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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1617-1624.doi: 10.3724/SP.J.1006.2012.01617

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Enhancement of Resistance to Fusarium Head Blight and Sharp Eyespot in Gastrodianin Transgenic Wheat

ZHOU Miao-Ping1,YANG Xue-Ming1,YAO Jin-Bao1,REN Li-Juan1,ZHANG Zeng-Yan2,MA Hong-Xiang1   

  1. 1 Provincial Key Laboratory for Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2 National Key Facility of Crop Gene Resources and Gene Improvement / Key Laboratory of Crop Genetic and Breeding, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2012-01-19 Revised:2012-04-20 Online:2012-09-12 Published:2012-07-03

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Abstract: Gastrodianin, also called Gastrodia antifungal protein (GAFP), can inhibit the growth of many fungal pathogens in vitro. The Gastrodianin gene driven by maize ubiquitin promoter in the transformation vector pAC-GAFP was introduced into wheat cultivars Yangmai 158 and Alondra via particle bombardment to investigate the resistance to fungal pathogens in transgenic wheat. A total of 14 transgenic lines were obtained and verified through PCR, FISH, and semiquantitative RT-PCR analyses. The results showed that the alien Gastrodianin gene was integrated into wheat genome in the transgenic lines and heritable to the offspring. The alien Gastrodianin gene was expressed at different levels in the transgenic lines of the homozygous T5 generation. The assessment of resistance to Fusarium graminearum and Rhizoctonia cerealis indicated that Gastrodianin suppressed the growth of pathogens in transgenic plants and reduced the severity of both diseases. The enhanced resistance degree was associated with the expression level of Gastrodianin gene in transgenic plants.

Key words: Wheat, Transformation, Gastrodianin, Fusarium head blight, Sharp eyespot

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