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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (03): 447-453.doi: 10.3724/SP.J.1006.2012.00447

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Mapping and Identification of QTLs for Fiber Micronaire on Chromosome 7 from Gossypium klotzschianum

XU Peng, ZHU Jing, ZHANG Xiang-Gui, NI Wan-Chao, XU Ying-Jun,SHEN Xin-Lian*   

  1. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2011-07-21 Revised:2011-10-12 Online:2012-03-12 Published:2012-01-09
  • Contact: 沈新莲, E-mail: Shenxinlian@yahoo.com.cn, Tel: 025-84390291

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Abstract: G. klotzschianum, carrying the elite alleles, is a diploid species of D genome originated from Galapagos Island, harboring the lethal genes to cause inconsistency of apical bud growth. In this research, we overcame the obstacle and established a BC1F2 population derived from (Simian2× G. klotzschianum) ×Simian2 (G. hirsutum). The SSR marker NAU1362 on chromosome 7 showed significant correlation with micronaire value by single marker analysis. The BC2F3 and BC2F4 populations were developed from the cross between BC1F2 individuals containing target segments of chromosome 7 from G. klotzschianum and recurrent parent Simian2. The software Cartographer (V2.5) and the composite interval mapping were further employed to identify quantitative trait loci (QTL) associated with fiber micronaire in two generations. The fiber micronaire QTL qFMIC-7-1 identified in BC1F2 population was confirmed in BC2F3 and BC2F4, which explained 9.0% and 8.8% of the phenotypic variance, respectively. The G. klotzschianum allele decreased the fiber micronaire value. Another micronaire QTL qFMIC-7-2 on chromosome 7 was also detected in BC2F3 and BC2F4 generations with phenotypic variance of 3.7% and 4.7%, respectively. Simian2 was genotyped as decreased micronaire value. This study provides valuable resources for effectively utilization of potential elite genes from G. klotzschianum.

Key words: Cotton, G. klotzschianum, Micronaire, QTL mapping

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