Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1607-1616.doi: 10.3724/SP.J.1006.2012.01607

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Functional Analysis of GmNF-YC2 Gene in Soybean (Glycine max)

CAO Yan1,ZHANG Xiao-Mei2,CHEN Xin-Jian1,*,FU Yong-Fu2,*   

  1. 1 College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China; 2 Key Laboratory of Soybean Biology / National Key Facility of Crop Gene Resource and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 10081, China
  • Received:2012-02-27 Revised:2012-04-20 Online:2012-09-12 Published:2012-07-03
  • Contact: 陈新建, E-mail: xinjian@371.net; 傅永福, E-mail: fufu19cn@163.com, Tel: 010-82105864

PDF

2209

Abstract: The eukaryotic nuclear factor Y (NF-Y) is a ubiquitous transcription factor complex composed of three distinct subunits (NF-YA, NF-YB, and NF-YC).NF-Y transcription factors typically act on the promoters of other regulatory factors to modulate gene expressions. Here, a NF-YC homologue, GmNF-YC2, was cloned from soybean variety KN18. The expression profiles checked by quantitative Real-time RT-PCR (qRT-PCR) showed that GmNF-YC2 was regulated by light but not by circadian clock. And GmNF-YC2 was expressed at the highest level in third trifoliolates at flowering. The results suggested that GmNF-YC2 was one of the important genes in the regulation of flowering in soybean. The transient expression in Arabidopsis protoplasts displayed that GmNF-YC2 protein was localized in nuclear section. It paved a way to study the function of NF-Y family in soybean and its application in soybean molecular breeding.

Key words: NF-Y transcription factor, Flowering, CONSTANS, Photoperiod, Glycine max

[1]Siefers N, Dang K K, Kumimoto R W, Bynum W E, Tayrose G, Holt B F. Tissue-specific expression patterns of Arabidopsis NF-Y transcription factors suggest potential for extensive combinatorial complexity. Plant Physiol, 2009, 149: 625-641

[2]Mantovani R. The molecular biology of the CCAAT-binding factor NF-Y. Gene, 1999, 239: 15-27

[3]Li X Y, Mantovani R, Hooft van Huijsduijnen R, Andre I, Benoist C, Mathis D. Evolutionary variation of the CCAAT-binding transcription factor NF-Y. Nucl Acids Res, 1992, 20: 1087-1091

[4]Maity S N, de Crombrugghe B. Role of the CCAAT-binding protein CBF/NF-Y in transcription. Trends Biochem Sci, 1998, 23: 174-178

[5]Cao S, Kumimoto R W, Siriwardana C L, Cao S, Kumimoto R W, Siriwardana C L, Risinger J R, Holt B F. Identification and characterization of NF-Y transcription factor families in the monocot model plant Brachypodium distachyon. PLoS One, 2011, 6(6): e21805

[6]Kwong R W, Bui A Q, Lee H, Kwong L W, Fischer R L, Goldberg R B, Harada J J. LEAFY COTYLEDON1-LIKE defines a class of regulators essential for embryo development. Plant Cell, 2003, 15: 5-18

[7]Lotan T, Ohto M, Yee K M, West M A, Lo R, Kwong R W, Yamagishi K, Fischer R L, Goldberg R B, Harada J J. Arabidopsis LEAFY COTYLEDON1 is sufficient to induce embryo development in vegetative cells. Cell, 1998, 93: 1195-1205

[8]Warpeha K M, Upadhyay S, Yeh J, Adamiak J, Hawkins S I, Lapik Y R, Anderson M B, Kaufman L S. The GCR1, GPA1, PRN1, NF-Y signal chain mediates both blue light and abscisic acid responses in Arabidopsis. Plant Physiol, 2007, 143: 1590-1600

[9]Nelson D E, Repetti P P, Adams T R, Creelman R A, Wu J, Warner D C, Anstrom D C, Bensen R J, Castiglioni P P, Donnarummo M G, Hinchey B S, Kumimoto R W, Maszle D R, Canales R D, Krolikowski K A, Dotson S B, Gutterson N, Ratcliffe O J, Heard J E. Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres. Proc Natl Acad Sci USA, 2007, 104: 16450-16455

[10]Li W X, Oono Y, Zhu J, He X J, Wu J M, Iida K, Lu X Y, Cui X, Jin H, Zhu J K. The Arabidopsis NFYA5 transcription factor is regulated transcriptionally and posttranscriptionally to promote drought resistance. Plant Cell, 2008, 20: 2238-2251

