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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (08): 1409-1415.doi: 10.3724/SP.J.1006.2013.01409

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Gene Mapping of a Rice White Midrib Mutant wpsm

ZHU Xiao-Yan,XU Fang-Fang,SANG Xian-Chun,JIANG Yu-Dong,WANG Nan,ZHANG Chang-Wei,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716, China
  • Received:2012-11-15 Revised:2013-03-22 Online:2013-08-12 Published:2013-05-22
  • Contact: 何光华,E-mail: hegh@swu.edu.cn

Abstract:

A white midrib mutant temparily designated as wpsm (white primary and secondary midrib) was screened out in the progeny of an excellent indica restorer line Jinhui 10 with seeds treated by ethyl methane sulfonate (EMS). The wpsm mutant displayed normal leaves during the seedling stage. In the late booting stage, the primary and secondary midrib of the first, second and third leaves of the wpsm mutant showed a bleached appearance, compared with those of the wild type, while mesophyll cells kept normal. This mutational character was maintained to maturity. The photosynthetic pigment of the wpsm was significantly lower than that of the wild type, so were Pn and ETR. Agronomic traits such as plant height, filled grain number per panicle, seed setting rate and 1000-grain weight were significantly decreased when compared with the wild type. Genetic analysis suggested that the mutational characters were controlled by a single recessive nuclear gene. Xinong 1A was crossed with the wpsm, 892 recessive mutants from the F2 segregation population were used for gene mapping. WPSM was mapped on chromosome 6, between InDel 10 and InDel 4, with physical distance of about 56 kb. This result provides a foundation for WPSM gene cloning by map-based strategy as well as its functional analysis.

Key words: Oryza sativa L., Midrib, White, Gene mapping

[1]Rudiger W. Chlorophyll metabolism: from outer space down to the molecular level. Phytochemistry, 1997, 46: 1151–1167



[2]Bollivar D W. Recent advances in chlorophyll biosynthesis. Photosynth Res, 2006, 90: 173–194



[3]Kurata N, Miyoshi K, Nonomura K I, Yamazaki Y, Ito Y. Rice mutants and genes related to organ development, morphogenesis and physiological traits. Plant Cell Physiol, 2005, 46: 48–62



[4]Chen G, Bi Y R, Li N. EGY1 encodes a membrane-associated and ATP-independent metalloprotease that is required for chloroplast development. Plant J, 2005, 41: 364–375



[5]Larkin R M, Alonso J M, Ecker J R, Chory J. GUN4, a regulator of chlorophyll synthesis and intracellular signaling. Science, 2003, 299: 902–906



[6]Omura T, Iwata N, Satoh H. Linkage studies in rice (Oryza sativa L.) on some virescent and chlorine mutants, J Faculty Agric Kyshu Univ, 1978, 23: 85–93



[7]Kusumi K, Mizutani A, Nishimura M, Iba K. A virescent gene V1 determinates the expression timing of plastid genes for transcription/translation apparatus during early leaf development in rice. Plant J, 1997, 12: 1241–1250



[8]Kusumi K, Sakata C, Nakamura T, Kawasaki S, Yoshimura A, Iba K. A plastid protein NUS1 is essential for build-up the genetic system for early chloroplast development under cold stress conditions. Plant J, 2011, 68: 1039–1050



[9]Li H-C(李红昌), Qian Q(钱前), Wang B(王斌), Li X-B(李晓波), Zhu L-H(朱立煌), Xu J-C(徐吉臣). Identification and chromosomal localization of rice white panicle. Chin Sci Bull (科学通报), 2003, 48(3): 268–270 (in Chinese)



[10]Li N(李娜), Chu H-W(储黄伟), Wen T-Q(文铁桥), Zhang D-B(张大兵). Genetic analysis and mapping of the rice white midrib mutant Oswm. Acta Agric Shanghai (上海农业学报), 2007, 23(1): 1–4 (in Chinese with English abstract)



[11]Hu J-T(胡景涛), Zhang J(张甲), Li Y-Y(李园园), Fu C-Y(付崇允), Zheng J(郑静), Chen J-B(陈家彬), Hu Y(胡燕), Li S-G(李仕贵). Genetic analysis and mapping of a rice white midrib mutant Oswm2. Hereditas (遗传), 2008, 31(9): 1201–1206 (in Chinese with English abstract)



[12]Wellburn A R. The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol, 1994, 144: 307–313



[13]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832



[14]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325



[15]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLPs) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 252: 597–607



[16]Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174–181



[17]Kosambi D D. The estimation of map distances from recombination valurres. Ann Human Genet, 1944, 12: 172–175



