Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (09): 1512-1518.doi: 10.3724/SP.J.1006.2010.01512

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Quality Improvement of Long-te-fu Male Sterile Lines with Antisense Wx Gene and Associated Application in Rice Breeding

WANG Xin-Qi, YIN Li-Qing, LU You-Lin, and SHEN Ge-Zhi*   

  1. Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
  • Received:2010-01-29 Revised:2010-04-18 Online:2010-09-12 Published:2010-06-11
  • Contact: SHEN Ge-Zhi,E-mail:Gzshen2003@yahoo.com.cn,Tel:021-62208184

PDF

1113

Abstract: In this study, we have generated the Long-te-fu new maintain lines B (wxLB1, wxLB2) with the low amylose content (AC) in seeds of these lines by trans-antisense Wx gene, and obtained the corresponding new male sterile lines of Xin-long-bao A (wxLA1, wxLA2) by transferring breeding. The agronomic traits, amylose content, gel consistency (GC) and the RVA profile eigenvalue of hybrid offspring by the new sterile lines and the original male sterile lines crossed with restorers respectively were tested, the results showed that the amylose content and grain weight were extremely significant lower than the respective control, the gel consistency (GC) and breakdown viscosity (BDV) were higher than the respective control obviously, and reached the extremely significant level. And the cold viscosity (CPV), setback (SBV), peak time (PeT), pasting temperature (PaT) and consistence (CSV) were significant or extremely significant lower than the respective control. The analysis of variation source on gel consistency and RVA eigenvalue of hybrid offspring indicated that starch GC and the RVA eigenvalue mainly affected by the female parent and both reached to the extremely significant level. The integrative results showed that the improvement quality of the male sterile lines of Long-te-fu was an effective way to meliorate the starch physico-chemical and cooking quality of hybrid in indica rice.

Key words: Wx gene, Low amylase content, Male sterile line, Quality improvement, Indica