[11]Cai X, Ballif J, Endo S, Davis E, Liang M, Chen D, DeWald D, Kreps J, Zhu T, Wu Y. A putative CCAAT-binding transcription factor is a regulator of flowering timing in Arabidopsis. Plant Physiol, 2007, 145: 98-105

[12]Chen N Z, Zhang X Q, Wei P C, Chen Q J, Ren F, Chen J, Wang X C. AtHAP3b plays a crucial role in the regulation of flowering time in Arabidopsis during osmotic stress. J Biochem Mol Biol, 2007, 40: 1083-1089

[13]Ben-Naim O, Eshed R, Parnis A, Teper-Bamnolker P, Shalit A, Coupland G, Samach A, Lifschitz E. The CCAAT binding factor can mediate interactions between CONSTANS-like proteins and DNA. Plant J, 2006, 46: 462-476

[14]Kumimoto R W, Zhang Y, Siefers N, Holt B F. NF-YC3, NF-YC4 and NF-YC9 are required for CONSTANS-mediated, photoperiod-dependent flowering in Arabidopsis thaliana. Plant J, 2010, 63: 379-391

[15]Wenkel S, Turck F, Singer K, Gissot L, Le Gourrierec J, Samach A, Coupland G. CONSTANS and the CCAAT box binding complex share a functionally important domain and interact to regulate flowering of Arabidopsis. Plant Cell, 2006, 18: 2971-2984

[16]Liu J X, Howell S H. bZIP28 and NF-Y transcription factors are activated by ER stress and assemble into a transcriptional complex to regulate stress response genes in Arabidopsis. Plant Cell, 2010, 22: 782-796

[17]Yamamoto A, Kagaya Y, Toyoshima R, Kagaya M, Takeda S, Hattori T. Arabidopsis NF-YB subunits LEC1 and LEC1-LIKE activate transcription by interacting with seed-specific ABRE-binding factors. Plant J, 2009, 58: 843-856

[18]Combier J P, de Billy F, Gamas P, Niebel A, Rivas S. Trans-regulation of the expression of the transcription factor MtHAP2-1 by a uORF controls root nodule development. Genes Dev, 2008, 22: 1549-1559

[19]Combier J P, Frugier F, de Billy F, Boualem A, El-Yahyaoui F, Moreau S, Vernié T, Ott T, Gamas P, Crespi M, Niebel A. MtHAP2-1 is a key transcriptional regulator of symbiotic nodule development regulated by microRNA169 in Medicago truncatula. Genes Dev, 2006, 20: 3084-3088

[20]Zanetti M E, Blanco F A, Beker M P, Battaglia M, Aguilar O M. A C subunit of the plant nuclear factor NF-Y required for rhizobial infection and nodule development affects partner selection in the common bean-Rhizobium etli symbiosis. Plant Cell, 2010, 22: 4142-4157

[21]Stephenson T J, McIntyre C L, Collet C, Xue G P. TaNF-YC11, one of the light-upregulated NF-YC members in Triticum aestivum, is co-regulated with photosynthesis-related genes. Funct Integr Genom, 2010, 10: 265-276

[22]Ballif J, Endo S, Kotani M, MacAdam J, Wu Y. Over-expression of HAP3b enhances primary root elongation in Arabidopsis. Plant Physiol Biochem, 2011, 49: 579-583

[23]Redei G P. Supervital mutants of Arabidopsis. Genetics, 1962, 47: 443-60

[24]Koornneef M, Hanhart C J, van der Veen J H. A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana. Mol Gen Genet, 1991, 229: 57-66

[25]Putterill J, Robson F, Lee K, Simon R, Coupland G. The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell, 1995, 80: 847-857

[26]Valverde F, Mouradov A, Soppe W, Ravenscroft D, Samach A, Coupland G. Photoreceptor regulation of CONSTANS protein in photoperiodic flowering. Science, 2004, 303: 1003-1006

[27]Izawa T. Adaptation of flowering-time by natural and artificial selection in Arabidopsis and rice. J Exp Bot, 2007, 58: 3091-3097

[28]Jaeger K E, Wigge P A. FT protein acts as a long-range signal in Arabidopsis. Curr Biol, 2007, 17: 1050-1054