[18]Jung K H, Hur J, Ryu C H, Chung Y Y, Miyao A, Hirochika H, Gynheung A. Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system. Plant Cell Physiol, 2003, 44: 463–472



[19]Zhang H T, Li J J, Yoo J H, Yoo S C, Cho S H, Koh H J, Seo H S, Paek N C. Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development. Plant Mol Biol, 2006, 62: 325–337



[20]Wu Z M, Zhang X, He B, Diao L P, Sheng S L, Wang J L, Guo X P, Sun N, Wang L F, Jiang L, Wang C W, Zhai H Q, Wan J M. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29–40



[21]Sichul L, Kim J H, Yoo E S, Lee C H, Hirohiko H, Gynhenng A. A differential regulation of chlorophyll a oxygenase genes in rice. Plant Mol Biol, 2005, 57: 805–818



[22]Kusumi K, Yara A, Mitsui N, Tozawa Y, Iba K. Characterization of a rice nuclear-encoded plastid RNA polymerase gene OsRpoTP. Plant Cell Physiol, 2004, 45: 1194–1201



[23]Jiang H, Liang N, Yan H, Wei Y, Xu X, Liu J, Xu Z, Chen F, Wu G. Molecular cloning and function analysis of the stay green gene in rice. Plant J, 2007, 52: 197–209



[24]Sugimoto H, Kusumi K, Noguchi K, Yano M, Yoshimura A, Iba K. The rice nuclear gene, VIRESCENT 2, is essential for chloroplast development and encodes a novel type of guanylate kinase targeted to plastids and mitochondria. Plant J, 2007, 52: 512–527



[25]Yoo S C, Cho S H, Sugimoto H, Li Jinjie, Kusumi K, Koh H J, Iba K, Paek N C. Rice virescent 3 and stripe 1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development. Plant Physiol, 2009, 150: 388–401



[26]Kusaba M, Ito H, Morita R, Iida S, Sato Y, Fujimoto M, Kawasaki S, Tanaka R, Hirochika H, Nishimura M, Tanaka A. Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence. Plant Cell, 2007, 19: 1362–1375



[27]Krall J P, Edward G E. Relationship between photosystem II activity and CO2 fixation in leaves. Physiol Plant, 1992, 86: 180–187



[28]Demmig B, Winter K, Kruger A, Czygan F C. Photoinhibition and zeaxanthin formation in intact leaves: a possible role of the xanthophyll cycle in the dissipation of excess light energy. Plant Physiol, 1987, 84: 218–224



[29]Gray G R, Chauvin L P, Sarhan F, Hunter NPA. Cold acclimation and freezing tolerance: a complex interaction of light and temperature. Plant Physiol, 1997, 114: 464–474



[30]Maxwell K, Johnson G N. Chlorophyll fluorescence-a practical guide. J Exp Bot, 2000, 51: 659–668



[31]Michal K. On the relation between the non-photochemical quenching of chlorophyll fluorescence and photosystem I light harvesting efficiency a repetitive flash fluorescence induction study. Photosynth Res, 2001, 68: 571–576



[32]Xu F-H(许凤华), Cheng Z-J(程治军), Wang J-L(王久林), Wu Z-M(吴自明), Sun W(孙伟), Zhang X(张欣), Lei C-L(雷财林), Wang J(王洁), Wu F-Q(吴赴涛), Guo X-P(郭秀平), Liu L-L(刘玲珑), Wan J-M(万建民). Genetic analysis and fine mapping of gws gene using green-white-stripe rice mutant. Acta Agron Sin (作物学报), 2010, 36(5): 713–720 (in Chinese with English abstract)



[33]Sang X-C(桑贤春), Xu F-F(徐芳芳), Ling Y-H(凌英华), Zhao F-M(赵芳明), Yang Z-L(杨正林), Tang Y-Q(唐彦强), Tian X-Q(田晓庆), Li Y-F(李云峰), He G-H(何光华). Identification and molecular mapping of stripe leaf mutant st(t) in rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2010, 36(2): 211–216 (in Chinese with English abstract)



[34]Song L-L(宋莉英), Sun L-L(孙兰兰), Shu Z(舒展), Zeng W(曾伟), Li W-H(李伟华), Peng C-L(彭长连). Effects of drought stress and rehydration on chlorophyll fluorescence characteristics in leaves of invasive Wedelia trilobata. Acta Ecol Sin (生态学报), 2009, 29(7): 3713–3721 (in Chinese with English abstract)



[35]Zhang S-R(张守仁). A discussion on chlorophyll fluorescence kinetics parameters and their significance. Chin Bull Bot (植物学通报), 1999, 16(4): 444–448 (in Chinese with English abstract)

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