[1] Huang R-F(黄蓉芬), Zhong H-M(钟海明), Xie X-B(谢小彬), Liu M-N(柳美南), Yan C-L(颜春龙), Chen B(陈波). Progress of research on inheritance of amylose content in rice grain. Acta Agric Jiangxi (江西农业学报), 2006, 18(6): 21-24 (in Chinese with English abstract)
[2] Zhu C-L(朱昌兰), Shen W-B(沈文飚), Zhai H-Q(翟虎渠), Wan J-M(万建民). Advances in researches of the application of low-amylose content rice gene for breeding. Sci Agric Sin (中国农业科学), 2004, 37(2): 157-162 (in Chinese with English abstract)
[3] Peng L-J(彭灵佳), Xia C-L(肖层林). Progress of research rice quality genetics and breeding in hybrid rice. Crop Res (作物研究), 2006, (5): 405-408 (in Chinese with English abstract)
[4] Liu H-Y(刘海英), Li X-G(李晓光), Liu H-L(刘宏亮), Huang X(黄星), Xu M-L(徐美兰), Jin Z-X(金正勋). Relation of amylose content selection with quality and yield traits of cross progenies in rice. Chin J Rice Sci (中国水稻科学), 2009, 23(1): 103-106 (in Chinese with English abstract)
[5] Wu D-X(吴殿星), Shu Q-Y(舒庆尧), Xia Y-W(夏英武). Rapid identification of starch viscosity property of early indica rice varieties with different apparent amylose content by RVA profile. Chin J Rice Sci (中国水稻科学), 2001, 15(1): 57-59 (in Chinese with English abstract)
[6] Zhu M-S(朱满山), Tang S-Z(汤述翥), Gu M-H(顾铭洪). Progresses in the study on the assessing，genetic and breeding of the rice starch RVA profile in rice eating quality. Chin Agric Sci Bull (中国农学通报), 2005, 21(8): 59-64 (in Chinese with English abstract)
[7] Shen G-Z(沈革志), Wang X-Q(王新其), Yin L-Q(殷丽青), Cai X-L(蔡秀玲), Wang Z-Y(王宗阳). Rapidly obtaining the markless transgenic rice with reduced amylose content by co-transformation and anther culture. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2004, 30(6): 637-643 (in Chinese with English abstract)
[8] Shen G-Z(沈革志), Wang X-Q(王新其), Yin L-Q(殷丽青), Wang H-M(王慧梅). Determination of amylose content in transgenic progenies containing an antisense waxy gene and identifying of pure lines in indica rice. Acta Biol Exp Sin (实验生物学报), 2004, 37(6): 429-436 (in Chinese with English abstract)
[9] Ding S(丁盛), Liu Q(刘清), Huang Z-G(黄志刚), Zhou J-J(周建军), Xiao L-T(萧浪涛). Genetic transformation of indica rice 9311 with antisense waxy gene
[J].J Hunan Agric Univ (Nat Sci)(湖南农业大学学报·自然科学版.2009, 35(4):366-368
[10] Wang X-Q(王新其), Shen G-Z(沈革志), Cheng L(程磊), Yin L-Q(殷丽青). The genetic stability of antisense waxy gene segment in the progeny of transgenic plants in rice. Acta Agric Shanghai (上海农业学报), 2003, 19(2): 12-16 (in Chinese with English abstract)
[11] Liu Q Q, Wang Z Y, Chen X H, Cai X L, Tang S Z, Yu H X, Zhang J L, Hong M M, Gu M H. Stable inheritance of the antisense waxy gene in transgenic rice with reduced amylose level and improved quality. Transgenic Res,2002, 11: 1-12
[12] Liu Q Q, Yu H X, Chen X H, Cai X L, Tang S Z, Wang Z Y, Gu M H. Field performance of transgenic indica hybrid rice with improved cooking and eating quality by down-regulation of Wx gene expression
[J].Mol Breed
[13] Zhou P H, Tan Y F, He Y Q, Xu C G, Zhang Q. Simultaneous improvement for four quality traits of Zhenshan 97, an elite parent of hybrid rice by molecular marker-assisted selection. Theor Appl Genet, 2003, 106: 326-331
[14] Wang Y(王岩), Fu X-M(付新民), Gao G-J(高冠军), He Y-Q(何予卿). Improving the grain quality of Minghui 63, a restorer line of rice with good quality through marker-assisted selection. Mol Plant Breed (植物分子育种), 2009, 7(4): 661-665 (in Chinese)
