小麦DH群体穗下节间直径、茎壁厚及茎壁面积的QTL定位

doi:10.3724/SP.J.1006.2010.00061

Abstract

Abstract:

A population of 168 doubled haploid derived from two elite Chinese wheat (Triticum aestivum L.) cultivars Huapei 3 and Yumai 57, grown in two continuous cropping seasons in Tai’an, Shandong province, was used to investigate the genetic basis of uppermost internode diameter (UID) and thickness (CWT) and area (CWA) of culm wall in wheat. The quantitative trait loci (QTLs) for each trait were analyzed based on the constructed molecular linkage map of this population, including 324 SSR markers covering the whole wheat genome. The additive effects, epistatic effects, and their interactions with environment were identified by using the mixed linear model approach. A total of ten additive QTLs and six pairs of epistatic QTLs were detected. Among the ten QTLs, three had interactions with environment. For UID, four QTLs were detected on chromosomes 2D, 3D, and 5D (two regions), and explained 14.98% of phenotypic variation. Three QTLs for CWT were identified and accounted for 14.41% of the variation in the same or close region chromosome of UID, which showed pleiotropisms or tight linkages. Three QTLs on chromosome 3D, 4B and 5D controlling CWA explained 19.03% of the variation. Two intervals of XCFD53–XWMC18 and XWMC215–XBARC34 on chromosomes 2D and 5D for thickness and area of culm wall explained 11.37% and 10.98% phenotypic variation, respectively. They could be used in marker-assisted selection. For the six epistatic QTLs, the epistatic effects were not significant and without epistatic × environment interactions. These QTLs accounted for 19.01% of the observed phenotypic variation, without any additive effects (except qCWA-3D) of their own but involved in epistatic interactions. Such loci might play the role of modifying agents that tend to activate other loci or modify the action of other loci.

Key words: Wheat, Quantitative trait loci, Culm wll thickness, Uppermost internode, Doubled haploid population

SANG Yun,ZHAO Liang,ZHANG Kun-Pu,TIAN Ji-Chun, YE Bao-Xing. Mapping QTLs for Uppermost Internode Diameter and Thickness and Area of Culm Wall with Doubled-Haploid Population in Wheat [J].Acta Agron Sin, 2010, 36(1): 61-67.

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Mapping QTLs for Uppermost Internode Diameter and Thickness and Area of Culm Wall with Doubled-Haploid Population in Wheat

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