净作和套作下大豆贮藏蛋白11S、7S组分相对含量的QTL分析

doi:10.3724/SP.J.1006.2020.94076

Abstract

Abstract:

The relative contents of glycinin and β-conglycinin from storage protein are closely related to the quality and function of soybean seeds. However, it is not clear whether or how glycinin and β-conglycinin contents change in maize-soybean strip intercropping system. The glycinin and β-conglycinin relative contents of the recombinant inbred lines (RILs) derived from the cross of ‘Nandou 12’ and ‘Jiuyuehuang’ were measured under different environments (E1: 2017, Renshou; E2: 2017, Ya’an; E3: 2016, Renshou) and planting patterns (M: monoculture; RI: relay intercropping), among which the differences were significant or extremely significant in parents and RILs. Based on a genetic linkage map with 6366 SNP markers, we detected ten QTLs for glycinin and β-conglycinin relative contents which were distributed in nine linkage groups with the phenotypic variation of 5.63%-9.68%. According to the soybean genomic information, 65 candidate genes were screened in the region of above-mentioned QTLs. These results lay a theoretical foundation for soybean quality breeding.

Key words: Soybean (Glycine max L.), storage protein, shade, QTL mapping

Dai-Ling LIU,Jun-Feng XIE,Qian-Rui HE,Si-Wei CHEN,Yue HU,Jia ZHOU,Yue-Hui SHE,Wei-Guo LIU,Wen-Yu YANG,Xiao-Ling WU. QTL analysis for relative contents of glycinin and β-conglycinin fractions from storage protein in soybean seeds under monoculture and relay intercropping[J].Acta Agronomica Sinica, 2020, 46(3): 341-353.

Figures/Tables 10

Table 1

Glycinin and β-conglycinin relative content of ‘Nandou 12’ (ND12) and ‘Jiuyuehuang’ (JYH) under different environments and planting patterns"

性状 Trait	种植方式 Planting pattern	E1			E2			E3
性状 Trait	种植方式 Planting pattern	南豆12 ND12	九月黄 JYH	Sig.	南豆12 ND12	九月黄 JYH	Sig.	南豆12 ND12	九月黄 JYH	Sig.
7S (%)	M	20.18±0.22 b	24.47±0.30 a	**	21.77±1.28 a	21.55±0.63 a	NS	22.84±0.20 a	22.96±0.47 a	NS
7S (%)	RI	23.42±1.40 a	25.25±2.05 a	NS	23.36±1.64 a	21.25±1.16 a	NS	22.43±2.21 a	22.21±1.45 a	NS
11S (%)	M	33.74±0.29 a	42.47±0.31 A	**	36.80±2.06 a	38.05±0.97 a	NS	37.99±0.69 a	36.08±0.76 a	*
11S (%)	RI	35.57±1.64 a	36.63±0.30 B	NS	38.09±1.37 a	35.35±1.02 b	*	33.30±3.60 a	38.12±2.03 a	NS
11S+7S (%)	M	53.92±0.51 b	66.94±0.27 a	**	58.58±3.33 a	59.60±1.59 a	NS	60.84±0.88 a	59.04±1.18 a	NS
11S+7S (%)	RI	58.99±3.04 a	61.88±2.16 b	NS	61.45±1.01 a	56.60±2.15 a	*	55.73±5.80 a	60.33±3.48 a	NS
11S/7S	M	1.67±0.00 a	1.74±0.03 A	*	1.69±0.01 a	1.77±0.01 a	**	1.66±0.02 A	1.57±0.02 B	**
11S/7S	RI	1.52±0.02 b	1.46±0.11 B	NS	1.64±0.16 a	1.67±0.05 b	NS	1.48±0.02 B	1.72±0.02 A	**
α' (%)	M	6.67±0.03 B	8.35±0.06 a	**	7.06±0.22 a	7.12±0.17 a	NS	7.08±0.54 a	8.01±0.16 A	*
α' (%)	RI	7.66±0.26 A	8.84±0.96 a	NS	7.29±0.83 a	6.74±0.83 a	NS	7.68±0.94 a	6.90±0.36 B	NS
Α (%)	M	6.84±0.18 a	9.49±0.28 a	**	7.92±0.75 a	7.63±0.69 a	NS	8.38±0.50 a	8.14±0.81 a	NS
Α (%)	RI	8.29±0.93 a	9.89±0.89 a	NS	8.50±0.92 a	7.25±0.15 a	NS	8.45±0.92 a	7.72±0.72 a	NS
Β (%)	M	6.67±0.20 B	6.63±0.42 a	NS	6.80±0.38 b	6.81±0.12 b	NS	7.39±0.91 a	6.81±0.30 b	NS
Β (%)	RI	7.47±0.23 A	6.52±0.66 a	NS	7.57±0.27 a	7.26±0.19 a	NS	6.30±0.60 a	7.59±0.37 a	*
A₃(%)	M	5.27±0.15 a	7.43±0.76 a	**	5.04±0.71 a	4.79±0.11 a	NS	5.61±0.23 a	5.28±1.24 a	NS
A₃(%)	RI	4.71±0.61 a	6.52±1.07 a	NS	5.47±0.21 a	4.65±0.31 a	*	5.37±0.15 a	6.00±0.46 a	NS
Acid (%)	M	13.01±0.88 a	17.17±0.78 a	**	15.79±2.58 a	14.68±1.07 a	NS	17.46±1.65 a	15.77±1.65 a	NS
Acid (%)	RI	14.14±0.65 a	16.35±2.29 a	NS	16.98±1.68 a	14.56±1.20 a	NS	13.72±1.33 b	17.39±0.44 a	**
Basic (%)	M	8.65±0.44 a	12.48±1.28 a	**	8.93±0.08 B	9.82±0.57 a	NS	8.27±0.47 a	8.66±0.11 a	NS
Basic (%)	RI	9.36±1.42 a	9.45±2.24 a	NS	9.90±0.15 A	9.24±0.96 a	NS	8.95±0.71 a	9.13±0.45 a	NS
A₅(%)	M	6.81±0.34 a	5.39±0.66 a	*	7.04±0.91 a	8.75±1.31 a	NS	6.71±2.71 a	6.37±1.59 a	NS
A₅(%)	RI	7.36±0.92 a	4.42±1.10 a	*	5.74±0.58 a	6.9±0.94 a	NS	5.27±2.86 a	5.60±1.65 a	NS

Table 1

Fig. 1

Fig. 2

Table 2

Statistic analysis of the glycinin and β-conglycinin relative content of seed storage protein in RILs population"

性状 Trait	环境 E	种植方式 Planting pattern	平均数±标准差 Mean±SD	最大值 Max.	最小值 Min.	极差 Range	变异系数 CV (%)	偏度 Skew.	峰度 Kurt.	P (S-W)
7S (%)	E1	M	22.34±1.81	30.03	19.73	10.30	8.11	1.73	4.07	0.00
	E1	RI	24.30±2.67	29.35	19.17	10.18	10.99	-0.10	-1.30	0.00
	E2	M	21.74±1.63	26.88	17.49	9.39	7.52	0.49	0.33	0.01
	E2	RI	22.44±1.45	26.24	18.16	8.08	6.45	0.03	0.06	0.74
	E3	M	24.67±1.92	29.92	19.60	10.32	7.78	0.23	-0.37	0.21
	E3	RI	25.22±1.92	30.18	19.89	10.29	7.60	0.14	-0.04	0.35
11S (%)	E1	M	36.16±2.68	42.62	30.03	12.59	7.42	0.14	-0.79	0.01
	E1	RI	35.65±2.27	41.83	29.24	12.59	6.37	0.15	-0.17	0.75
	E2	M	37.73±2.05	42.69	32.39	10.30	5.43	0.26	-0.35	0.05
	E2	RI	36.57±2.30	44.09	29.91	14.18	6.28	0.25	0.52	0.20
	E3	M	37.47±2.62	46.31	30.46	15.85	6.99	0.14	0.01	0.34
	E3	RI	36.22±2.74	44.36	30.87	13.49	7.56	0.81	0.48	0.00
11S+7S (%)	E1	M	58.50±2.81	65.08	52.05	13.03	4.80	-0.03	-0.79	0.00
	E1	RI	59.95±2.85	67.16	53.25	13.91	4.76	-0.01	-0.30	0.59
	E2	M	59.46±2.23	65.27	54.60	10.67	3.75	0.05	-0.74	0.04
	E2	RI	59.01±2.59	65.31	51.99	13.32	4.39	-0.04	0.01	0.30
	E3	M	62.15±2.65	72.35	55.68	16.67	4.27	0.21	0.28	0.10
	E3	RI	61.45±2.76	69.69	55.35	14.34	4.49	0.66	0.08	0.00
11S/7S	E1	M	1.63±0.19	2.10	1.10	1.00	11.67	-0.17	0.01	0.11
	E1	RI	1.49±0.22	2.03	1.06	0.97	14.67	0.29	-0.99	0.00
	E2	M	1.75±0.18	2.37	1.32	1.05	10.36	0.26	0.35	0.06
	E2	RI	1.64±0.16	2.08	1.26	0.82	9.46	0.31	-0.13	0.22
	E3	M	1.53±0.18	2.17	1.10	1.07	12.07	0.22	0.19	0.47
	E3	RI	1.45±0.18	1.93	1.09	0.84	12.32	0.30	-0.42	0.03
α' (%)	E1	M	7.45±0.79	9.99	6.10	3.89	10.59	1.19	1.71	0.00
	E1	RI	8.35±1.22	11.12	6.02	5.10	14.61	-0.07	-1.06	0.00
	E2	M	7.10±0.65	10.01	5.61	4.40	9.09	0.57	1.79	0.00
	E2	RI	7.43±0.61	9.87	5.81	4.06	8.18	0.24	1.72	0.00
	E3	M	8.39±0.77	10.46	6.66	3.80	9.21	0.42	-0.32	0.00
	E3	RI	8.53±0.85	11.05	5.98	5.07	9.95	0.01	0.41	0.14
α (%)	E1	M	8.12±0.73	11.71	6.81	4.90	9.00	1.91	5.37	0.00
	E1	RI	9.03±1.17	11.33	6.73	4.60	12.94	-0.17	-1.10	0.00
	E2	M	7.92±0.72	10.24	6.20	4.04	9.09	0.60	0.36	0.00
	E2	RI	8.04±0.63	9.81	6.32	3.49	7.87	-0.03	-0.02	0.61
	E3	M	9.28±0.89	11.93	6.81	5.12	9.59	0.22	-0.27	0.13
	E3	RI	9.43±0.94	12.60	6.97	5.63	9.98	0.29	0.47	0.14
β (%)	E1	M	6.77±0.58	9.48	5.31	4.17	8.54	1.72	5.30	0.00
	E1	RI	6.92±0.61	8.33	4.97	3.36	8.78	0.07	0.08	0.02
	E2	M	6.72±0.61	8.73	5.46	3.27	9.13	0.30	0.02	0.11
	E2	RI	6.97±0.60	8.95	5.49	3.46	8.63	0.46	0.80	0.01
	E3	M	7.01±0.76	9.37	5.23	4.14	10.85	0.11	-0.05	0.75
	E3	RI	7.26±0.59	8.71	5.54	3.17	8.15	0.03	0.09	0.64
A₃ (%)	E1	M	5.50±0.38	6.48	4.25	2.23	6.92	-0.28	0.39	0.21
	E1	RI	5.06±0.55	6.25	3.71	2.54	10.89	-0.41	-0.24	0.00
	E2	M	5.64±0.61	8.20	4.30	3.90	10.86	0.77	1.84	0.00
	E2	RI	5.35±0.54	7.02	3.16	3.86	10.11	-0.37	1.65	0.00
	E3	M	5.42±0.62	7.15	3.45	3.70	11.35	-0.36	0.34	0.04
	E3	RI	5.60±0.72	7.56	3.94	3.62	12.85	0.33	-0.14	0.10

Table 2

Table 3

Table 5

Table 6

Candidate gene classification related to glycinin and β-conglycinin relative content from storage protein"

分组 Group	生物过程 Biological process	基因ID (净作/套作) Gene ID (Monoculture/Relay intercropping)	注释信息 Annotation description
I	激素调节 Regulation of hormone	Glyma.01G179900; Glyma.14G193800, 060300, 195200, 195300, 196000, 196600, 197100, 197200, 197500, 197600, 198000, 198100, 198300, 198600, 199200, 200200, 202000, 202600, 202700, 203000 / Glyma.01G025300, 025400, 025500, 025600, 027400; Glyma.18G251800, 252200, 252300, 252400	脱落酸激活的信号通路、激素介导的信号通路、乙烯激活的信号通路、生长素反应、脱落酸反应 Abscisic acid-activated signaling pathway, hormone-mediated signaling pathway, ethylene-activated signaling pathway, response to auxin, response to abscisic acid
II	氨基酸合成 Amino acid synthesis	Glyma.01G180100; Glyma.04G211400; Glyma.06G243800; Glyma.14G195300, 196600, 197100, 198700, 202000, 202700, 203000, 203700 / Glyma.01G025600, 027100, 027400, 028100, 028700; Glyma.08G210500; Glyma.18G250600, 251800	蛋白丝氨酸/苏氨酸激酶活性、丝氨酸家族氨基酸代谢过程 Protein serine/threonine kinase activity, serine family amino acid metabolic process
III	蛋白质磷酸化 Protein phosphorylation	Glyma.06G243800; Glyma.14G195200, 196600, 202700, 203000 / Glyma.01G027100, 028000, 028700; Glyma.18G249400, 249700, 249900, 250500, 250600, 251800	蛋白质磷酸化、蛋白质丝氨酸/苏氨酸磷酸酶活性、蛋白质脱磷酸 Protein phosphorylation, protein serine/threonine phosphatase activity, protein dephosphorylation
IV	细胞器生物过程 Organelle biological process	Glyma.04G212200, 212400; Glyma.14G194300, 194500, 196600, 197600, 198000, 198100, 198600, 199000, 202000, 202400, 203000 / Glyma.01G025200, 026000, 026200, 026700, 028100; Glyma.18G248400, 251300, 251800, 253200	内质网、液泡、内质网膜 E ndoplasmic reticulum, vacuole, endoplasmic reticulum membrane
V	种子发育 Seed development	Glyma.01G179800; Glyma.14G197100, 197500, 199000, 202100 / Glyma.01G025200, 025300, 025400, 026200, 027400; Glyma.18G249400, 249700, 249800, 250000, 250100, 250600, 250700, 251200, 251800	子叶发育、以种子休眠结束的胚胎发育 Cotyledon development, embryo development ending in seed dormancy

Table 6

Table 7

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性状 Trait	环境 E	种植方式 Planting pattern	平均数±标准差 Mean±SD	最大值 Max	最小值 Min	极差 Range	变异系数 CV (%)	偏度 Skew.	峰度 Kurt.	P (S-W)
Acid (%)	E1	M	15.64±1.54	20.78	12.51	8.27	9.83	0.69	0.11	0.00
	E1	RI	14.38±1.52	17.99	8.70	9.29	10.57	-0.60	0.90	0.00
	E2	M	15.86±1.92	21.60	11.33	10.27	12.10	0.50	0.26	0.01
	E2	RI	15.16±1.68	19.19	9.57	9.62	11.08	-0.07	0.07	0.43
	E3	M	15.38±1.25	18.93	10.92	8.01	8.15	-0.29	0.98	0.04
	E3	RI	14.90±1.38	19.04	11.33	7.71	9.26	-0.19	-0.19	0.22
Basic (%)	E1	M	10.36±1.95	16.15	6.46	9.69	18.79	0.56	-0.26	0.00
	E1	RI	10.18±2.83	20.32	5.35	14.97	27.78	0.84	1.50	0.00
	E2	M	10.77±2.21	16.66	6.33	10.33	20.52	0.16	-0.62	0.03
	E2	RI	9.89±2.25	20.84	5.37	15.47	22.75	1.78	4.17	0.00
	E3	M	8.82±2.31	23.59	4.81	18.78	26.24	1.90	7.63	0.00
	E3	RI	8.09±1.79	15.48	4.50	10.98	22.09	0.96	2.47	0.00
A₅ (%)	E1	M	4.66±1.53	11.43	2.88	8.55	32.86	1.95	4.15	0.00
	E1	RI	6.02±2.32	15.45	0.88	14.57	38.44	1.21	1.57	0.00
	E2	M	5.45±1.63	11.61	3.01	8.60	29.84	1.22	1.56	0.00
	E2	RI	6.17±1.76	11.42	2.83	8.59	28.56	0.30	-0.21	0.00
	E3	M	7.85±3.36	17.74	1.09	16.65	42.85	0.65	-0.13	0.00
	E3	RI	7.64±3.94	18.83	1.75	17.08	51.52	1.03	0.31	0.00

性状 Trait	变异来源 Variability source
性状 Trait	E	P	G	E×P	E×G	P×G	E×P×G
7S (%)	2719.17^**	1130.16^**	16.35^**	198.05^**	12.31^**	15.24^**	9.05^**
11S (%)	232.79^**	390.23^**	9.34^**	22.11^**	9.01^**	8.57^**	6.90^**
11S+7S (%)	577.47^**	1.81	5.13^**	90.72^**	4.71^**	4.39^**	4.42^**
11S/7S	3509.46^**	3081.43^**	34.63^**	68.89^**	29.83^**	35.37^**	20.09^**
α' (%)	1534.95^**	670.80^**	10.08^**	169.27^**	6.65^**	8.86^**	6.62^**
α (%)	1726.56^**	423.291^**	8.68^**	181.27^**	6.97^**	7.90^**	5.07^**
β (%)	131.30^**	162.88^**	6.03^**	4.20^*	4.76^**	4.04^**	3.80^**
A₃ (%)	60.23^**	91.30^**	3.89^**	93.41^**	3.23^**	2.47^**	2.70^**
Acid (%)	43.73^**	324.46^**	5.05^**	26.16^**	3.87^**	3.09^**	4.02^**
Basic (%)	490.46^**	116.33^**	7.57^**	14.65^**	5.40^**	5.82^**	4.28^**
A₅ (%)	541.06^**	97.84^**	5.93^**	52.02^**	6.24^**	5.73^**	4.71^**

环境 Environ.	种植方式 Planting pattern	QTL	标记区间 Marker interval	LOD	贡献率 PVE(%)	加性效应 Add. effect	置信区间 Confidence interval
E1	M	qAcid-6-131	Marker359561-Marker361355	5.63	8.06	0.53	130.5-131.5
		qAcid-10-80	Marker584033-Marker584037	4.03	5.63	-0.46	79.5-80.5
		qAcid-14-155	Marker713356-Marker710168	4.06	5.81	-0.47	153.5-158.0
	RI	qGpC-18-85	Marker1158309-Marker1157431	5.61	6.62	1.06	84.5-85.5
	RI	qBeta-19-5	Marker1175558-Marker1175562	4.03	9.01	-0.18	4.5-5.5
E2	M	qBeta-1-135	Marker69562-Marker69768	3.21	7.49	-0.18	134.5-135.5
	RI	qAlpha-8-27	Marker460291-Marker460320	4.40	9.68	0.20	26.5-27.5
	RI	qBeta-1-13	Marker2130-Marker2656	3.22	7.14	-0.17	11.5-15.5
E3	M	qAcid-4-67	Marker270606-Marker271196	3.68	8.20	-0.40	66.5-70.5
E3	RI	qGpC-20-110	Marker1242459-Marker1242433	3.65	8.60	0.82	109.5-110.5

月 Month	旬 Ten-day periods	日平均温度 Daily mean temperature			日最高温度 Daily maximum temperature			日最低温度 Daily minimum temperature
月 Month	旬 Ten-day periods	E1	E2	E3	E1	E2	E3	E1	E2	E3
6月 June	上旬 The first ten days	25.40	23.13	25.80	30.47	28.02	31.86	21.59	19.87	20.99
	中旬 The middle ten days	23.94	22.68	27.40	28.07	26.92	32.54	21.12	19.82	22.43
	下旬 The last ten days	25.77	24.24	25.90	30.39	29.45	30.41	22.08	20.61	22.53
7月 July	上旬 The first ten days	27.83	26.09	27.20	33.93	31.38	31.26	23.67	22.73	23.57
	中旬 The middle ten days	29.23	27.78	27.80	35.00	34.31	32.80	24.45	23.50	23.20
	下旬 The last ten days	30.41	28.08	27.90	36.13	35.23	32.84	25.88	24.15	24.23
8月 August	上旬 The first ten days	29.41	28.11	27.00	35.17	34.20	31.98	25.55	24.43	23.79
	中旬 The middle ten days	27.71	26.10	30.40	32.25	31.20	35.66	24.23	23.24	26.33
	下旬 The last ten days	26.16	24.36	28.20	29.99	28.63	33.05	23.40	21.88	24.72
9月 September	上旬 The first ten days	23.95	22.54	23.10	27.66	27.38	27.12	21.31	19.81	20.43
	中旬 The middle ten days	23.68	22.29	22.00	27.40	26.03	25.40	21.22	20.30	19.82
	下旬 The last ten days	23.04	21.55	22.30	26.43	25.89	25.95	20.94	19.24	19.88
10月 October	上旬 The first ten days	19.97	18.77	22.00	23.73	23.03	25.59	17.70	16.85	19.47
	中旬 The middle ten days	17.07	15.75	18.30	19.41	18.36	20.94	15.65	14.28	16.55
	下旬 The last ten days	16.02	14.97	17.70	18.60	17.70	20.75	14.33	13.58	15.76

QTL analysis for relative contents of glycinin and β-conglycinin fractions from storage protein in soybean seeds under monoculture and relay intercropping

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