Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (1): 61-67.doi: 10.3724/SP.J.1006.2010.00061


Mapping QTLs for Uppermost Internode Diameter and Thickness and Area of Culm Wall with Doubled-Haploid Population in Wheat 

SANG Yun1,ZHAO Liang2,ZHANG Kun-Pu2,TIAN Ji-Chun2,*, YE Bao-Xing1,*   

  1. 1 College of Life Science / State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; 2 College of Agronomy / State Key Laboratory of Crop Biology / Group of Quality Wheat Breeding, Shandong Agricultural University, Tai’an 271018, China
  • Received:2009-04-16 Revised:2009-07-25 Online:2010-01-12 Published:2009-11-17
  • Contact: TIAN Ji-Chun, E-mail: jctian@sdau.edu.cn, Tel: 0538-8242040; 叶宝兴, E-mail: yeb@sdau.edu.cn, Tel: 0538-8242561


A population of 168 doubled haploid derived from two elite Chinese wheat (Triticum aestivum L.) cultivars Huapei 3 and Yumai 57, grown in two continuous cropping seasons in Tai’an, Shandong province, was used to investigate the genetic basis of uppermost internode diameter (UID) and thickness (CWT) and area (CWA) of culm wall in wheat. The quantitative trait loci (QTLs) for each trait were analyzed based on the constructed molecular linkage map of this population, including 324 SSR markers covering the whole wheat genome. The additive effects, epistatic effects, and their interactions with environment were identified by using the mixed linear model approach. A total of ten additive QTLs and six pairs of epistatic QTLs were detected. Among the ten QTLs, three had interactions with environment. For UID, four QTLs were detected on chromosomes 2D, 3D, and 5D (two regions), and explained 14.98% of phenotypic variation. Three QTLs for CWT were identified and accounted for 14.41% of the variation in the same or close region chromosome of UID, which showed pleiotropisms or tight linkages. Three QTLs on chromosome 3D, 4B and 5D controlling CWA explained 19.03% of the variation. Two intervals of XCFD53–XWMC18 and XWMC215–XBARC34 on chromosomes 2D and 5D for thickness and area of culm wall explained 11.37% and 10.98% phenotypic variation, respectively. They could be used in marker-assisted selection. For the six epistatic QTLs, the epistatic effects were not significant and without epistatic × environment interactions. These QTLs accounted for 19.01% of the observed phenotypic variation, without any additive effects (except qCWA-3D) of their own but involved in epistatic interactions. Such loci might play the role of modifying agents that tend to activate other loci or modify the action of other loci.

Key words: Wheat, Quantitative trait loci, Culm wll thickness, Uppermost internode, Doubled haploid population

[1] Sasahara H, Fukuta Y, Fukuyama T. Mapping of QTLs for vascular bundle system and spike morphology in rice (Oryza sativa L.). Breed Sci, 1999, 49: 75-81

[2] Pan X-Y(潘学燕), Miao F(苗芳). Survey of wheat microtubule tissue structure. Chin Agric Sci Bull (中国农学通报), 2005, 21(9): 121-123 (in Chinese with English abstract)

[3] Kelbert A J, Spaner D, Briggs K G, King J R. The association of culm anatomy with lodging susceptibility in modern spring wheat genotypes. Euphytica, 2004, 136: 211-221

[4] Zhu L(朱蕾), Shi G-X(施国新), Li Z-S(李振声), Kuang T-Y(匡廷云), Li B(李冰), Wei Q-K(魏其克), Bai K-Z(白克智), Hu Y-X(胡玉熹), Lin J-X(林金星). Anatomical and chemical features of high-yield wheat cultivar with reference to its parents. Acta Bot Sin (植物学报), 2004, 46(5): 565-572 (in Chinese with English abstract)

[5]Börner A, Schumann E, Furste A, Coster H, Leithold B, Röder M S, Weber W E. Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet, 2002, 105: 921-936

[6]Li S S, Jia J Z, Wei X Y, Zhang X C, Li L Z, Chen H M, Fan Y D, Sun H Y, Zhao X H, Lei T D, Xu Y F, Jiang F S, Wang H G, Li L H. A intervarietal genetic map and QTL analysis for yield traits in wheat. Mol Breed, 2007, 20: 167-178

