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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (1): 61-67.doi: 10.3724/SP.J.1006.2010.00061

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping QTLs for Uppermost Internode Diameter and Thickness and Area of Culm Wall with Doubled-Haploid Population in Wheat 

SANG Yun1,ZHAO Liang2,ZHANG Kun-Pu2,TIAN Ji-Chun2,*, YE Bao-Xing1,*   

  1. 1 College of Life Science / State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; 2 College of Agronomy / State Key Laboratory of Crop Biology / Group of Quality Wheat Breeding, Shandong Agricultural University, Tai’an 271018, China
  • Received:2009-04-16 Revised:2009-07-25 Online:2010-01-12 Published:2009-11-17
  • Contact: TIAN Ji-Chun, E-mail: jctian@sdau.edu.cn, Tel: 0538-8242040; 叶宝兴, E-mail: yeb@sdau.edu.cn, Tel: 0538-8242561

Abstract:

A population of 168 doubled haploid derived from two elite Chinese wheat (Triticum aestivum L.) cultivars Huapei 3 and Yumai 57, grown in two continuous cropping seasons in Tai’an, Shandong province, was used to investigate the genetic basis of uppermost internode diameter (UID) and thickness (CWT) and area (CWA) of culm wall in wheat. The quantitative trait loci (QTLs) for each trait were analyzed based on the constructed molecular linkage map of this population, including 324 SSR markers covering the whole wheat genome. The additive effects, epistatic effects, and their interactions with environment were identified by using the mixed linear model approach. A total of ten additive QTLs and six pairs of epistatic QTLs were detected. Among the ten QTLs, three had interactions with environment. For UID, four QTLs were detected on chromosomes 2D, 3D, and 5D (two regions), and explained 14.98% of phenotypic variation. Three QTLs for CWT were identified and accounted for 14.41% of the variation in the same or close region chromosome of UID, which showed pleiotropisms or tight linkages. Three QTLs on chromosome 3D, 4B and 5D controlling CWA explained 19.03% of the variation. Two intervals of XCFD53–XWMC18 and XWMC215–XBARC34 on chromosomes 2D and 5D for thickness and area of culm wall explained 11.37% and 10.98% phenotypic variation, respectively. They could be used in marker-assisted selection. For the six epistatic QTLs, the epistatic effects were not significant and without epistatic × environment interactions. These QTLs accounted for 19.01% of the observed phenotypic variation, without any additive effects (except qCWA-3D) of their own but involved in epistatic interactions. Such loci might play the role of modifying agents that tend to activate other loci or modify the action of other loci.

Key words: Wheat, Quantitative trait loci, Culm wll thickness, Uppermost internode, Doubled haploid population

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