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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (09): 1577-1584.doi: 10.3724/SP.J.1006.2011.01577

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Analysis of Six Important Traits in Tobacco (Nicotiana tabacum L.)

LI Hua-Li1,CHEN Mei-Xia1,ZHOU Dong-Xin2,CHEN Shun-Hui3,TAO Ai-Fen1,LI Yan-Kun1,MA Hong-Bo1,QI Jian-Min1,*,GUO Yu-Chun1,*   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops / Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Fujian Longyan Tobacco Science Institute, Longyan 364000, China; 3 Fujian Tobacco Science Institute, Fuzhou 350003, China
  • Received:2010-12-21 Revised:2011-04-27 Online:2011-09-12 Published:2011-06-28
  • Contact: 祁建民, E-mail: Qijm863@163.com; 郭玉春, E-mail: ycguo168@sina.com

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Abstract: The agronomic traits, chemical components and diseases are very important factors affecting the yield and leaf quality of tobacco. QTLs linked to the three aspects of traits can be used in marker-assisted selection for the yield and leaf quality improvement in tobacco. The objective of this study was to identify QTLs controlling important traits in tobacco, which could promote the basic research of molecular marker-assisted selection breeding. A population with 127 F2 and F2:3 individuals from the cross between Taiyan 7 and Baile 21 was used to establish a genetic linkage map of tobacco, 190 marker loci were mapped into 26 linkage groups which spanned a total map length of 3 483 cM. With a randomized complete block design, two location field tests with three replicates one year were conducted to charactererize six important traits, including nicotine, total chlorine, total kalium, leaf length, leaf angle, and powdery mildew. Utilizing data of field trials and MCIM method, QTLs for six important characters were mapped and their genetic interactions were analyzed. As the results, two QTLs for nicotine, two QTLs for total chlorine, one QTL for total kalium, four QTLs for leaf length, one QTL for leaf angle, and one QTL for powdery mildew were detected, of which six QTLs had additive effect, and the others were epistatic. The results indicated that epistatic QTLs, the additive QTLs also played important roles in the genetic basis of the six important traits with eleven gene loci in tobacco.

