cpt1基因与水稻根负向光性的关系

doi:10.3724/SP.J.1006.2009.01558

Abstract

Abstract:

With the purpose of studying the relationship between cpt1 gene and negative phototropism in rice roots, discussing the contribution of asymmetric distribution of IAA on negative phototropism in rice roots, CaCl₂, EDTA and IAA were assayed for their effects on the negative phototropism in rice roots, as well as effects on the expression of cpt1 gene on the basis of reverse transcription-PCR. The result showed that, the negative phototropism in rice roots was improved by the treatment with 1 mg L^-1 CaCl₂and 0.001 mg L^-1IAA in culture solution under light for 24 h but constrained by 1 mg L^-1EDTA. A similar effect was shown from the analysis of cpt1 gene expression, which suggested CPT1 protein could be induced by CaCl₂ and IAA. The effects also showed a positive correlation between the expression of cpt1 and negative phototropism in rice roots. It could be supposed from present results that the asymmetric distribution of IAA is an important step for the negative phototropism process in rice roots, which probably is particularly associated with CPT1 protein as a carrier of IAA.

Key words: Rice, Root, Negative phototropism, IAA carrier protein, cpt1 gene

WANG Yue-Xia,WANG Zhong,LIU Quan-Jun,ZHAO Hui-Jie,GU Yun-Jie,et al.. Relationship between cpt1 Gene and the Negative Phototropism in Rice Roots[J].Acta Agron Sin, 2009, 35(8): 1558-1561.

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Relationship between cpt1 Gene and the Negative Phototropism in Rice Roots

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