Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (03): 454-461.doi: 10.3724/SP.J.1006.2012.00454

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development of Near-isogenic Lines with Different Wheat Wx Genes and Their Effects on Amylose Content and Noodle Quality

YU Chun-Hua 1,2,**,BIE Tong-De2,**,WANG Cheng1,ZHANG Xiao2,WU Rong-Lin2,CHENG Xiao-Ming2,WANG Can-Guo2,ZHAO Yun2,CHENG Shun-He 2,*   

  1. 1 Beijing Research Center for Information Technology in Agriculture / National Engineering Research Center for Information Technology in Agriculture, Beijing, 100097, China; 2 Yangzhou Academy of Agricultural Sciences / Yangzhou Branch of National Wheat Improvement Center, Yangzhou 225007, China
  • Received:2011-08-02 Revised:2011-12-15 Online:2012-03-12 Published:2012-01-04
  • Contact: 程顺和, E-mail: yzcsh1939@126.com

PDF

1482

Cited

3

Abstract: Granule bound starch synthase (GBSS), controlled by Wx gene, is a key enzyme for amylose synthesis. Wheat has three homologous Wx genes located on 4AL, 7BS, and 7DS. The purpose of this study was to understand the effects of wheat Wx genes on amylose content and noodle sensory quality. A waxy wheat line, Caiwx (aabbdd), was used as a donor parent for Wx null alleles and a high yield cultivar, Yangmai 01-2 (AABBDD), was used as a recurrent parent. A BC9F2 population was constructed through successive backcrossing assisted by pollen staining with I2-KI solution. Eight homozygous near isogenic lines (NILs) with different Wx gene combinations (AABBDD, AABBdd, AAbbDD, AAbbdd, aaBBDD, aaBBdd, aabbDD, aabbdd) were then identified by STS markers of Wx genes and GBSS isoenzyme markers in the BC9F4 generation.The amylose contents and noodle sensory quality of these NILs were evaluated. The amylose contents of the NILs ranged from 0.9% to 24.8% with significant difference among lines. There was a regularly descending trend of amylose content in genotypes as normal wheat (AABBDD) > single null allele genotypes (aaBBDD, AAbbDD, and AABBdd) > double null allele genotypes (aabbDD, aaBBdd, and AAbbdd) > waxy wheat (aabbdd). Genotype aaBBdd had the highest amylose content in the double-null-allele group, and genotype AAbbDD had the lowest amylose content in the single-null-allele group. This indicated that Wx-B1 allele plays the most important role in amylosesynthesis. It was also found that the amylose content reduction did not follow a directly linear relationship when the null allele number increased. Thus, we inferred that there exits gene interaction among different Wx alleles. The result of noodle sensory quality evaluation showed that there were significant differences among the eight NILs. All the noodle sensory scores of waxy wheat (aabbdd) on color, appearance, firmness, stickiness, as well as the total score, were the lowest among the eight NILs. The single null genotypes performed better noodle sensory quality than the other genotypes and the recurrent parent Yangmai 01-2. In the single-null-allele group, the aaBBDD genotype showed the best noodle sensory quality (scored 87.4). Noodles made of flour of aaBBDD genotype were as good as those made of Xuehua flour, a kind of high-quality flour for noodle-making. However, the other seven genotypes and Yangmai 01-2 had significantly lower noodle scores than Xuehua flour. Therefore, it is feasible to develop wheat cultivars with high noodle quality through genetic manipulation on Wx gene.

Key words: Wx gene, STS marker, Near isogenic lines, Amylose content, Noodle quality

[1]Clark J R, Robertson M, Ainsworth C C. Nucleotide sequence of a wheat (Triticum aestivum L.) cDNA clone encoding the waxy protein. Plant Mol Biol, 1991, 16: 1099–1100

[2]Nakamura T, Yamamori M, Hidaka S. Variation of starch granule proteins and chromosome mapping of their coding genes in common wheat. Theor Appl Genet, 1996, 93: 275–281

[3]Rahman S, Li Z, Batey I, Cochrane M P, Appels R, Morell M. Genetic alteration of starch functionality in wheat. J Cereal Sci, 2000, 31: 91–110

[4]Zhao X C, Sharp P J. An improved 1D-SDS-PAGE method for the identification of three breed wheat “waxy” proteins. J Cereal Sci, 1996, 23: 1191–1193

[5]Murai J, Taira T, Othta D. Isolation and characterization of three waxy genes encoding the granule-bound starch synthase in hexaploid wheat. Gene, 1999, 234: 71–79

