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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (02): 369-379.doi: 10.3724/SP.J.1006.2014.00369

• RESEARCH NOTES • Previous Articles    

Genetic Diversity Analysis on Local Upland Cotton Cultivars in Xinjiang Based on SSR Markers

AI Xian-Tao1, 2,LIANG Ya-Jun2,SHA Hong2,WANG Jun-Duo2,ZHENG Ju-Yun2,Tu-Er-Xun-Jiang2,DUO Li-Kun2,LI Xue-Yuan2, *,HUA Jin-Ping3,*   

  1. 1Xinjiang Agricultural University , Urumqi 830052, China; 2 Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; 3 Department of Plant Genetics and Breeding / Key Laboratory of Crop Heterosis and Utilization of Ministry of Education / Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
  • Received:2013-01-21 Revised:2013-08-16 Online:2014-02-12 Published:2013-10-22
  • Contact: 华金平, E-mail: jinping_hua@cau.edu.cn; 李雪源, E-mail: xjmh2338@163.com

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Abstract:

Xinjiang, the largest prior cotton production region in China, has made remarkable genetic improvement in upland cotton (Gossypium hirsutum L.). There has been a substantial raise in yield potential, cotton-planting area and total production in Xinjiang since 1970s. The new upland cotton cultivars have contributed a lot to yield increase, in which Xinjiang-bred upland cotton varieties play an important role in cotton production in both Northern and Southern Xinjiang. However, seldom research on genetic basis of genetic diversity and pedigree relationship has been evaluated in upland cotton varieties in Xinjiang so far. This study aimed to reveal the genetic diversity of 94 upland cotton varieties in Xinjiang by genome analysis using SSR markers. The results indicated that SSR molecular markers could be employed in revealing the genetic diversity. One hundred and fifty-three polymorphic lociwere detected by amplified with 54 pairs of primers with allelic variation of 2-6 per primer pair and the average of 2.93. The genotypic diversity (H’) spanned from 0.0439 to 0.7149, with the average of 0.4491. The PIC value ranged from 0.0430 to 0.6640, and the average was 0.3831. The coefficients of genetic similarity among 94 tested cultivars varied from 0.3846 to 0.9835. And more than 71.9% of similarity coefficients fell into the interval of 0.601–0.800, indicating that the genetic similarity among the cultivars was high, whereas the genetic diversity might not be abundant. According to UPGMA cluster analysis, 94 tested cultivars were divided into two clusters when the threshold valve was given as 0.63, showing the relatively simple genetic relationship among the upland cotton varieties in Xinjiang, and relatively low genetic diversity and narrow genetic basis. The results of molecular clustering on genetic diversity of upland cotton coincided in large degree with the pedigree relationship. In conclusion, there is no much difference on molecular level among Xinjiang-bred upland cotton varieties. Materials with much wider genetic backgrounds are suggested to be used to diversify the genetic basis of cotton varieties in Xinjiang.

Key words: Upland cotton, Genetic background, SSR, Genetic diversity, Genetic relationship

[1]周盛汉. 中国棉花品种系谱图. 成都: 四川科学技术出版社, 2000



Zhou S H. China Cotton Varieties Pedigree Diagram. Sichuan Publishing House of Science & Technology, 2000 (in Chinese)



[2]董玉琛, 刘旭. 中国作物野生近缘植物及其保护. 见: 中国科学院生物多样性委员会等编. 生物多样性与人类未来. 北京: 中国林业出版社, 1998, pp 24–29



Dong Y C, Liu X. Wild relative crops and their protection in China. In: Biodiversity Committee, CAS, etc. eds. Biological Diversity and the Future of Human Beings, Beijing: Chinese Forestry Press 1998, pp 24–29 (in Chinese)



[3]朱四元, 陈金湘, 刘爱玉, 李瑞莲, 严跃文, 唐海明. 利用SSR标记对不同类型抗虫棉品种的遗传多样性分析. 湖南农业大学学报, 2006, 32: 469–472



Zhu S Y, Chen J X, Liu A Y, Li R L, Yan Y W, Tang H M. Genetic diversity analysis and identification of insect-resistant cottons based upon SSR markers. J Hunan Agric Univ (Nat Sci), 2006, 32: 469–472 (in Chinese with English abstract)



[4]武耀廷, 张天真, 殷剑美. 利用分子标记和形态学性状检测的陆地棉栽培品种遗传多样性. 遗传学报, 2001, 28: 1040–1050



Wu Y T, Zhang T Z, Yin J M. Genetic diversity detected by DNA markers and phenotypes in upland cotton. Acta Genet Sin, 2001, 28: 1040–1050 (in Chinese with English abstract)



