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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1716-1722.doi: 10.3724/SP.J.1006.2012.01716

• RESEARCH NOTES • Previous Articles     Next Articles

Differential Proteomics on Multi-Ovary and Mono-Ovary Trait of Wheat

WANG Zhi-Jun,MA Shou-Cai*,BI Xiao-Jing,SHI Xiu-Xiu,LI Qing-Feng,HAN Fang,QI Jia-Jia,WANG Shu-Ping,ZHANG Gai-Sheng,NIU Na   

  1. Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center / Yangling Branch of State Wheat Improvement Center / Wheat Breeding Engineering Research Center, Ministry of Education / Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling 712100, China?
  • Received:2012-02-16 Revised:2012-06-06 Online:2012-09-12 Published:2012-07-03
  • Contact: 马守才, E-mail: mashoucai@sohu.com, Tel: 13991218803

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Abstract:  Muliti-ovary is a favorable trait that might be a solution for increasing the yield of hybrid seed in wheat. In this study, the differential proteins of panicles at tetrad stage were detected between the mono-ovary line 77(2) and its near-isogenic multi-ovary line Mu77(2) using SDS-PAGE method. Approximately 450 protein spots were observed in the 2-DE gels within the area of pH 4–7 and molecular weight 14.4–97.4 kD, of which 30 were identified as differential protein spots with at least double expression levels and 99% statisticalty significant difference. Six specific protein spots with differential expressions were then analyzed using LC-MS/MS method. The result showed that they are glycine-rich RNA-binding protein (S1), SGT1-1 (S2), HMG-I/Y protein (S3), glutathione transferase (S4), fructose-bisphosphate aldolase (S5), and unnamed protein (S6). These proteins are involved in the regulation of DNA transcription, protein translation, energy metabolism, signal transduction, plants defense, and other resistance function, and might be related to the formation of multi-ovary in wheat.

Key words: Wheat, Multi-ovary trait, LC-MS/MS, Two-dimensional electrophoresis

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