[6]Vrinten P, Nakamura T. Molecular characterization of waxy mutations in wheat. Mol Gen Genet, 1999, 261: 463–471

[7]Shariflou M R, Hassani M M, Sharp P J. A PCR-based DNA marker for detection of mutant and normal alleles of the Wx-D1 gene of wheat. Plant Breed, 2001, 120: 121–124

[8]Briney A, Wilson R, Robert H P, Barclay I, Crosbie G, Appels R, Mike G K J. A PCR-based marker for selection of starch and potential noodle quality in wheat. Mol Breed, 1998, 4: 427–433

[9]Saito M, Vrinten P, Ishikawa G, Graybosch R, Nakamura T. A novel codominant marker for selection of the null Wx-B1 allele in wheat breeding programs. Mol Breed, 2009, 23: 209-217

[10]Song J-M(宋建民), Li B-Y(李保云), You M-S(尤明山), Liang R-Q(梁荣奇), Chang C(常成), Liu S-B(刘守斌), Tang Z-H(唐朝晖), Liu G-T(刘广田). Molecular identification of wheat granule bound starch synthase gene polymorphism. Acta Genet Sin (遗传学报), 2004, 31(1): 81–86 (in Chinese with English abstract)

[11]Liu Y-C(刘迎春), Zhu H-L(朱惠兰), Cheng S-H(程顺和), Ma Z-Q(马正强). PCR-based molecular markers for Wx-A1 and Wx-D1 genes of wheat. J Triticeae Crops(麦类作物学报), 2005, 25(1): 1–5 (in Chinese with English abstract)

[12]Nakamura T, Yamamori M, Hirano H, Hidaka S. Identification of three Wx protein in wheat (Triticum aestivum L.). Biochem Genet, 1993, 31: 75–86

[13]Wang Z-N(王子宁), Guo B-H(郭北海), Li H-J(李洪杰), Zhang Y-M(张艳敏), Wen Z-Y(温之雨). SDS-PAGE for the detection of Wx protein in polyploid wheats. Hereditas (遗传), 2000, 22(3): 169–171 (in Chinese with English abstract)

[14]Pan Z-F(潘志芬), Deng G-B(邓光兵), Wang T(王涛), Yu M-Q(余懋群). An improved 1D-SDS-PAGE method for identification of wheat waxy protein. Chin J Appl Environ Biol (应用环境生物学报), 2000, 6(5): 487–489 (in Chinese with English abstract)

[15]Wickramasinghe H A M, Miura H. Gene dosage effect of the wheat Wx alleles and their interaction on amylose synthesis in the endosperm. Euphytica, 2003, 132: 303–310

[16]Araki E, Miura H, Sawada S. Identification of genetic loci affecting amylose content and agronomic traits on chromosome 4A of wheat. Theor Appl Genet, 1999, 98: 977–984

[17]Zhai H-M(翟红梅), Tian J-C(田纪春). Development of wheat mutants carrying different null Wx alleles and their starch properties. Acta Agron Sin (作物学报), 2007, 33(7): 1059–1066 (in Chinese with English abstract)

[18]Zhang Y(张艳), Yan J(阎俊), Chen X-M(陈新民), He Z-H(何中虎). Effect of blending Waxy wheat flour with common wheat on protein and starch properties and Chinese fresh noodle quality. J Triticeae Crop (麦类作物学报), 2007, 27(5): 803–808 (in Chinese with English abstract)

[19]Qin P(覃鹏), Ma C-X(马传喜), Wu R-L(吴荣林), Cheng S-H(程顺和). Effect of waxy wheat flour blending on quality of dry white Chinese noodle. J Chin Cereal Oils Assoc (中国粮油学报), 2008, 23(3): 17–23 (in Chinese with English abstract)

[20]Song J-M(宋建民), Liu A-F(刘爱峰), You M-S(尤明山), Li B-Y(李保云), Wu X-Y(吴祥云), Zhao Z-D(赵振东), Liu G-T(刘广田). Effects of waxy flour blending on starch pasting properties and noodle quality of nonwaxy flour. Sci Agri Sin (中国农业科学) , 2004, 37(12): 1838–1842 (in Chinese with English abstract)

[21]Liu J-J(刘建军), He Z-H(何中虎), Yang J (杨金), Xu Z-H(徐兆华), Liu A-F(刘爱峰), Zhao Z-D(赵振东). Variation of starch properties in wheat cultivars and their relationship with dry white Chinese noodle quality. Sci Agric Sin (中国农业科学), 2003, 36(1): 7–12 (in Chinese with English abstract)

[22]Miura H, Wickramasinghe M H A, Subasinghe R M, Araki E, Komae K. Development of near-isogenic lines of wheat carrying different null Wx alleles and their starch properties. Euphytica, 2002, 123: 353–359

[23]Tian J-C(田纪春). Theory and Method for Cereal Quality Test (谷物品质测试理论与方法). Beijing: Science Press, 2006. pp 191–193 (in Chinese)

[24]Zhang Y(张艳), Yan J(阎俊), Yoshida H, Wang D-S(王德森), Chen D-S(陈东升), Nagamine T, Liu J-J(刘建军), He Z-H(何中虎). Standardization of laboratory processing of Chinese white salted noodle and its sensory evaluation system. J Triticeae Crop (麦类作物学报), 2007, 27(1): 158–165 (in Chinese with English abstract)

[25]Yu C-H(于春花), Ma D-G(马德高), Cheng S-H(程顺和), Bie T-D(别同德), Zhang X-X(张晓祥), Wu R-L(吴荣林). Study on breeding Wx wheat lines with pollen endosperm staining with I2-KI solution. J Triticeae Crop (麦类作物学报), 2010, 30(5): 835–841 (in Chinese with English abstract)

[26]Song J-M(宋建民), Liu A-F(刘爱峰), Cheng D-G(程敦公), Dai S(戴双), Li H-S(李豪圣), Liu J-J(刘建军), Zhao Z-D(赵振东). Effect of different Wx protein combinations on wheat starch biosynthesis in developing endosperm. Sci Agric Sin (中国农业科学), 2009, 42(3): 765–771 (in Chinese with English abstract)

[27]Wang L(王亮), Mu P-Y(穆培源), Xu H-J(徐红军), Zhuang L(庄丽), Sang W(桑伟), Nie Y-B(聂迎彬), Cui F-J(崔凤娟), Zou B(邹波). Characterization of Wx genes and starch pasting properties of Xinjiang winter and spring wheat cultivars. J Triticeae Crop (麦类作物学报), 2010, 36(6): 1017–1022 (in Chinese with English abstract)

[28]X-Z(王宪泽), Li H(李菡), Yu Z-W(于振文), Zhang J-D(张杰道). The path analysis of wheat grain in quality traits affecting noodle quality. Acta Agron Sin (作物学报), 2002, 28(2): 240–244 (in Chinese with English abstract)

[29]Baik B K, Lee M R. Effects of starch amylose content of wheat on textural properties of white salted noodles. Cereal Chem, 2003, 80: 304–309

[30]Lei Z-S(雷振生), Liu L(刘丽), Wang M-F(王美芳), Yan J(阎俊), Zhang Y(张艳), He Z-H(何中虎). Effect of HMW and LMW glutenin subunits on processing quality in common wheat. Acta Agron Sin (作物学报), 2009, 35(2): 203–210 (in Chinese with English abstract)