[29]Mathieu J, Warthmann N, Kuttner F, Schmid M. Export of FT protein from phloem companion cells is sufficient for floral induction in Arabidopsis. Curr Biol, 2007, 17: 1055-1060

[30]Tamaki S, Matsuo S,Wong H L, Yokoi S, Shimamoto K. Hd3a protein is a mobile flowering signal in rice. Science, 2007, 316: 1033-1036

[31]Kumimoto R W, Adam L, Hymus G J, Repetti P P, Reuber T L, Marion C M, Hempel F D, Ratcliffe O J. The nuclear factor Y subunits NF-YB2 and NF-YB3 play additive roles in the promotion of flowering by inductive long-day photoperiods in Arabidopsis. Planta, 2008, 228: 709-723

[32]An H, Roussot C, Suarez-Lopez P, Corbesier L, Vincent C, Pineiro M, Hepworth S, Mouradov A, Justin S, Turnbull C, Coupland G. CONSTANS acts in the phloem to regulate a systemic signal that induces photoperiodic flowering of Arabidopsis. Development, 2004, 131: 3615-3626

[33]Edwards D, Murray J A, Smith A G. Multiple genes encoding the conserved CCAAT-box transcription factor complex are expressed in Arabidopsis. Plant Physiol, 1998, 117: 1015-1022

[34]Peng Y, Jahroudi N. The NFY transcription factor functions as a repressor and activator of the von Willebrand factor promoter . Blood, 2002, 99: 2408-2417

[35]Peng Y, Jahroudi N. The NFY transcription factor inhibits von Willebrand factor promoter activation in non-endothelial cells through recruitment of histone deacetylases. J Biol Chem, 2003, 278: 8385-8394

[36]Chen Q J, Zhou H M, Chen J, Wang X C. Using a modified TAcloning method to create entry clones. Anal Biochem, 2006, 358: 120-125

[37]Hu R-B(胡瑞波). Molecular Cloning, Expression Profiles and Functional Analysis of FT/TFL1 Genes in Soybean (Glycine max). PhD Dissertation of Chinese Academy of Agricultural Sciences, 2009 (in Chinese with English Abstract)

[38]Frontini M, Imbriano C, Manni I, Mantovani R. Cell cycle regulation of NF-YC nuclear localization. Cell Cycl, 2004, 109: 217-222

[39]Kong F, Liu B, Xia Z, Sato S, Kim B M, Watanabe S, Yamada T, Tabata S, Kanazawa A, Harada K, Abe J. Two coordinately regulated homologs of FLOWERING LOCUS T are involved in the control of photoperiodic flowering in soybean. Plant Physiol, 2010, 154: 1220-1231

[40]Liu H, Wang H, Gao P, Xu J, Xu T, Wang J, Wang B, Lin C, Fu Y F. Analysis of clock gene homologs using unifoliolates as target organs in soybean (Glycine max). Plant Physiol, 2009, 166: 278-289