Key words: Tobacco (Nicotiana tobacum L.), Trait, QTL, Epistasis

[1]Xiao B-G(肖炳光), Zhu J(朱军), Lu X-P(卢秀萍), Li Y-P(李永平), Bai Y-F(白永富). Genetic analysis for chemical constituents in flue-cured tobacco (Nicotiana tabacum L.). Acta Agron Sin (作物学报), 2005, 31(12): 1557–1561 (in Chinese with English abstract)
[2]Yang J, Zhu J. Methods for predicting superior genotypes under multiple environments based on QTL effects. Theor Appl Genet, 2005, 110: 1268–1274
[3]Julio E, Denoyes-Rothan B, Verrier J L, Dorlhac de Borne F. Detection of QTLs linked to leaf and smoke properties in Nicotiana tabacum based on a study of 114 recombinant inbred lines. Mol Breed, 2006, 18: 69–91
[4]Xiao B-G(肖炳光), Xu Z-L(徐照丽), Chen X-J(陈学军), Shen A-R(申爱荣), Li Y-P(李永平), Zhu J(朱军). Genetic linkage map constructed by using a DH population for the flue-cured tobacco. Acta Tabacaria Sin (中国烟草学报), 2006, 12(4): 35–40 (in Chinese with English abstract)
[5]Xiao B-G(肖炳光), Lu X-P(卢秀萍), Jiao F-C(焦芳蝉), Li Y-P(李永平), Sun Y-H(孙玉合), Guo Z-K(郭兆奎). Preliminary QTL analysis of several chemical components in flue-cured tobacco (Nicotiana tabacum L.). Acta Agron Sin (作物学报), 2008, 34(10): 1762–1769 (in Chinese with English abstract)
[6]Cai C-C(蔡长春), Chai L-G(柴利广), Wang Y(王毅), Xu F-S(徐芳森), Zhang J-J(张俊杰), Lin G-P(林国平). Construction of genetic linkage map of burley tobacco (Nicotiana tabacum L.) and genetic dissection of partial traits. Acta Agron Sin (作物学报), 2009, 35(9): 1646–1654 (in Chinese with English abstract)
[7]Bai D, Reeleder R, Brandle J E. Identification of two RAPD markers tightly linked with the Nicotiana debneyi for resistance to black root rot of tobacco. Theor Appl Genet, 1995, 91: 1184–1189
[8]Julio E, Verrier J L, Dorlhac de Borne F. Development of SCAR markers linked to three disease resistances based on AFLP within Nicotiana tabacum L. Theor Appl Genet, 2006, 112: 335–346
[9]Yi Y H, Rufty R C, Wernsman E A. Identification of RAPD markers linked to the wildfire resistance gene of tobacco using bulked segregant analysis. Tob Sci, 1998, 42: 52–57
[10]Julio E, Denoyes-Rothan B, Verrier J L, Dorlhac de Borne F. Detection of QTLs linked to leaf and smoke properties in Nicotiana tabacum based on a study of 114 recombinant inbred lines. Mol Breed, 2006, 18: 69–91
[11]Liu X-X(刘晓侠), Wang L(王荔), Wen G-S(文国松), Yang Y-Q(杨艳琼). Screening and study of RAPD markers linked to tobacco black shank resistance gene Bs1(t). Acta Agron Sin (作物学报), 2004, 30(5): 516–518 (in Chinese with English abstract)
[12]Milla S R, Levin J S, Lewis R S, Rufty R C. RAPD and SCAR markers linked to an introgressed gene conditioning resistance to Peronospora tabacina D. B. Adam. in tobacco. Crop Sci, 2005, 45: 2346–2354
[13]Moon H, Nicholson J S. AFLP and SCAR markers linked to tomato spotted wilt virus resistance in tobacco. Crop Sci, 2007, 47: 1887–1894
[14]Yang J, Hu C C, Ye X Z. QTL Network 2.0. Institute of Bioinformatics, Zhejiang University, Hangzhou, China, 2005
[15]McCouch S R, Cho Y G, Yang M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11–13
[16]Ma H-B(马红勃), Qi J-M(祁建民), Li Y-K(李延坤), Liang J-X(梁景霞), Wang T(王涛), Lan T(兰涛), Chen S-H(陈顺辉), Tao A-F(陶爱芬), Lin L-H(林荔辉), Wu J-M(吴建梅). Construction of a molecular genetic map of tobacco based on SRAP and ISSR markers. Acta Agron Sin (作物学报), 2008, 34(11): 1958–1963 (in Chinese with English abstract)
[17]Li Y-K(李延坤). Construction of Genetic Linkage Map of Tobacco (Nicotiana tabacum L.). MS Dissertation of Fujian Agriculture and Forestry University, 2009 (in Chinese with English abstract)
[18]Knapp S J, Bridges W C, Birkes D. Mapping quantitative trait loci using molecular marker linkage maps. Theor Appl Genet, 1990, 79: 583–592
[19]Cai H W, Morishima H. Genomie regions affecting seed shattering and seed dormancy in rice. Theor Appl Genet, 2000, 100: 840–846
[20]Richter T E, Pryor T J, Bennetzen J L, Hulbert S H. New rust resistance specificities associated with recombination in the Rpl complex in maize. Genetics, 1995, 141: 373–381
[21]Hammond K E, Jones J D G. Plant disease resistance genes: Unraveling how they work. Can J Bot  1996, 73(supp1-1): 495–505
[22]Witsenboer H, Kesseli R V, Fortin M, Stangellini M, Michelmore R W. Sources and genetic structure of a cluster of genes for resistance to three pathogens in lettuce. Thero Appl Genet, 1995, 91: 178–188
[23]Li Z K, Yu S B, Lafitte H R, Huang N, Courtois B, Hittalmani S, Vijayakumar C H M, Liu G F, Wang G C, Shashidhar H E, Zhuang J Y, Zheng K L, Singh V P, Sidhu J S, Srivantaneeyakul S, Khush G S. QTL × environment interactions in rice: I. Heading date and plant height. Theor Appl Genet, 2003, 108: 141–153
[24]Yadav R S, Bidinger F R, Hash C T, Yadav Y P, Yadav O P, Bhatnagar S K, Howarth C J. Mapping and characterisation of QTL × E interactions for traits determining grain and stover yield in pearl millet. Theor Appl Genet, 2003, 106: 512–520
[25]Zhang K P, Tian J C, Zhao L, Wang S S. Mapping QTLs with epistatic effects and QTL×environment interactions for plant height using a doubled haploid population in cultivated wheat. Genet Genomics, 2008, 35: 119–127
[26]Cao G-Q(曹刚强), Zhu J(朱军), He C-X(何慈信), Gao Y-M(高用明), Wu P(吴平). QTL analysis for epistatic effects and QTL × environment interaction effects on final height of rice (Oryza sativa L.). Acta Genet Sin (遗传学报), 2001, 28(2): 135–143(in Chinese with English abstract)
[27]Yu S B, Li J X, Xu C G, Tan Y F, Li X H, Zhang Q. Identification of quantitative trait loci and epistatic interactions for plant height and heading date in rice. Theor Appl Genet, 2002, 104: 619–625
[28]Shan D-P(单大鹏), Zhu R-S(朱荣胜), Chen L-J(陈立君), Qi Z-M(齐照明), Liu C-Y(刘春艳), Hu G-H(胡国华), Chen Q-S(陈庆生). Epistatic effects and QE interaction effects of QTLs for protein content in soybean. Acta Agron Sin (作物学报), 2009, 35(1): 41–47 (in Chinese with English abstract)
[29]Zhang X-L(张先亮), Gao J-S(高俊山), Song G-L(宋国立), Liu F(刘芳). Additive and epistatic effects QTL analysis on upland cotton CRI-G6. Mol Plant Breed (分子植物育种), 2009, 7(2): 312–320 (in Chinese with English abstract)
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