[31]Chen D-S(陈东升), Kiribuchi-Otobe C, Xu Z-H(徐兆华), Chen X-M(陈新民), Zhou Y(周阳), He Z-H(何中虎), Yoshida H, Zhang Y(张艳), Wang D-S(王德森). Effect of Wx-A1, Wx-B1 and Wx-D1 protein on starch properties and Chinese fresh noodle quality. Sci Agric Sin (中国农业科学), 2005, 38(5): 865–873 (in Chinese with English abstract)
[1] ZHANG Xiao, YAN Yan, WANG Wen-Hui, ZHENG Heng-Biao, YAO Xia, ZHU Yan, CHENG Tao. Application of continuous wavelet analysis to laboratory reflectance spectra for the prediction of grain amylose content in rice [J]. Acta Agronomica Sinica, 2021, 47(8): 1563-1580.
[2] ZHAO Chun-Fang,YUE Hong-Liang,TIAN Zheng,GU Ming-Chao,ZHAO Ling,ZHAO Qing-Yong,ZHU Zhen,CHEN Tao,ZHOU Li-Hui,YAO Shu,LIANG Wen-Hua,LU Kai,ZHANG Ya-Dong,WANG Cai-Lin. Physicochemical properties and sequence analysis of Wx and OsSSIIa genes in japonica rice cultivars from Jiangsu province and northeast of China [J]. Acta Agronomica Sinica, 2020, 46(6): 878-888.
[3] YANG Yong,LU Yan,GUO Shu-Qing,SHI Zhong-Hui,ZHAO Jie,FAN Xiao-Lei,LI Qian-Feng,LIU Qiao-Quan,ZHANG Chang-Quan. Improvement of rice eating quality and physicochemical properties by introgression of Wx in allele in indica varieties [J]. Acta Agronomica Sinica, 2019, 45(11): 1628-1637.
[4] YAO Shu, CHEN Tao,ZHANG Ya-Dong,ZHU Zhen,ZHAO Qing-Yong,ZHOU Li-Hui,ZHAO Ling,ZHAO Chun-Fang,WANG Cai-Lin*. Pyramiding Pi-ta, Pi-b and Wx-mq Genes by Marker-assisted Selection in Rice (Oryza sativa L.) [J]. Acta Agron Sin, 2017, 43(11): 1622-1631.
[5] MAO Ting,LI Xu,LI Zhen-Yu,XU Zheng-Jin. Development of PCR Functional Markers for Multiple Alleles of Wx and Their Application in Rice [J]. Acta Agron Sin, 2017, 43(11): 1715-1723.
[6] KONG Xin-Xin,ZHANG Yan,ZHAO De-Hui,XIA Xian-Chun,WANG Chun-Ping,HE Zhong-Hu. Noodle Quality Evaluation of New Wheat Cultivars from Northern China Winter Wheat Regions [J]. Acta Agron Sin, 2016, 42(08): 1143-1159.
[7] YAO Jian-Qiang,BAO Jian-Dong,ZHU Jin-Qing,GUI Yi-Jie,SHEN Qiu-Fang,HU Wei-Min,FAN Long-Jiang. Genetic Diversity of Waxy Gene in Chinese Glutinous Maize [J]. Acta Agron Sin, 2013, 39(01): 43-49.
[8] WU Hun-Lun, ZHAO Yuan, CHI De-Zhao. Relationship between Polymorphism at Wx Gene and Amylose Content in Hulless Barley [J]. Acta Agron Sin, 2012, 38(01): 71-79.
[9] GU Dan-Dan, LIU Zheng-Hui, LIU Yang, WANG Chao-Hua, WANG Jiang-Cheng, LI Gang-Hua, DING Yan-Feng. Effect of Lipid Content and Components on Cooking Quality and Their Response to Nitrogen in Milled Japonica Rice [J]. Acta Agron Sin, 2011, 37(11): 2001-2010.
[10] ZHANG Yan, TANG Jian-Wei, Geoffroy D’HUMIERES, HE Zhong-Hu. Correlation between Mixolab Parameter and Mixograph and RVA Parameters and Its Effect on Noodle Quality [J]. Acta Agron Sin, 2011, 37(08): 1441-1448.
[11] WANG Xin-Qi, YAN Li-Qing, LU You-Lin, CHEN Ge-Zhi. Quality Improvement of Long-te-fu Male Sterile Lines with Antisense Wx Gene and Associated Application in Rice Breeding [J]. Acta Agron Sin, 2010, 36(09): 1512-1518.
[12] YANG Yu-Shuang,PANG Bin-Shuang,WANG Lan-Fen,ZHANG Xue-Yong,YU Yuan-Jie. Complementary Effect of High Molecular Weight Glutenin Subumits on Bread-Making Quality in Common Wheat [J]. Acta Agron Sin, 2009, 35(8): 1379-1385.
[13] REN Yan;LIANG Dan;ZHANG Ping-Ping;HE Zhong-Hu;CHEN Jing;FU Ti-Hua;XIA Xian-Chun. Characterization of Overexpressed Bx7 Gene(Bx7OE) in Chinese and CIMMYT Wheats by STS Markers [J]. Acta Agron Sin, 2009, 35(3): 403-411.
[14] TANG Huai-Jun,YIN Gui-Hong,XIA Xian-Chun,FENG Jian-Jun,QU Yan-Ying,HE Zhong-Hu. Evaluation of Molecular Markers Specific for 1BL·1RS Translocation and Characterization of 1RS Chromosome in Wheat Varieties from Different Origins [J]. Acta Agron Sin, 2009, 35(11): 2107-2115.
[15] CAO Ya-Ping,CAO Ai-Zhong,WANG Xiu-E,CHEN Pei-Du. Screening and Application of EST-based PCR Markers Specific to Individual Chromosomes of Haynaldia villosa [J]. Acta Agron Sin, 2009, 35(1): 1-10.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd