cpt1基因与水稻根负向光性的关系

doi:10.3724/SP.J.1006.2009.01558

Abstract

Abstract:

With the purpose of studying the relationship between cpt1 gene and negative phototropism in rice roots, discussing the contribution of asymmetric distribution of IAA on negative phototropism in rice roots, CaCl₂, EDTA and IAA were assayed for their effects on the negative phototropism in rice roots, as well as effects on the expression of cpt1 gene on the basis of reverse transcription-PCR. The result showed that, the negative phototropism in rice roots was improved by the treatment with 1 mg L^-1 CaCl₂and 0.001 mg L^-1IAA in culture solution under light for 24 h but constrained by 1 mg L^-1EDTA. A similar effect was shown from the analysis of cpt1 gene expression, which suggested CPT1 protein could be induced by CaCl₂ and IAA. The effects also showed a positive correlation between the expression of cpt1 and negative phototropism in rice roots. It could be supposed from present results that the asymmetric distribution of IAA is an important step for the negative phototropism process in rice roots, which probably is particularly associated with CPT1 protein as a carrier of IAA.

Key words: Rice, Root, Negative phototropism, IAA carrier protein, cpt1 gene

WANG Yue-Xia,WANG Zhong,LIU Quan-Jun,ZHAO Hui-Jie,GU Yun-Jie,et al.. Relationship between cpt1 Gene and the Negative Phototropism in Rice Roots[J].Acta Agron Sin, 2009, 35(8): 1558-1561.

References

[1] Iino M. Phototropism in higher plants. In: Hader D, Lebert M, eds. Photomovement: ESP Comprehensive Series in Photosciences, Vol. 1, Amsterdam: Elsevier, 2001. pp 659-811

[2] Tokutomi S, Matsuoka D, Zikihara K. Molecular structure and regulation of phototropin kinase by blue light. Biochim Biophys Acta, 2008, 1784: 133-142

[3] Khurana J P, Poff K L. Mutants of Arabidopsis thaliana with altered phototropism. Planta, 1989, 178: 400-406

[4] Liscum E, Briggs W R. Mutants of Arabidopsis in potential transduction and response components of the phototropic signaling pathway. Plant Physiol, 1996, 112: 291-296

[5] Kang B, Grancher N, Koyffmann V, Lardemer D, Burney S, Ahmad M. Multiple interactions between cryptochrome and phototropin blue-light signaling pathways in Arabidopsis thaliana. Planta, 2008, 227: 1091-1099

[6] Motchoulski A, Liscum E. Arabidopsis NPH3: A NPH1 photoreceptor-interacting protein essential for phototropism. Science, 1999, 286: 961-964

[7] Inoue S I, Kinoshita T, Matsumoto M, Nakayama K I, Doi M, Shimazaki K. Blue light-induced autophosphorylation of phototropin is a primary step for signaling. Proc Natl Acad Sci USA, 2008, 105: 5626-5631

[8] Pedmale U V, Liscum E. Regulation of phototropic signaling in Arabidopsis via phosphorylation state changes in the phototropin 1-interacting protein NPH3. J Biol Chem, 2007, 282: 19992-20001

[9] Sakai T, Kagawa T, Kasahara M, Swartz T E, Christie J M, Briggs W R, Wada M, Okada K. Arabidopsis nph1 and npl1: Blue light receptors that mediate both phototropism and chloroplast relocation. Proc Natl Acad Sci USA, 2001, 98: 6969-6974

[10] Fankhauser C, Yeh K C, Lagarias J C, Zhang H, Elich T D, Chory J. PKS1, a substrate phosphorylated by phytochrome that modulates light signaling in Arabidopsis. Science, 1999, 284: 1539-1541

[11] Lariguet P, Schepens I, Hodgson D, Pedmale U V, Trevisan M, Kami C, de Carbonnel M, Alonso J M, Ecker J R, Liscum E, Fankhauser C. Phytochrome kinase substrate 1 is a phototropin 1 binding protein required for phototropism. Proc Natl Acad Sci USA, 2006, 103: 10134-10139

[12] Sakai T, Wada T, Ishiguro S, Okada K. RPT2: A signal transducer of the phototropic response in Arabidopsis. Plant Cell, 2000, 12: 225-236

[13] Boccalandro H E, Simone S N D, Bergmann-Honsberger A, Schepens I, Fankhauser C, Casal J J. PHYTOCHROME KINASE SUBSTRATE1 regulates root phototropism and gravitropism. Plant Physiol, 2008, 146: 108-115

[14] Iino M. Mediation of tropisms by lateral translocation of endogenous indole-3-acetic acid in maize coleoptiles. Plant Cell Environ, 1991, 14: 279-286.

