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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (04): 754-759.doi: 10.3724/SP.J.1006.2012.00754

• RESEARCH NOTES • Previous Articles    

Karyotype Analysis of Arachis hypogaea L. Using Fluorescence Banding and Fluorescence in situ Hybridization with rDNA Probes

SHE Chao-Wen1,2,3,ZHANG Li-Hua1,JIANG Xiang-Hui1,2,3   

  1. 1 Department of Life Sciences, Huaihua University, Huaihua 418008, China; 2 Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua 418008, China; 3 Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education, Huaihua 418008, China
  • Received:2011-08-04 Revised:2011-12-19 Online:2012-04-12 Published:2012-02-13

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Abstract: The establishment of an exact and detailed karyotype of Arachis hypogaea L. was fundamental for clarification of the origin and research of the genome of the species. In this study, the mitotic metaphase chromosomes of the species were analyzed using DAPI banding and double fluorescence in situ hybridization (FISH) with 5S and 45S rDNA probes. The mean haploid karyotype length was (81.06±3.74) μm, the longest chromosome pair was (4.72±0.15) μm and the shortest chromosome pair was (2.62±0.14)μm. In the complements of the species, fifteen pairs of the chromosomes displayed centromeric DAPI+ bands including ten pairs of strong bands and five pairs of weak bands; and two pairs of 5S and five pairs of 45S rDNA sites were showed, with one 5S site being syntenic to a 45S site. Combining the chromosome measurements with DAPI+ bands and rDNA FISH signals, the chromosomes were exactly paired and arranged, and a detailed molecular cytogenetic karyotype of A. hypogaea is established. The karyotype formula of A. hypogaea was 2n=4x=40=38m+2sm (SAT) and the asymmetric karyotype belonged to 2A type.

Key words: Arachis hypogaea, Karyotype, Fluorochrome banding, rDNA, Fluorescence in situ hybridization

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