[7]Cui K H, Peng S B, Xing Y Z, Yu S B, Xu C G, Zhang Q. Molecular dissection of the genetic relationships of source, sink and transport tissue with yield traits in rice. Theor Appl Genet, 2003, 106: 649-658

[8] Qiu Z-F(裘昭峰), Fang C(方陈), Chen H-J(陈洪俭). On the vascular tissue of wheat internodal and its relationship to the grain number per spike. Acta Agron Sin (作物学报).1987, 13(2): 102-102 (in Chinese)

[9]Yuan T(远彤), Guo T-C(郭天财), Luo Y(罗毅), Sun J-H(孙金花). The relationship between leaf structure and grain formation of different kernel types in winter wheat. Acta Agron Sin (作物学报), 1998, 24(6): 876-883 (in Chinese with English abstract)

[10] Shen H-B(申海兵), Yang D-L(杨德龙), Jing R-L(景蕊莲), Chang X-P(昌小平), Qu Y-Y(曲延英). Genetic characters of vascular bundle in the first internode and its relationship with yield components of wheat. J Triticeae Crops (麦类作物学报), 2007, 27(3): 465-470 (in Chinese with English abstract)

[11] Li J-Z(李俊周), Liu Y-Y(刘艳阳), He N(何宁), Cui D-Q(崔党群). Genetic analysis of several quantitative traits of doubled haploid population in wheat. J Triticeae Crops (麦类作物学报), 2005, 25(3): 16-19 (in Chinese with English abstract)

[12] Zhang K-P(张坤普), Zhao L(赵亮), Hai Y(海燕), Chen G-F(陈广凤), Tian J-C(田纪春). QTL mapping for adult-plant resistance to powdery mildew, lodging resistance and internode length below spike in wheat. Acta Agron Sin (作物学报), 2008, 34(8): 1350-1357 (in Chinese with English abstract)

[13]Zhang K P, Zhao L, Tian J C, Chen G F, Jiang X L, Liu B. A genetic map conducted using a doubled haploid population derived from two elite Chinese common wheat (Triticum aestivum L.) varieties. J Integr Plant Biol, 2008, 50: 941-950

[14]Wang D L, Zhu J, Li Z K, Paterson A H. Mapping QTLs with epistatic effects and QTL × environment interactions by mixed linear model approaches. Theor Appl Genet, 1999, 99: 1255-1264

[15] Yang J, Zhu J. Predicting superior genotypes in multiple environments based on QTL effects. Theor Appl Genet, 2005, 110: 1268-1274

[16] Cao G, Zhu J, He C, Gao Y, Yan J, Wu P. Impact of epistasis and QTL × environment interaction on the developmental behavior of plant height in rice (Oryza sativa L.). Theor Appl Genet, 2001, 103: 153-160

[17] Xu M-Z(许明子), Quan X-L(全雪丽), Shi T-Y(石铁源), Zheng C-S(郑成淑), Liu X-H(刘宪虎). Study on conducting bundle character of neck and correlation of several rice breeds. J Agric Sci Yanbian Univ (延边大学农学学报), 2000, 22(2): 81-85 (in Chinese with English abstract)

[18]Ma J(马均), Zhou K-D(周开达), Ma W-B(马文波), Wang X-D(汪旭东), Tian Y-H(田彦华), Ming D-F(明东风), Xu F-Y(许凤英). The characteristics of the tissues of the first internode and their relations to the grain-filling for the different panicle types of rice. Acta Agron Sin (作物学报), 2002, 28(2): 215-220 (in Chinese with English abstract)

[19]Liu G L, Mei H W, Yu X Q, Zou G H, Liu H Y, Hu S P, Li M S, Wu J H, Chen L, Luo L J. QTL analysis of panicle neck diameter, a trait highly correlated with panicle size, under well-watered and drought conditions in rice (Oryza sativa L.). Plant Sci, 2008, 174: 71-77

[20]Zhang K-P(张坤普), Xu X-B(徐宪斌), Tian J-C(田纪春). QTL mapping for grain yield and spike related traits in common wheat. Acta Agron Sin (作物学报) 2009, 35(2): 270-278 (in Chinese with English abstract)

