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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (03): 389-397.doi: 10.3724/SP.J.1006.2013.00389

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of Auxin Response Factor Gene (CsARF1) in Tea Plant (Camellia sinensis [L.] O. Kuntze)

HAO Xin-Yuan1,2,CAO Hong-Li2,YANG Ya-Jun2,*,WANG Xin-Chao2,*,MA Chun-Lei2,XIAO Bin1   

  1. 1 College of Horticulture, Northwest A&F University, Yangling 712100, China; 2 Tea Research Institute of Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China
  • Received:2012-07-20 Revised:2012-11-05 Online:2013-03-12 Published:2013-01-04
  • Contact: 杨亚军, E-mail: yjyang@mail.tricaas.com; 王新超, E-mail: xcw75@mail.tricaas.com, Tel: 0571-86653162

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Abstract:

Auxin response factors (ARFs) are transcription factors that bind to TGTCTC auxin response elements in promoters of early/primary response genes and regulate the expression of auxinresponse genes. The full-length cDNA of one ARF gene named CsARF1 (GenBank accession number JX307853) was firstly cloned from tea plant (Camellia sinensis [L.] O. Kuntze) by SMART-RACE-PCR. The results indicated that the full-length cDNA of CsARF1 was 3222 bp containing 2463 bp ORF which encoded 820 amino acid residues with a putative molecular mass of 49.35 kD. The soluble protein encoded by CsARF1 functioned in the cytoplasm and consisted of an amino-terminal DNA-binding domain (B3), a carboxy-terminal dimerization domain (IAA_ARF), and a Gln, Ser and Leu-rich middle region, which is proposed to function as an activation domain. Blast and phylogenetic analysis showed that the protein encoded by CsARF1 shared the highest identity (83%) with ARF8 in Vitisvinifera, and had close genetic relationship with ARF8 in Solanumlycopersicum. The expression analysis of CsARF1 conducted by qRT-PCT during the different phases of bud dormancy and bud break indicated that CsARF1 had a marked rise in the expression level at deep dormant stage and sprouting stage, demonstrating that CsARF1 is relevant to the regulation of tea plant bud dormancy and bud break.

Key words: Tea plant (Camellia sinensis), Dormancy, Auxin response factor, Expression analysis

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