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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (07): 1216-1220.doi: 10.3724/SP.J.1006.2010.01216

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FISH Analysis of Resynthesized Allotetraploid Arabidopsis

LI Fang,ZHANG Gai-Sheng   

  1. Key Laboratory of Crop Heterosis of Shaanxi Province, Wheat Breeding Engineering Research Center, Ministry of Education, Northwest A&F University, Yangling 712100, China
  • Received:2009-10-26 Revised:2010-04-21 Online:2010-07-12 Published:2010-05-20
  • Contact: ZHANG Gai-Sheng, E-mail: zhanggsh@public.xa.sn.cn

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Abstract:

Allopolyploidy breeding or introduction of preferential gene(s) via allopolyploidization is widely attempted in production of new crops. Thus at the onset of establishment of new allopolyploids, the functional gametes are reproduced conditioning that normal events such as synapsis and pairing and correct segregation of homologous chromosomes are guaranteed with the avoidance of possible interference of homoeologous chromosomes during meiosis. In the present study, we reported on meiotic synapsis and pairing of homologous chromosomes during the development of pollen mother cells in the newly resynthesized allotetraploid Arabidopsis aided with DAPI staining and FISH technique. The results showed that the correct number of nuclear chromosomes and balanced segregation were frequently observed with DAPI staining, and FISH analysis further provided the improved resolution of synapsis and pairing of homologous chromosomes, and the investigated nuclear chromosomes derived from different parental lines had no interference with each other, and the synapsis and pairing of homologous chromosomes were clearly identified. Therefore, the results indicated that the newly resynthesized allotetraploid Arabidopsis may achieve the normal meiosis and propagate the functionalized gametes for fertilization, confirming a potential vigor of intra/interspecific hybridization and a cytological basis for allopolyploid breeding.

Key words: Arabidopsis, Allotertraploid, Resynthesized, DAPI, FISH


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