[21]Verma V, Foulkes M J, Worland A J, Sylvester-Bradley R, Caligari P D S. Mapping quantitative trait loci for flag leaf senescence as a yield determinant in winter wheat under optimal and drought-stressed environment. Euphytica, 2004, 135: 255-263

[22]Yang D L, Jing R L, Chang X P. Identification of quantitative trait loci and environmental interactions for accumulation and remobilization of water-soluble carbohydrates in wheat (Triticum aestivum L.) stems. Genetics, 2007, 176: 571-584

[23] Chen H-H(陈华华), Li J(李俊), Wan H-S(万洪深), Wang L-L(王玲玲), Peng Z-S(彭正松), Yany W-Y(杨武云). Microsatellite markers for culm wall thickness and anatomical features of solid stem wheat. Acta Agron Sin (作物学报), 2008, 34(8): 1381-1385 (in Chinese with English abstract)

[24]Atchley W R, Zhu J. Developmental quantitative genetics, conditional epigenetic variability and growth in mice. Genetics, 1997, 147: 765-776
[25] Xing Y-Z(邢永忠), Xu C-G(徐才国),Hua J-P(华金平), Tan Y-F(谈移芳). Analysis of QTL × environment interaction for rice panicle characteristics. Acta Genetic Sin (遗传学报), 2001, 28(5): 439-446 (in Chinese with English abstract)

[26] Anderson J A, Stack R W, Liu S. DNA markers for Fusarium head blight resistance QTL in two wheat populations. Theor Appl Genet, 2001, 102: 1164-1168

[27]Gao Y-M(高用明), Wan P(万平). Studies of marker screening efficiency and corresponding influencing factors in QTL composite interval mapping. Acta Genetic Sin (遗传学报), 2002, 29(6): 555-561 (in Chinese with English abstract)

[28]Wu W-R(吴为人), Tang D-Z(唐定中), Li W-M(李维明). Genetic dissection and molecular dissection of quantita tive traits. Acta Agron Sin (作物学报), 2000, 26(4): 501-507 (in Chinese with English abstract)
[29] Cao G Q, Zhu J, He C X, Gao Y M, Wu P. QTL analysis for epistatic and QTL × environment interactions on final height of rice (Oryza sativa L.). Acta Genetic Sin, 2001, 28: 135-143
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[3] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[4] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[5] FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[6] LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[7] XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
[8] ZHANG Yan-Bo, WANG Yuan, FENG Gan-Yu, DUAN Hui-Rong, LIU Hai-Ying. QTLs analysis of oil and three main fatty acid contents in cottonseeds [J]. Acta Agronomica Sinica, 2022, 48(2): 380-395.
[9] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[10] WANG Yang-Yang, HE Li, REN De-Chao, DUAN Jian-Zhao, HU Xin, LIU Wan-Dai, GU Tian-Cai, WANG Yong-Hua, FENG Wei. Evaluations of winter wheat late frost damage under different water based on principal component-cluster analysis [J]. Acta Agronomica Sinica, 2022, 48(2): 448-462.
[11] CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[12] MA Bo-Wen, LI Qing, CAI Jian, ZHOU Qin, HUANG Mei, DAI Ting-Bo, WANG Xiao, JIANG Dong. Physiological mechanisms of pre-anthesis waterlogging priming on waterlogging stress tolerance under post-anthesis in wheat [J]. Acta Agronomica Sinica, 2022, 48(1): 151-164.
[13] MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
[14] WEI Yi-Hao, YU Mei-Qin, ZHANG Xiao-Jiao, WANG Lu-Lu, ZHANG Zhi-Yong, MA Xin-Ming, LI Hui-Qing, WANG Xiao-Chun. Alternative splicing analysis of wheat glutamine synthase genes [J]. Acta Agronomica Sinica, 2022, 48(1): 40-47.
[15] LI Ling-Hong, ZHANG Zhe, CHEN Yong-Ming, YOU Ming-Shan, NI Zhong-Fu, XING Jie-Wen. Transcriptome profiling of glossy1 mutant with glossy glume in common wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2022, 48(1): 48-62.
Full text



No Suggested Reading articles found!