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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (11): 1715-1723.doi: 10.3724/SP.J.1006.2017.01715

• RESEARCH NOTES • Previous Articles     Next Articles

Development of PCR Functional Markers for Multiple Alleles of Wx and Their Application in Rice

MAO Ting1,2,LI Xu2,LI Zhen-Yu2,*,XU Zheng-Jin1,*   

  1. 1 Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, China; 2 Liaoning Province Saline and Alkaline Land Utilization and Research Institute, Panjin 124010, China
  • Received:2017-02-03 Revised:2017-07-23 Online:2017-11-12 Published:2017-08-01
  • Contact: 李振宇, E-mail: lyskjb@126.com; 徐正进, E-mail: xuzhengjin@126.com E-mail:chinamaoting1985@163.com
  • Supported by:

    This study was supported by the Cultivation Plan for Youth Agricultural Science Technology Innovative Talents of Liaoning Province (2015035, 2015036) and the Natural Science Foundation of China (31371587, 31430062).


Amylose content (AC) is one of the most important factors impacting rice eating quality, which was mainly determined by Wx locus. Multiple alleles, such as Wxa, Wxb, Wxin, and Wxmw contribute to the variation of AC in rice varieties. Selecting rice varieties with low-medium AC through Marker-assisted selection (MAS) is an important way to improve rice eating quality. However, distinguishing the Single Nucleotide Polymorphisms (SNP) of Wx alleles requires the DNA sequencing or restriction enzyme digestion, which is time consuming and labor-intensive. In this study, we developed effective MAS markers by tetra-primer ARMS-PCR technology utilizing functional SNPs corresponding to the different alleles, moreover we put forward corresponding solving schemes on low amplification efficiency and inaccuracy extension through adjusting the location of deliberate mismatch bases introduced. We easily differentiated the multiple alleles of Wx utilizing the markers Waxygt-ARMS2 and Waxyac-ARMS2, showing that these markers have significant application value with the features of rapid operation and low cost. We further analyzed the genotype distribution of Wx allele in Liaoning Province rice varieties, and found all of the forty tested varieties carried the Wxb allele with a narrow genetic diversity. The results of this study could provide theoretical basis and technical support on both new rice varieties breeding and rice eating quality improvement utilizing Wx alleles corresponding to low-medium AC by MAS in Liaoning Province.

Key words: Rice, Wx, Tetra-primer ARMS-PCR, Amylose content, Rice eating quality improvement

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