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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1232-1239.doi: 10.3724/SP.J.1006.2012.01232

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Proteomic Analysis of Bud Differentiation between Cytoplasmic Male-Sterile Line and Maintainer in Tobacco

QI Jian-Min1,**,MA Hong-Bo1,2,**,XU Jian-Tang1,CHEN Mei-Xia1,3,ZHOU Dong-Xin4,WANG Tao5,CHEN Shun-Hui6   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 3 Ningde Normal University Biological Engineering, Ningde 352100, China; 4 Longyan Tobacco Branch Company, Longyan 364000, China; 5 Nanping Tobacco Company, Nanping 353200 China; 6 Fuzhou Institute of Tobacco Science of Fujian Province, Fuzhou 350003, China?
  • Received:2011-11-17 Revised:2012-02-22 Online:2012-07-12 Published:2012-04-06

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Abstract: To explorethe moleculargenetic mechanismsof cytoplasmic male sterility in tobacco,we studied the differential protein between the cytoplasmic male-sterile line and its maintainer. Immobilized pH gradient two-dimensional gel electrophoresis (2-DE) technique was used to separate the protein spots while gels were stained with the Coomassie Blue G-250. The difference between protein maps of bud from cytoplasmic male-sterile line MSK326 and maintainer line K326 was analyzed with PDQuest image software. Matrix-assisted laser-adsorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technique was used to obtain the peptide mass of differentially expressed protein spots. The Mascot software was used to search the protein database NCBInr to identify those spots interested. A total of 365 protein spots were detected within Mr 14.4–97.6 kD and pH 4–7. Seven protein spots appeared in the protein map of maintainer line K326 but absent in that of CMS line MSK326, which were identified as ribulose-1,5-bisphosphate carboxylase/oxygenase, polyphenol oxidase, patatin homolog, PSI 9 kD protein. It was inferred that the male sterility of MSK326 might be related to energy metabolism turbulence, abnormality in male organ development, deficient nutrition supply and immunity, and inhibition of energy transfer.

Key words: Tobacco, Cytoplasmic male sterility, Bud, Differential protein, 2-DE

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