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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1607-1616.doi: 10.3724/SP.J.1006.2012.01607

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Functional Analysis of GmNF-YC2 Gene in Soybean (Glycine max)

CAO Yan1,ZHANG Xiao-Mei2,CHEN Xin-Jian1,*,FU Yong-Fu2,*   

  1. 1 College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China; 2 Key Laboratory of Soybean Biology / National Key Facility of Crop Gene Resource and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 10081, China
  • Received:2012-02-27 Revised:2012-04-20 Online:2012-09-12 Published:2012-07-03
  • Contact: 陈新建, E-mail: xinjian@371.net; 傅永福, E-mail: fufu19cn@163.com, Tel: 010-82105864

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Abstract: The eukaryotic nuclear factor Y (NF-Y) is a ubiquitous transcription factor complex composed of three distinct subunits (NF-YA, NF-YB, and NF-YC).NF-Y transcription factors typically act on the promoters of other regulatory factors to modulate gene expressions. Here, a NF-YC homologue, GmNF-YC2, was cloned from soybean variety KN18. The expression profiles checked by quantitative Real-time RT-PCR (qRT-PCR) showed that GmNF-YC2 was regulated by light but not by circadian clock. And GmNF-YC2 was expressed at the highest level in third trifoliolates at flowering. The results suggested that GmNF-YC2 was one of the important genes in the regulation of flowering in soybean. The transient expression in Arabidopsis protoplasts displayed that GmNF-YC2 protein was localized in nuclear section. It paved a way to study the function of NF-Y family in soybean and its application in soybean molecular breeding.

Key words: NF-Y transcription factor, Flowering, CONSTANS, Photoperiod, Glycine max

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