[15] Chen S(陈 圣), Ni D-H(倪大虎), Lu X-Z(陆徐忠), Song F-S(宋丰顺), Zhang S-L(张士陆), Wang Z-Y(王宗阳), Cai X-L(蔡秀玲), Li Z-F(李泽福), Wang X-F(汪秀锋), Li L(李 莉), Yang J-P(杨剑波). Reducing amylose content of hybrid rice Xieyou 57 by molecular marker-assisted selection. Chin J Rice Sci (中国水稻科学), 2008, 226: 597-602 (in Chinese with English abstract)
[16] Wang X-Q(王新其), Ying L-Q(殷丽青), Shen G-Z(沈革志), Xu L(徐 丽), Liu Q-Q(刘巧泉). Analysis of relationship between RVA profile and amylose content of the transgenic rice with antisense Wx gene. Sci Agric Sin (中国农业科学), 2009, 42(6): 1881-1888 (in Chinese with English abstract)
[17] Bao J S. Xia Y W. Genetic control of paste viscosity characteristics in indica rice (Oryza sativa L.). Theor Appl Genet, 1999, 98: 1120-1124
[18] Shui J-M(隋炯明), Li X(李 欣), Yan S(严松), Yan C-J(严长杰中国农业科学), 2005, 38(4): 657-663 (in Chinese with English abstract)), Zhang R(张蓉), Tang S-Z(汤述翥), Lu J-F(陆驹飞), Cheng Z-X(陈宗祥), Gu M-H(顾铭洪). Studies on the rice RVA profile characteristics and its correlation with the quality. Sci Agric Sin (
[19] Zhu M-S(朱满山), Gu M-H(顾铭洪), Tang S-Z(汤述翥). Correlation analysis of starch RVA profile and cooking physical-chemical Indices of different cultivars (lines) and DH populations japonica rice. Acta Agron Sin (作物学报), 2007, 33(3): 411-418 (in Chinese with English abstract)
[20] Jin J(金军), Xu D-Y(徐大勇), Cai Y-X(蔡一霞), Hu S-Y(胡署云), Ge M(葛敏), Zhu Q-S(朱庆森). Effect of N-fertilizer on main quality characters of rice and RVA profile parameters. Acta Agron Sin (作物学报), 2004, 30(2): 154-158 (in Chinese with English abstract)
[21] Li X(李欣), Zhang R(张蓉隋炯明), Liang G-H(梁国华), Shen X-P(沈新平), Yan C-J(严长杰), Gu S-L(顾世梁), Gu M-H(顾铭洪). Performance and inheritance of rice starch viscosity (RVA profile) characteristics. Chin J Rice Sci (中国水稻科学), 2004, 18(5): 384-390 (in Chinese with English abstract)), Shui J-M(
[22] Wang L Q, Liu W J, Xu Y, He Y Q, Luo L J, Xing Y Z, Xu C G, Zhang Q F. Genetic basis of 17 traits and viscosity parameters characterizing the eating and cooking quality of rice grain
[J].Theor Appl Genet
[23] Standard Issued by Ministry of Agriculture, P R China (中华人民共和国农业部部标准), NY147-88: Method for Rice Quality Measurement (米质测定方法). Beijing: Chinese Standard Press, 1988.pp 4-6 (in Chinese)
[24] Liu Q-Q(刘巧泉), Wang X-W(王兴稳), Chen X-H(陈秀花), Wang Z-Y(王宗阳), Tang S-Z(汤述翥), Hong M-M(洪孟民), Gu M-H(顾铭洪中国农业科学), 2002, 35(2): 117-122 (in Chinese with English abstract)). Effect of dominant waxy character on kernel weight of transgenic rice with antisense Wx gene. Sci Agric Sin (
[25] Wu H-K(吴洪恺), Liu S-J(刘世家), Zhang W-W(张文伟), Liu L-L(刘玲珑), Zhao Z-G(赵志刚). Relationship between lipid and flour viscosity characteristics in rice (Oryza sativa L.). Jiangsu Agric Sci (江苏农业学报), 2009, 25(3): 464-468 (in Chinese with English abstract)
[26] Wang F(王丰). Progress of research on inheritance of the quality in hybrid rice. Guangdong Agric Sci (广东农业科学), 2005, (5): 11-14 (in Chinese)
[1] JIANG Jian-Hua, ZHANG Wu-Han, DANG Xiao-Jing, RONG Hui, YE Qin, HU Chang-Min, ZHANG Ying, HE Qiang, WANG De-Zheng. Genetic analysis of stigma traits with genic male sterile line by mixture model of major gene plus polygene in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2021, 47(7): 1215-1227.
