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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (03): 471-478.doi: 10.3724/SP.J.1006.2012.00471

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Selection of Reference Genes from Sesame Infected by Macrophomina phaseolina

LIU Li-Ming1,2,3,LIU Hong-Yan1,2,3,*,TIAN Bao-Ming1   

  1. 1 Bioengineering Department, Zhengzhou University, Zhengzhou 450001, China; 2 Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 3 Henan Key Laboratory for Control of Crop Diseases and Insect Pests, Zhengzhou 450002, China
  • Received:2011-09-19 Revised:2011-12-15 Online:2012-03-12 Published:2012-01-04
  • Contact: 刘红彦, E-mail: liuhy1219@163.com, Tel: 0371-65730166

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Abstract: Stem rot caused by Macrophomina phaseolina (Tassi) Goid. is the most important disease in sesame, worldwide, the use of sesame cultivars resistant to stem rot provides an effective, economical, and environmentally friendly method to control the disease. The study on sesame defense response plays important roles in breeding resistant cultivars, andqRT-PCRwas an important method in studying plant defense response. In order to select the proper reference genes which can normalize expression of the target gene in sesame induced by Macrophomina phaseolina, nine genes 18S rRNA, NADHD, GAPDH, β-actin, α-tubulin, UBQ5, RPL4, eIF4A, and eEF1α, was analyzed by qRT-PCR. Among the nine genes, last seven were designed using CodeHop. BestKeeper, NormFinder and GeNorm analyses showed that UBQ5, eIF4A and α-tubulin were the top three optimum choices while eEF1α varied greatly. The analysis revealed that the three most stable genes UBQ5, eIF4A, α-tubulin were enough to obtain an accurate result when using multiple genes as control.

Key words: Sesame, Macrophomina phaseolina, qRT-PCR, Reference gene

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