Abstract:

To investigate the genetic diversity among hulless barley (Hordeum vulgare var. nudum Hook. f.）varieties in the major agricultural areas of Tibet and to promote the efficient utilization of those varieties, the genetic diversity among 29 hulless barley varieties and 3 landraces from those areas were analyzed using PCR with intron-splice junction primers and long random primers. The results indicated that 39 distinct PCR bands in total were amplified using 5 primers selected, and 26 of them were polymorphic. A [1, 0] data matrix was established based on the PCR patterns, and the genetic similarity coefficients among those cultivars were calculated using Dice’s index of NTSYSpc 2.1 software. Cluster analysis of the hulless barley varieties was then conducted based on the genetic similarity coefficients using UPMGA method of the same software. The result showed that there were some diversities among those hulless barley cultivars, but in general, their genetic differences were less and the genetic distances were relatively narrow; and the dendrogram obtained from cluster analysis could reflect the distribution and the geographic characteristics of those cultivars clearly.

Key words: Hulless barley (Hordeum vulgare var. nudum Hook. f.), Germplasm resource, Genetic diversity, Cluster analysis