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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (03): 462-470.doi: 10.3724/SP.J.1006.2012.00462

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Protein Expression at Different Microspore Development Stages in Wheat Cultivar Shaannong 138

BI Hui-Hui1,**, YANG Hua-Rui1,**,MA Jun-Hui1,LIU Lu-Xiang2,WANG Cheng-She1,*,XU Xi-Tang1,ZOU Shu-Fang1,XIE Yan-Zhou1   

  1. 1 College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2011-06-30 Revised:2011-10-12 Online:2012-03-12 Published:2011-12-01

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Abstract: To understand the metabolic mechanism of wheat anther development, we analyzed the holoprotein of Shaannong 138 at mid-late mononuclear pollen and binuclear pollen stages using two-dimensional electrophoresis technique. More than 450 clear protein spots were identified, including 26 differentially expressed protein spots. We selected 14 protein spots with expression difference at least two times for further separation by means of matrix-assisted laser desorption time of flight mass spectrometry peptide mass fingerprint analysis (MALDI-TOF-MS) in isoelectric point (pI) of 4–7. After indexing database NCBInr using software Mascot, we identified 11 significant protein spots. These protein spots were classified into several groups based on function, i.e., UDP-glucose pyrophosphorylase (one spot), chlorophyll antibody-binding protein (one spot), pentatricopeptide repeat protein PPR566-6 (one spot), cysteine protease inhibitor (one spot), ubiquitin (one spot), S-adenosyl-L-cysteine hydrolase (two spots), oxygen-enhanced proteins (two spots), and putative proteins (two spots). These proteins are involved in glycometabolism, protein degradation, cell defense, and some other metabolic processes.

Key words: Protein expression, Microspore development, Two-dimensional electrophoresis, Anther culture, Anther cultural ability

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