[15]Mo Y W, Wang Z, Qian S Q, Gu Y J. Effect of indoleacetic acid (IAA) on the negative phototropism of rice root. Rice Sci, 2004, 11(3): 125-128

[16] Haga K, Takano M, Neumann R, Iino M. The rice COLEOPTILE PHOTOTROPISM1 gene encoding an ortholog of Arabidopsis NPH3 is required for phototropism of coleoptiles and lateral translocation of auxin. Plant Cell, 2005, 17: 103-115

[17] Haga K, Iino M. Asymmetric distribution of auxin correlates with gravitropism and phototropism but not with autostraightening (autotropism) in pea epicotyls. J Exp Bot, 2006, 57: 837-847

[18] Harper R M, Stowe-Evans E L, Luesse D R, Muto H, Tatematsu K, Watahiki M K, Yamamoto K, Liscum E. The NPH4 locus encodes the auxin response factor ARF7, a conditional regulator of differential growth in aerial Arabidopsis tissue.Plant Cell, 2000, 12: 757-770

[19] Tatematsu K, Kumagai S, Muto H, Sato A, Watahiki M K, Harper R M, Liscum E, Yamamoto K T. MASSUGU2 encodes Aux/IAA19, an auxin-regulated protein that functions together with the transcriptional activator NPH4/ARF7 to regulate differential growth responses of hypocotyl and formation of lateral roots in Arabidopsis thaliana. Plant Cell, 2004, 16: 379-393

[20] Muday G K, Murphy A S. An emerging model of auxin transport regulation. Plant Cell, 2002, 14: 293-299

[21] Geldner N, Friml J, Stierhof Y D, Jurgens G, Palme K. Auxin transport inhibitors block PIN1 cycling and vesicle trafficking.Nature, 2001, 413: 425-428

[22] Friml J, Wisniewska J, Benkova E, Mendgen K, Palme K. Lateral relocation of Auxin efflux regulator PIN3 mediates tropism in Arabidopsis. Nature, 2002, 415: 806-809

[23]Gu Y-J(顾蕴洁), Wang Z(王忠), Wang W-X(王维学). The negative phototropism of rice root. Plant Physiol Commun (植物生理学通讯), 2001, 37(5): 396-398 (in Chinese with English abstract)

[24] Wang Z, Mo Y W, Qian S Q, Gu Y J. Negative phototropism of rice root and its influencing factors. Sci China (Ser C), 2002, 45(5): 485-496

[25] Harada A, Shimazaki K. Phototropins and blue light-dependent calcium signaling in higher plants. Photochem Photobiol, 2007, 83: 102-111

[26] Baum G, Long J C, Jenkins G I, Trewavas A J. Stimulation of the blue light phototropic receptor NPH1 causes a transient increase in cytosolic Ca2+. Proc Natl Acad Sci USA, 1999, 96: 13554-13559

[27] Iino M, Neumann R. Phototropism of rice seedlings: Characterization and mutant isolation. Plant Cell Physiol, 2000, 41(suppl): S56

Related Articles 15

[1]	TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2]	ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[3]	ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[4]	ZHENG Xiao-Long, ZHOU Jing-Qing, BAI Yang, SHAO Ya-Fang, ZHANG Lin-Ping, HU Pei-Song, WEI Xiang-Jin. Difference and molecular mechanism of soluble sugar metabolism and quality of different rice panicle in japonica rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1425-1436.
[5]	YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[6]	YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[7]	DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[8]	SUN Si-Min, HAN Bei, CHEN Lin, SUN Wei-Nan, ZHANG Xian-Long, YANG Xi-Yan. Root system architecture analysis and genome-wide association study of root system architecture related traits in cotton [J]. Acta Agronomica Sinica, 2022, 48(5): 1081-1090.
[9]	YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[10]	ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[11]	WANG Xiao-Lei, LI Wei-Xing, OU-YANG Lin-Juan, XU Jie, CHEN Xiao-Rong, BIAN Jian-Min, HU Li-Fang, PENG Xiao-Song, HE Xiao-Peng, FU Jun-Ru, ZHOU Da-Hu, HE Hao-Hua, SUN Xiao-Tang, ZHU Chang-Lan. QTL mapping for plant architecture in rice based on chromosome segment substitution lines [J]. Acta Agronomica Sinica, 2022, 48(5): 1141-1151.
[12]	PENG Xi-Hong, CHEN Ping, DU Qing, YANG Xue-Li, REN Jun-Bo, ZHENG Ben-Chuan, LUO Kai, XIE Chen, LEI Lu, YONG Tai-Wen, YANG Wen-Yu. Effects of reduced nitrogen application on soil aeration and root nodule growth of relay strip intercropping soybean [J]. Acta Agronomica Sinica, 2022, 48(5): 1199-1209.
[13]	WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[14]	KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[15]	CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Relationship between cpt1 Gene and the Negative Phototropism in Rice Roots

PDF

Cited