[41]Sun H, Jia Z, Cao D, Jiang B, Wu C, Hou W, Liu Y, Fei Z, Zhao D, Han T. GmFT2a, a soybean homolog of FLOWERING LOCUS T, is involved in flowering transition and maintenance. PLoS One, 2011, 6(12): e29238
[1] XU Xin, QIN Chao, ZHAO Tao, LIU Bin, LI Hong-Yu, LIU Jun. Function analysis of GmELF3s in regulating soybean flowering time and circadian rhythm [J]. Acta Agronomica Sinica, 2022, 48(4): 812-824.
[2] QIN Qin, TAO You-Feng, HUANG Bang-Chao, LI Hui, GAO Yun-Tian, ZHONG Xiao-Yuan, ZHOU Zhong-Lin, ZHU Li, LEI Xiao-Long, FENG Sheng-Qiang, WANG Xu, REN Wan-Jun. Characteristics of panicle stem growth and flowering period of the parents of hybrid rice in machine-transplanted seed production [J]. Acta Agronomica Sinica, 2022, 48(4): 988-1004.
[3] DONG Yan-Kun, HUANG Ding-Quan, GAO Zhen, CHEN Xu. Identification, expression profile of soybean PIN-Like (PILS) gene family and its function in symbiotic nitrogen fixation in root nodules [J]. Acta Agronomica Sinica, 2022, 48(2): 353-366.
[4] ZHANG Xue-Cui, SUN Su-Li, LU Wei-Guo, LI Hai-Chao, JIA Yan-Yan, DUAN Can-Xing, ZHU Zhen-Dong. Identification of resistance gene against phytophthora root rot in new soybean lines breeded in Henan province [J]. Acta Agronomica Sinica, 2021, 47(2): 275-284.
[5] ZHANG Xue-Cui,ZHONG Chao,DUAN Can-Xing,SUN Su-Li,ZHU Zhen-Dong. Fine mapping of Phytophthora resistance gene RpsZheng in soybean cultivar Zheng 97196 [J]. Acta Agronomica Sinica, 2020, 46(7): 997-1005.
[6] JIA Xiao-Ping,YUAN Xi-Lei,LI Jian-Feng,WANG Yong-Fang,ZHANG Xiao-Mei,ZHANG Bo,QUAN Jian-Zhang,DONG Zhi-Ping. Photo-thermal interaction model under different photoperiod-temperature conditions and expression analysis of SiCCT gene in foxtail millet (Setaria italica L.) [J]. Acta Agronomica Sinica, 2020, 46(7): 1052-1062.
[7] Fang-Quan WANG,Fang-Jun FAN,Shi-Jian XIA,Shou-Yu ZONG,Tian-Qing ZHENG,Jun WANG,Wen-Qi LI,Yang XU,Zhi-Hui CHEN,Yan-Jie JIANG,Ya-Jun TAO,Wei-Gong ZHONG,Jie YANG. Interactive effects of the photoperiod-/thermo-sensitive genic male sterile genes tms5 and pms3 in rice [J]. Acta Agronomica Sinica, 2020, 46(3): 317-329.
[8] Dai-Ling LIU,Jun-Feng XIE,Qian-Rui HE,Si-Wei CHEN,Yue HU,Jia ZHOU,Yue-Hui SHE,Wei-Guo LIU,Wen-Yu YANG,Xiao-Ling WU. QTL analysis for relative contents of glycinin and β-conglycinin fractions from storage protein in soybean seeds under monoculture and relay intercropping [J]. Acta Agronomica Sinica, 2020, 46(3): 341-353.
[9] LIU Hai-Dong, PAN Yun-Long, DU De-Zhi. Locus encryption for early flowering and QTL polymerization to create excellent early flowering resources of spring Brassica napus L. [J]. Acta Agronomica Sinica, 2020, 46(11): 1711-1721.
[10] ZHANG Shuang-Shuang,WANG Li-Wei,YAO Nan,GUO Guang-Yan,XIA Yu-Feng,BI Cai-Li. Expression of OsUBA and its function in promoting seed germination and flowering [J]. Acta Agronomica Sinica, 2019, 45(9): 1327-1337.
[11] WANG Yan-Hua,XIE Ling,YANG Bo,CAO Yan-Ru,LI Jia-Na. Flowering genes in oilseed rape: identification, characterization, evolutionary and expression analysis [J]. Acta Agronomica Sinica, 2019, 45(8): 1137-1145.
[12] CHEN Ying,ZHANG Sheng-Rui,WANG Lan,WANG Lian-Zheng,LI Bin,SUN Jun-Ming. Characteristics of oil components and its relationship with domestication of oil components in wild and cultivated soybean accessions [J]. Acta Agronomica Sinica, 2019, 45(7): 1038-1049.
[13] JIA Xiao-Ping,QUAN Jian-Zhang,WANG Yong-Fang,DONG Zhi-Ping,YUAN Xi-Lei,ZHANG Bo,LI Jian-Feng. Effects of different photoperiod conditions on agronomic traits of foxtail millet [J]. Acta Agronomica Sinica, 2019, 45(7): 1119-1127.
[14] Yun-Fu LI,Jing-Xian WANG,Yan-Fang DU,Hua-Wen ZOU,Zu-Xin ZHANG. Identification of indeterminate domain protein family genes associated with flowering time in maize [J]. Acta Agronomica Sinica, 2019, 45(4): 499-507.
[15] Hong-Ju JIAN,Bo YANG,Yang-Yang LI,Hong YANG,Lie-Zhao LIU,Xin-Fu XU,Jia-Na LI. Identification and expression analysis of PEBP gene family in oilseed rape [J]. Acta Agronomica Sinica, 2019, 45(3): 354-364.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd