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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (05): 789-805.doi: 10.3724/SP.J.1006.2013.00789

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Analysis of MAPK7 Gene Family and Their Promoters from Brassica napus

ZHU Bin1,2,3,**,LU Jun-Xing1,2,3,**,PENG Qian1,2,3,WENG Chang-Mei1,2,3,WANG Shu-Wen1,2,3,YU Hao1,2,3,LI Jia-Na1,2,3,LU Kun1,2,3,*,LIANG Ying1,2,3,*   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; 2 Chongqing Rapeseed Engineering & Technology Research Center, Chongqing 400715, China; 3Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
  • Received:2012-11-01 Revised:2013-01-15 Online:2013-05-12 Published:2013-02-19
  • Contact: 梁颖, E-mail: yliang@swu.edu.cn; 卢坤, E-mail: drlukun@swu.edu.cn

Abstract:

Mitogen-activated protein kinase (MAPK) is a large family of serine/threonine protein kinase, containing 11 conserved subdomains. MAPK pathway play a central role in transferring information from diverse receptors/sensors to a wide range of cellular responses in plants. Signaling through MAP kinase cascade can lead to cellular responses including cell division, development, hormone, physiology, as well as the response to a broad variety of biotic and abiotic stresses. In this study, the full-length cDNA of MAPK7 gene family was isolated from Brassica napus. There were three members in this gene family, including BnMAPK7-1, BnMAPK7-2, and BnMAPK7-3. Standard mRNA lengths of these genes were 1 593, 1 434 and 1 547 bp. Phylogenetic tree showed that BnMAPK7 gene family was all originated from Brassica rapa, and BnMAPK7-1/-2 were generated from ancestral gene of Bra03723, while BnMAPK7-3 was evolved from ancestral gene of Bra03724. We also cloned promoters of BnMAPK7-1/-2, which contain some light responsive elements, hormone responsive elements and stress response elements. Quantitative real-time PCR (qRT-PCR) showed that BnMAPK7 gene family expressed in all organs tested, and could be induced by phytohormones (MeJA, ABA, and SA), signaling molecules (H2O2), stress (heat) and injury (wounding and Sclerotinia sclerotiorum). This study preliminarily proved that the BnMAPK7 gene family plays certain roles in plant stress responses. In addition, the recombinant BnMAPK7-1/-3 proteins were successfully expressed in Escherichia coli, but the main form of proteins was inclusion body, this result would contribute to further discuss in the future.

Key words: Brassica napus, MAPK7 gene, Quantitative real-time PCR, Prokaryotic expression

[1]Rohila J S, Yang Y N. Rice mitogen-activated protein kinase gene family and its role in biotic and abiotic stress response. J Integr Plant Biol, 2007, 49: 751–759



[2]Jonak C, Okresz L, Bogre L, Hirt H. Complexity, cross talk and integration of plant MAP kinase signalling. Curr Opin Plant Biol, 2002, 5: 415–424



[3]Ichimura K, Shinozaki K, Tena G, Sheen J, Henry Y, Champion A, Kreis M, Zhang S Q, Hirt H, Wilson C, Heberle-Bors E, Ellis B E, Morris P C, Innes R W, Ecker J R, Scheel D, Klessig D F, Machida Y, Mundy J, Ohashi Y, Walker J C, Grp M. Mitogen-activated protein kinase cascades in plants: a new nomenclature. Trends Plant Sci, 2002, 7: 301–308



[4]Decroocqferrant V, Decroocq S, Vanwent J, Schmidt E, Kreis M. A Homolog of the map/erk family of protein-kinase genes is expressed in vegetative and in female reproductive-organs of petunia-hybrida. Plant Mol Biol, 1995; 27: 339–350



[5]Mizoguchi T, Hayashida N, Yamaguchishinozaki K, Kamada H, Shinozaki K. Atmpks: a gene family of plant map kinases in Arabidopsis thaliana. Febs Lett, 1993, 336: 440–444



[6]Mizoguchi T, Gotoh Y, Nishida E, Yamaguchishinozaki K, Hayashida N, Iwasaki T, Kamada H, Shinozaki K. Characterization of 2 cDNAs that encode map kinase homologs in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase-activities in cultured-cells. Plant J, 1994, 5: 111–122



[7]Mizoguchi T, Irie K, Hirayama T, Hayashida N, YamaguchiShinozaki K, Matsumoto K, Shinozaki K. A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana. Proc Nat Acad Sci USA, 1996, 93: 765–769



[8]Wilson C, Eller N, Gartner A, Vicente O, Heberlebors E. Isolation and characterization of a tobacco cdna clone encoding a putative map kinase. Plant Mol Biol, 1993, 23: 543–551



[9]Vogel J T, Zarka D G, van Buskirk H A, Fowler S G, Thomashow M F. Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabidopsis. Plant J, 2005, 41: 195–211



[10]Ortiz-Masia D, Perez-Amador M A, Carbonell J, Marcote M J. Diverse stress signals activate the C1 subgroup MAP kinases of Arabidopsis. FEBS Lett, 2007, 581: 1834–1840



[11]Ortiz-Masia D, Perez-Amador M A, Carbonell P, Aniento F, Carbonell J, Marcote M J. Characterization of PsMPK2, the first C1 subgroup MAP kinase from pea (Pisum sativum L.). Planta, 2008, 227: 1333–1342



[12]Chen X, Truksa M, Shah S, Weselake R J. A survey of quantitative real-time polymerase chain reaction internal reference genes for expression studies in Brassica napus. Anal Biochem, 2010, 405: 138–140



[13]Bustin S A, Benes V, Garson J A, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl M W, Shipley G L, Vandesompele J, Wittwer C T. Primer sequence disclosure: a clarification of the MIQE guidelines. Clin Chem, 2011, 57: 919–921



[14]Ding Y(丁勇), Chang W(常玮), Liu X-Z(刘小烛). Molecular cloning, expression vector construction and prokaryotic expression of BnClo1 gene from Brassica napus. Sci Agric Sin (中国农业科学), 2010; 43(2): 252–258 (in Chinese with English abstract)



[15]Parkin I A P, Sharpe A G, Lydiate D J. Patterns of genome duplication within the Brassica napus genome. Genome, 2003, 46: 291–303



[16]Piquemal J, Cinquin E, Couton F, Rondeau C, Seignoret E, Doucet I, Perret D, Villeger M J, Vincourt P, Blanchard P. Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers. Theor Appl Genet, 2005, 111: 1514–1523



[17]Inaba R, Nishio T. Phylogenetic analysis of Brassiceae based on the nucleotide sequences of the S-locus related gene, SLR1. Theor Appl Genet, 2002, 105: 1159–1165



[18]OECD/GD(97)63. Series on Harmonization of Regulatory Oversight in Biotechnology No.7: Consensus document on the biology of Brassica napus L.(Oilseed rape). Paris: Organisation for Economic Co-Operation and Development, 1997. pp 1–32 (http://www.oecd.org/science/biotrack/27531440. pdf)



[19]Lysak M A, Koch M A, Pecinka A, Schubert I. Chromosome triplication found across the tribe Brassiceae. Genome Res, 2005, 15: 516–525



[20]Higgins R, Lockwood T, Holley S, Yalamanchili R, Stratmann J W. Changes in extracellular pH are neither required nor sufficient for activation of mitogen-activated protein kinases (MAPKs) in response to systemin and fusicoccin in tomato. Planta, 2007, 225: 1535–1546



[21]Ichimura K, Mizoguchi T, Yoshida R, Yuasa T, Shinozaki K. Various abiotic stresses vapidly activate Arabidopsis MAP kinases ATMPK4 and ATMPK6. Plant J, 2000, 24: 655–665



[22]Katou S, Kuroda K, Seo S, Yanagawa Y, Tsuge T, Yamazaki M, Miyao A, Hirochika H, Ohashi Y. A calmodulin-binding mitogen-activated protein kinase phosphatase is induced by wounding and regulates the activities of stress-related mitogen-activated protein kinases in rice. Plant Cell Physiol, 2007, 48: 332–344



[23]Usami S, Banno H, Ito Y, Nishihama R, Machida Y. Cutting activates a 46-kilodalton protein-kinase in plants. Proc Nat Acad Sci USA, 1995, 92: 8660–8664



[24]Zhang S Q, Klessig D F. The tobacco wounding-activated mitogen-activated protein kinase is encoded by SIPK. Proc Nat Acad Sci USA, 1998, 95: 7225–7230



[25]Devoto A, Ellis C, Magusin A, Chang H S, Chilcott C, Zhu T, Turner J G. Expression profiling reveals COI1 to be a key regulator of genes involved in wound- and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions. Plant Mol Biol, 2005, 58: 497–513



[26]Leon J, Rojo E, Sanchez-Serrano J J. Wound signalling in plants. J Exp Bot, 2001, 52: 1–9



[27]Turner J G, Ellis C, Devoto A. The jasmonate signal pathway. Plant Cell, 2002, 14: S153–S164



[28]Yin H(尹恒), Yang J-L(杨金丽), Li S-G(李曙光), Zhao X-M(赵小明), Bai X-F(白雪芳), Ma X-J(马小军), Du Y-G(杜昱光). Cloning and analysis of BnMPK4, a novel MAP kinase gene induced by oligochitosan in Brassica napus. Acta Agron Sin (作物学报), 2008, 34(5): 743−747 (in Chinese with English abstract)



[29]Guan L Q M, Zhao J, Scandalios J G. Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H2O2 is the likely intermediary signaling molecule for the response. Plant J, 2000, 22: 87–95



[30]Pei Z M, Murata Y, Benning G, Thomine S, Klusener B, Allen G J, Grill E, Schroeder J I. Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature, 2000, 406: 731–734



[31]Rojo E, Solano R, Sanchez-Serrano J J. Interactions between signaling compounds involved in plant defense. J Plant Growth Regul, 2003, 22: 82–98



[32]Wang Z, Mao H, Dong C H, Ji R Q, Cai L, Fu H, Liu S Y. Overexpression of Brassica napus MPK4 enhances resistance to sclerotinia sclerotiorum in oilseed rape. Mol Plant-Microbe Interact, 2009, 22: 235–244



[33]Mishra N S, Tuteja R, Tuteja N. Signaling through MAP kinase networks in plants. Arch Biochem Bioph, 2006, 452: 55–68



[34]Nakagami H, Pitzschke A, Hirt H. Emerging MAP kinase pathways in plant stress signalling. Trends Plant Sci, 2005, 10: 339–346



[34]Zhang T, Liu Y, Yang T, Zhang L, Xu S, Xue L, An L. Diverse signals converge at MAPK cascades in plant. Plant Physiol Biochem, 2006, 44: 274–283



[36]Brodersen P, Petersen M, Nielsen H B, Zhu S J, Newman M A, Shokat K M, Rietz S, Parker J, Mundy J. Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4. Plant J, 2006, 47: 532–546



[37]Kovtun Y, Chiu W L, Tena G, Sheen J. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proc Nat Acad Sci USA, 2000, 97: 2940–2945



[38]Rentel M C, Lecourieux D, Ouaked F, Usher S L, Petersen L, Okamoto H, Knight H, Peck S C, Grierson C S, Hirt H, Knight M R. OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis. Nature, 2004, 427: 858–861



[39]Yuasa Y, Ichimura K, Mizoguchi T, Shinozaki K. Oxidative stress activates ATMPK6, an Arabidopsis homologue of MAP kinase. Plant Cell Physiol, 2001, 42: 1012–1016



[40]Lu C, Han M H, Guevara-Garcia A, Fedoroff N V. Mitogen-activated protein kinase signaling in postgermination arrest of development by abscisic acid. Proc Nat Acad Sci USA, 2002, 99: 15812–15817



[41]Pedro A Q, Bonifacio M J, Queiroz J A, Maia C J, Passarinha L A. A novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-O-methyltransferase. J Biotechnol, 2011, 156: 141–146



[42]Liu B Q, Li G X, Sui X W, Yin J, Wang H, Ren X F. Expression and functional analysis of porcine aminopeptidase N produced in prokaryotic expression system. J Biotechnol, 2009, 141: 91–96

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