement of Crop Animal and Poultry of Shandong Province, Jinan 250100, China; 2 Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100, China; 3 College of Life Science, Shandong Normal University, Jinan 250014, China
  • Received:2010-08-02 Revised:2010-12-03 Online:2011-03-12 Published:2011-01-17

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Abstract: Peanut oils containing high levels of oleic acid and low linoleic acid levels are strongly demanded. This would improve oil stability and nutritional quality. Thus, breeding peanut variety with high O/L ratio has become one of the major goals in plant breeding. To investigate the genetic traits that control high oleate levels-phenotype and improve the peanut-breeding efficiency, we sequenced ORF region of about 527 delta 12 fatty acid desaturaseAhFAD2 cDNAs from 13 peanut varieties. Our results showed that three types of mRNA transcripts AhFAD2A, AhFAD2B and pseudogene existed in all peanut varieties. Genotypes of peanut varieties TaishanPearl, Taishan Sanlirou, Jiangtian, Chico, 05-21063, Baisha 1016; and Linguimake, Feilongxiang, Goule, Luhua 14, Huayu 19, Huayu 23, and Luhua 11 were found to be OL1OL1OL2OL2, ol1ol1OL2OL2 and OL1ol1OL2OL2, respectively. We also found thatsome SNP polymorphism sites existed in the AhFAD2A gene but not in all peanut varieties, and AhFAD2B was relatively conserved in all 13 peanut varieties investigated. Combined with the measurement of O/L ratio in various peanut varieties, the relationship between gene polymorphism and O/L ratio was primarily investigated. Meanwhile, the potential function of pseudogene was also discussed.

Key words: Arachis hypogaea L., Δ12 fatty acid desaturase gene, Gene polymorphism, O/L ratio, Pseudogene

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