增加穗粒数的水稻染色体代换系Z747鉴定及相关性状QTL定位

doi:10.3724/SP.J.1006.2020.92022

Abstract

Abstract:

Increasing grain number per panicle is important for rice breeding for high yield. Its inheritance is very complex and controlled by many genes. Chromosome segment substitution lines can dissect complex traits controlled by many genes, and thus are ideal genetic research materials. Here, an excellent rice chromosome segment substitution line Z747 with increased grain number was identified from recipient Nipponbare and donor Xihui 18 through advanced backcrossing and inbreeding combined SSR marker-assisted selection. Z747 carried fifteen substitution segments with 4.49 Mb of average length. Compared with Nipponbare, Z747 had significantly increased spikelet number per panicle, number of primary branches, number of secondary branches, panicle length and grain length, and decreased grain width and seed setting rate. However, the seed setting rate in Z747 was still up to 81%. Furthermore, secondary F₂ population from crosses between Nipponbare and Z747 was used to map QTL for related traits. A total of 46 QTLs distributed on chromosomes 1, 2, 3, 5, 6, 9, 11, and 12 were detected. Among them, 12 QTLs such as qGPP12, qPH-3-1, and qPH-3-2 etc. might be alleles of cloned genes, and the remaining 34 QTLs such as qSPP9 etc. might not be identified in the past. The spikelet number per panicle of Z747 was mainly controlled by two QTLs (qSPP3 and qSPP5) with effects of increasing spikelet number and one (qSPP9) with decreasing effects. These results are important for fine mapping and cloning of major QTL, and developing single-segment substitution lines carrying favorable QTLs.

Key words: rice, chromosome segment substitution lines, grain number, QTL mapping

WANG Da-Chuan,WANG Hui,MA Fu-Ying,DU Jie,ZHANG Jia-Yu,XU Guang-Yi,HE Guang-Hua,LI Yun-Feng,LING Ying-Hua,ZHAO Fang-Ming. Identification of rice chromosome segment substitution Line Z747 with increased grain number and QTL mapping for related traits[J].Acta Agronomica Sinica, 2020, 46(01): 140-146.

Figures/Tables 4

Fig. 1

Fig. 2

Table 1

Table 2

QTL for rice agronomically important traits detected in Z747"

性状 Trait	QTL	染色体 Chr.	连锁标记 Linked marker	加性效应 Additive effect	贡献率 Var. (%)	P值 P-value	可能的等位基因 Possible alleles
株高	qPH-3-1	3	RM5474	-7.79	9.45	0.0004	OsRLCK102^[20]
Plant height	qPH-3-2	3	RM3417	5.74	5.15	0.0003	HTD2^[21]
	qPH-3-3	3	RM6266	5.03	10.30	<0.0001	—
	qPH5	5	RM5874	13.67	27.54	0.0003	—
	qPH11	11	RM7120	5.31	4.39	0.0090	—
穗长	qPL3	3	RM6266	0.51	2.70	0.0020	—
Panicle length	qPL11	11	RM7120	1.28	6.61	0.0012	—
一次枝梗数	qNPB2	2	RM1385	0.59	6.07	0.0002	Pbr2^[22]
Number of primary branch	qNPB3	3	RM3766	0.83	7.45	0.0012	—
	qNPB9	9	RM24537	1.79	9.96	<0.0001	—
	qNPB12	12	RM247	-1.07	8.14	0.0049	—
二次枝梗数	qNSB2	2	RM6378	2.33	1.65	0.0004	—
Number of secondary branch	qNSB3	3	RM3766	3.86	7.83	0.0005	—
	qNSB5	5	RM18067	3.59	5.92	0.0036	JMJ703^[23]
	qNSB6	6	RM494	-2.46	7.10	0.0004	—
	qNSB-9-1	9	RM7048	-7.39	20.71	0.0007	—
	qNSB-9-2	9	RM24537	6.29	6.05	0.0001	—
实粒数	qGPP2	2	RM1385	8.89	4.53	0.0014	—
Grain number per panicle	qGPP-9-1	9	RM7048	-28.08	19.83	0.0008	—
	qGPP-9-2	9	RM24537	18.49	3.66	0.0038	—
	qGPP12	12	RM3331	-18.64	8.21	<0.0001	ND1^[24]
总粒数	qSPP3	3	RM3766	20.73	9.66	0.0002	—
Spikelet number per panicle	qSPP5	5	RM18067	20.07	7.90	0.0015	—
	qSPP9	9	RM7048	-29.51	14.15	0.0044	—
结实率	qSSR1	1	RM259	-24.38	11.97	0.0001	DFO1^[25]
Seed setting rate	qSSR2	2	RM1385	4.65	1.19	0.0069	—
	qSSR-3-1	3	RM5474	18.66	9.82	<0.0001	OsMCA1^[26]
	qSSR-3-2	3	RM3766	-10.38	3.58	0.0014	OsFRDL1^[27]
	qSSR5	5	RM5874	42.72	46.67	<0.0001	PTB1^[28]
	qSSR6	6	RM5371	-17.59	6.48	0.0013	DCM1^[29]
	qSSR-9-1	9	RM7048	-20.74	10.33	0.0004	—
	qSSR-9-2	9	RM24537	23.28	5.24	<0.0001	—
	qSSR12	12	RM3331	-14.58	4.80	<0.0001	—
粒长	qGL3	3	RM6266	0.40	63.73	<0.0001	—
Grain length	qGL5	5	RM18067	0.24	9.29	0.0007	—
	qGL12	12	RM3331	0.16	3.21	0.0047	—
粒宽	qGW2	2	RM1385	-0.07	5.59	0.0080	OsVPE3^[30]
Grain width	qGW5	5	RM18148	-0.08	6.05	0.0029	—
长宽比	qRLW2	2	RM1385	0.08	5.35	0.0004	—
Rate of length to width	qRLW-3-1	3	RM3766	0.10	5.42	0.0047	—
	qRLW-3-2	3	RM6266	0.13	19.74	<0.0001	—
	qRLW5	5	RM18148	0.13	10.17	0.0002	—
	qRLW6	6	RM494	-0.09	10.31	<0.0001	—
	qRLW12	12	RM3331	0.15	7.70	<0.0001	—
千粒重	qGWT2	2	RM1385	-0.80	4.01	0.0048	OsVPE3^[30]
1000-kernel weight	qGWT3	3	RM6266	1.82	28.67	<0.0001	—

Table 2

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性状 Trait	平均值±标准差(亲本) Mean±SD (parents)		F₂群体 F₂ population
性状 Trait	日本晴 Nipponbare	Z747	平均值±标准差 Mean±SD	范围 Range	偏度 Skewness	峰度 Kurtosis
株高 Plant height (cm)	88.1±3.4	91.8±6.9	91.9±8.2	68.0-130.8	0.39	0.31
穗长Panicle length (cm)	19.7±1.2	23.3±1.3^**	19.3±1.6	14.8-27.3	-0.18	0.57
有效穗数Panicle number	19.3±5.1	15.5±6.4	15.6±5.5	3.0-36.0	0.40	-0.03
一次枝梗数Number of primary branch	7.4±0.5	12.2±1.3^**	8.5±1.1	6.9-13.4	-0.15	0.27
二次枝梗数Number of secondary branch	14.3±1.7	40.5±1.2^**	15.1±4.3	7.4-45.6	0.03	0.71
每穗总粒数Spikelet number per panicle	110.7±16.4	173.0±49.6^**	94.3±19.9	21.9-221.7	-0.19	0.39
每穗实粒数Grain number per panicle	101.4±16.5	139.8±41.2	79.4±15.6	10.1-142.5	-0.23	0.65
结实率Seed setting rate (%)	91.0±2.0	81.0±0.7^**	85.1±12.9	10.9-96.3	-2.17	10.44
粒长Grain length (mm)	7.17±0.24	7.93±0.04^**	7.45±0.47	6.61-10.05	0.36	1.81
粒宽Grain width (mm)	3.40±0.08	3.23±0.05^*	3.34±0.13	2.38-3.52	-0.38	0.26
千粒重1000-grain weight (g)	24.6±1.6	25.5±0.7	25.5±1.8	21.0-32.3	0.28	1.27
单株产量Yield per plant (g)	38.6±9.8	40.9±5.3	31.7±12.4	2.4-72.5	0.45	0.20

Identification of rice chromosome segment substitution Line Z747 with increased grain number and QTL mapping for related traits

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