[2] FANG Ya-Jie,ZHU Ya-Jun,WU Zhi-Chao,CHEN Kai,SHEN Cong-Cong,SHI Ying-Yao,XU Jian-Long. Genome-wide Association Study of Grain Appearance and Milling Quality in a Worldwide Collection of Indica Rice Germplasm [J]. Acta Agron Sin, 2018, 44(01): 32-42.
[3] MAO Ting,LI Xu,LI Zhen-Yu,XU Zheng-Jin. Development of PCR Functional Markers for Multiple Alleles of Wx and Their Application in Rice [J]. Acta Agron Sin, 2017, 43(11): 1715-1723.
[4] SUN Hui**,ZHANG Feng-Ting**,WANG Yong-Bo,YE Zhi-Jie,QIN Zhi-Lie,BAI Xiu-Cheng,YANG Ji-Fang,GAO Jian-Gang,ZHAO Chang-Ping. Fertility Alteration in Male Sterile Line BS210 of Wheat [J]. Acta Agron Sin, 2017, 43(02): 171-178.
[5] JU Cheng-Xin,TAO Jin,QIAN Xi-Yang,GU Jun-Fei,ZHANG Hao,ZHAO Bu-Hong,LIU Li-Jun,WANG Zhi-Qin,YANG Jian-Chang. Leaf Photosynthetic Characteristics of Mid-season Indica Rice Varieties Applied at Different Decades [J]. Acta Agron Sin, 2016, 42(03): 415-426.
[6] XU Jun-Wei,MENG Tian-Yao,JING Pei-Pei,ZHANG Hong-Cheng,LI Chao,DAI Qi-Gen,WEI Hai-Yan,GUO Bao-Wei. Effect of Mechanical-Transplanting Density on Lodging Resistance and Yield in Different Types of Rice [J]. Acta Agron Sin, 2015, 41(11): 1767-1776.
[7] GONG Jin-Long,XING Zhi-Peng,HU Ya-Jie,ZHANG Hong-Cheng*,DAI Qi-Gen,HUO Zhong-Yang,XU Ke,WEI Hai-Yan,GAO Hui,GUO Bao-Wei. Difference of Root Morphological and Several Physiological Characteristics between Indica and Japonica Super Rice Varieties [J]. Acta Agron Sin, 2014, 40(06): 1066-1080.
[8] ZHANG Guo-Jia,HOU Lei,WANG Qing-Guo,LI Zhen,DAI Shao-Jun,LIU Wei. Cloning and Functional Characterization of Peanut Gene AhSOS2 [J]. Acta Agron Sin, 2014, 40(03): 405-415.
[9] ZHANG Hong-Cheng,XU Ke,ZHANG Jun,LI Guo-Ye,DONG Xiao-Bo,HUA Jin,ZHOU Pei-Jian,CHENG Fei-Hu,HUANG Da-Shan,CHEN Zhong-Ping,CHEN Guo-Liang,FANG Ming-Zhen,DAI Qi-Gen,HUO Zhong-Yang,WEI Hai-Yan,GAO Hui. Productivity and Eco-physiological Characteristics of Late Japonica Rice in Double-Cropping Rice System [J]. Acta Agron Sin, 2014, 40(02): 283-300.
[10] LEI Dong-Yang,TANG Wen-Bang,XIE Zhi-Jian,LIU Hai,CHEN Li-Yun*. How to Tackle Insecurity Problems in Seed Production of Two-line Hybrid Rice [J]. Acta Agron Sin, 2013, 39(09): 1569-1575.
[11] XU Quan,TANG Liang,XU Fan,FUKUSHIMA Akira,HUANG Rui-Dong,CHEN Wen-Fu,XU Zheng-Jin. Research Advances and Prospects of Eating Quality Improvement in Japonica Rice (Oryza sativa L.) [J]. Acta Agron Sin, 2013, 39(06): 961-968.
[12] ZHANG Rong-Ping,MA Jun,CAI Guang-Ze,SUN Yong-Jian. Effects of Low Temperature Stress during Flowering Stage on Flowering and Seed Setting of Rice in Panxi Region, Sichuan Province [J]. Acta Agron Sin, 2012, 38(09): 1734-1742.
[13] YU Chun-Hua, BIE Tong-De, WANG Cheng, ZHANG Xiao, WU Rong-Lin, CHENG Xiao-Ming, WANG Can-Guo, ZHAO Yun, CHENG Shun-He. Development of Near-isogenic Lines with Different Wheat Wx Genes and Their Effects on Amylose Content and Noodle Quality [J]. Acta Agron Sin, 2012, 38(03): 454-461.
[14] WU Hun-Lun, ZHAO Yuan, CHI De-Zhao. Relationship between Polymorphism at Wx Gene and Amylose Content in Hulless Barley [J]. Acta Agron Sin, 2012, 38(01): 71-79.
[15] ZHANG Hao, HUANG Zuan-Hua, WANG Jing-Chao, WANG Zhi-Qin, YANG Jian-Chang. Changes in Morphological and Physiological Traits of Roots and Their Relationships with Grain Yield during the Evolution of Mid-season Indica Rice Cultivars in Jiangsu Province [J]. Acta Agron Sin, 2011, 37(06): 1020-1030.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd