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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (01): 52-61.doi: 10.3724/SP.J.1006.2020.91024


QTL mapping for total grain anthocyanin content and 1000-kernel weight in barley recombinant inbred lines population

YANG Xiao-Meng1,2,LI Xia1,2,PU Xiao-Ying1,2,DU Juan1,2,Muhammad Kazim Ali3,YANG Jia-Zhen1,2,ZENG Ya-Wen1,2,*(),YANG Tao1,2   

  1. 1 Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China
    2 Agricultural Biotechnology Key Laboratory of Yunnan Province, Kunming 650205, Yunnan, China
    3 Department of Biotechnology, Fedural Urdu University of Arts, Science and Technology, Karachi 75300, Pakistan
  • Received:2019-03-18 Accepted:2019-08-09 Online:2020-01-12 Published:2019-09-18
  • Contact: Ya-Wen ZENG E-mail:zengyw1967@126.com
  • Supported by:
    This study was supported by Yunnan Applied Basic Research Projects of China(2017FD021);China Agriculture Research System(CARS-05-01A-04)


In this study, 193 recombinant inbred lines (RILs) derived from a cross between Ziguangmangluoerleng (purple barley, endemic to Yunnan, China) and Schooner (yellow barley introduced from Australia), grown at three sites of Yunnan province of China for three consecutive years (2013-2015), were used to determine total grain anthocyanin content, 1000-kernel weight and their correlations, and mapped QTLs. There was a significant negative correlation between total grain anthocyanin content and 1000-kernel weight. Twelve QTLs for total grain anthocyanin content were located on chromosomes 1H, 2H, 4H, 6H, and 7H which showed 5.06% to 23.86% of phenotypic variation. Eight QTLs for 1000-kernel weight were located on chromosomes 2H, 4H, and 7H, which explained phenotypic variation from 4.67% to 42.32%. Ten QTLs had phenotypic variation ≥10% and five QTLs ≥ 20%, while the highest phenotypic variation was 42.32%. In addition, two QTLs for total grain anthocyanin content were repeatedly detected at two sites for two years, and located on 2H Bmag0125 to GBM1309 and 7H EBmatc0016 to Bmag0206 interval, respectively, which accounted for 13.66% to 17.76%, and 13.07% to 16.43% of the phenotypic variation, respectively. Two QTLs for 1000-kernel weight were repeatedly detected at three sites for three years and located on 2H scssr03381 to scssr07759 and 7H GBM1297 to GBM1303 interval, which showed 4.67% to 14.55% and 34.51% to 42.32% of phenotypic variations, respectively, and their contribution rates of additive effects were consistent. The common major QTLs for two agronomic attributes were mainly distributed on chromosomes 2H and 7H. These results provide a basis for further fine mapping, cloning and marker-assisted breeding of beneficial genes related to total grain anthocyanin content and 1000-kernel weight.

Key words: barley RIL, total anthocyanin content, 1000-kernel weight, correlation, QTL mapping

Table 1

Phenotypic values of total grain anthocyanin content and 1000-kernel weight in barley "

Site and year
亲本Parents RIL群体 RIL population
Schooner 平均值
CV (%)
TAC (mg g-1)
玉溪 Yuxi, 2013 0.96 0.48 0.65 C 0.22 0.36-1.38 33.85 1.22 0.75
白邑 Baiyi, 2014 1.03 0.63 0.79 B 0.21 0.50-1.50 26.58 1.33 1.09
嵩明 Songming, 2015 1.14 0.70 0.84 A 0.21 0.50-1.58 25.00 1.14 0.81
TKW (g)
玉溪 Yuxi, 2013 35.50 46.00 45.55 C 6.01 31.65-58.00 13.19 -0.34 -0.62
白邑 Baiyi, 2014 33.35 42.55 44.00 B 6.64 23.05-60.50 15.09 -0.28 -0.35
嵩明 Songming, 2015 31.00 40.05 42.36 A 7.53 25.00-57.60 17.78 -1.10 4.00

Fig. 1

Frequency distribution of total grain anthocyain content and 1000-kernel weight in barley The abscissa represents total grain anthocyain content and 1000-kernel weight in barley RIL population grown at three sites (Yuxi, Baiyi, Songming) for three consecutive years (2013-2015), respectively; ordinate represents number of lines in total grain anthocyain content and 1000-kernel weight, respectively."

Table 2

Correlation coefficient between total grain anthocyanin content and 1000-kernel weight in barley "

Site and year
总花色苷含量 Total grain anthocyonin content 千粒重 1000-kernel weight
Yuxi 2013
Baiyi 2014
Songming 2015
Yuxi 2013
Baiyi 2014
Songming 2015
玉溪Yuxi 2013 1.00
白邑Baiyi 2014 0.918** 1.00
嵩明Songming 2015 0.873** 0.833** 1.00
玉溪Yuxi 2013 -0.223** -0.151* -0.233** 1.00
白邑Baiyi 2014 -0.163* -0.130 -0.174* 0.832** 1.00
嵩明Songming 2015 -0.146* -0.064 -0.173* 0.775** 0.720** 1.00

Table 3

QTL analysis for total grain anthocyonin content and 1000-kernel weight of barley "

Site and year
QTL 连锁群
Marker interval
LOD value
Contribution (%)
玉溪 Yuxi
qTAC-1H 1H GBM1234-Bmag0504 4.04 5.91 0.055
qTAC-2H 2H Bmag0125-GBM1309 10.42 13.66 0.080
qTAC-4H 4H HVBAMMGB84-BMAG0808 5.21 6.23 -0.054
qTAC-6H 6H GBMS0072-GBM1270 4.06 7.68 0.061
qTAC-7H 7H EBmatc0016-Bmag0206 9.82 13.07 -0.079
qTKW-2H 2H Scssr03381-scssr07759 7.54 14.55 -2.293
qTKW-4H 4H Scssr20569-Bmag0781 3.81 4.73 1.396
qTKW-7H 7H GBM1297-GMS056 26.14 42.32 -4.390
白邑 Baiyi
qTAC-2H 2H Bmag0125-GBM1309 11.38 17.76 0.089
qTAC-4H 4H GBM1221-GBM1501 3.58 5.06 0.049
qTAC-6H 6H BMAG0807-GBM1212 4.08 6.49 0.054
qTAC-7H 7H EBmatc0016-Bmag0206 9.24 16.43 -0.085
qTKW-2Ha 2H Scssr03381-scssr07759 2.75 4.67 -1.434
qTKW-2Hb 2H Bmag0749-GBMS0128 4.59 7.70 -1.839
qTKW-7H 7H GMS056-GBM1303 18.73 34.51 -4.250
嵩明 Songming 2015 总花色苷
qTAC-2H 2H GBM1121-Bmag0125 12.16 20.09 0.095
qTAC-6H 6H GBM1212-Bmag0173 3.77 6.24 0.053
qTAC-7H 7H GBM1516-EBmatc0016 12.01 23.86 -0.104
qTKW-2H 2H Scssr03381-scssr07759 2.50 5.39 -1.599
qTKW-7H 7H GMS056-GBM1303 17.37 35.37 -4.569

Fig. 2

Distribution of QTLs for total grain anthocyonin content and 1000-kernel weight of barley Triangle pattern indicates QTL for total anthocyanin content (TAC), pentagram pattern indicates QTL for 1000-kernel weight (TKW). The white patterns, gray patterns and black patterns represent the QTL loci detected in Yuxi (2013), Baiyi (2014), and Songming (2015), respectively. The physical positions corresponding to some molecular markers on 2H and 7H are indicated on the right side of the Figure."

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