[5]李武, 倪薇, 林忠旭, 张献龙. 海岛棉遗传多样性的SRAP标记分析. 作物学报, 2008, 34: 893–898



Li W, Ni W, Lin Z X, Zhang X L. Genetic diversity analysis of sea-island cotton cultivars using SRAP markers. Acta Agron Sin, 2008, 34(5): 893–898 (in Chinese with English abstract)



[6]郭旺珍,周兆华,张天真. RAPD鉴定棉花抗(耐)黄萎病品种(系)的遗传变异研究. 江苏农业学报,1999, 15(1): 1–6



Guo W Z, Zhou Z H, Zhang T Z. A study on cotton genetic variation of Verticillium wilt resistance based on RAPDs. J Jiangsu Agric, 1999, 15(1): 1–6 (in Chinese with English abstract)



[7]凌磊, 李廷春, 李正鹏. 利用SRAP标记分析彩色棉与白色棉的遗传差异. 中国农学通报, 2009, 25(16): 32–38



Ling L, Li T C, Li Z P. Genetic difference analysis between colored cotton and white cotton by SRAP markers. Chin Agric Sci Bull, 2009, 25(16): 32–38 (in Chinese with English abstract)



[8]卫泽, 孙学振, 宋宪亮. 国内外57份棉花种质资源的遗传多样性研究. 山东农业科学, 2010, 6: 13–18



Wei Z, Sun X Z, Song X L. Genetic diversity of 57 cotton germplasm resources from China and overseas. Shandong Agric Sci, 2010, (6): 13–18 (in Chinese with English abstract)



[9]徐秋华, 张献龙, 聂以春. 长江、黄河两棉区陆地棉品种遗传多样性比较研究. 遗传学报, 2001, 8: 683–690



Xu Q H, Zhang X L, Nie Y C. Genetic diversity evaluation of cultivars (L.) from the Chang Jiang River Valley and the Huanghe Valley by RAPD. Acta Genet Sin, 2001, 28: 683–690 (in Chinese with English abstract)



[10]刘文欣, 孔繁玲, 郭志丽, 张群远, 彭慧茹, 付小琼, 杨付新. 建国以来我国棉花品种遗传基础的分子标记分析. 遗传学报, 2003, 30: 560–570



Liu W X, Kong F L, Guo Z L, Zhang Q Y, Peng H R, Fu X Q, Yang F X. Analysis about genetic basis of cotton cultivars in China since 1949 with molecular markers. Acta Genet Sin, 2003, 30: 560–570 (in Chinese with English abstract)



[11]宋国立, 崔荣霞, 王坤波, 郭立平, 黎绍惠, 王春英, 张香娣. 改良CTAB法快速提取棉花DNA. 棉花学报, 1998, 10(5): 273–275



Song G L, Cui R X, Wang K B, Guo L P, Li S H, Wang C Y, Zhang X D. The improved CTAB law rapid extraction of genomic DNA from cotton. Acta Gossypii Sin, 1998, 10: 273–275 (in Chinese with English abstract)



[12]Xiao J, Wu K, Fang D D, Stelly D M, Yu J, Cantrell R G. New SSR markers for Use in cotton (Gossypium spp.) improvement. J Cotton Sci, 2009, 13: 75–157



[13]艾先涛, 李雪源, 秦文斌, 孙国清, 吐尔逊江, 莫明. 新疆陆地棉育种遗传组分拓展研究. 分子植物育种, 2005, 3: 575–578



Ai X T, Li X Y, Qin W B, Sun G Q, Tu E X J, Mo M. The extend study on genetic composition of upland cotton breeding in Xinjiang. Mol Plant Breed, 2005, 3: 575–578 (in Chinese with English abstract)



[14]贺道华, 邢宏宜, 李婷婷, 汤益, 曾舟. 92份棉花资源遗传多样性的SSR分析. 西北植物学报, 2010, 30: 1557–1564



He D H, Xing H Y, Li T T, Tang Y, Zeng Z. Genetic diversity of 92 cotton accessions evaluated with SSR markers. Northwest Agric Univ, 2010, 30: 1557–1564 (in Chinese with English abstract)



[15]高伟, 刘方, 黎绍惠, 王春英, 张香娣, 王玉红, 王坤波. 利用SSR分析四倍体棉种多态性.作物学报, 2010, 36: 1902–1909



Gao W, Liu F, Li S H, Wang C Y, Zhang X D, Wang Y H, Wang K B. Genetic diversity of allotetraploid cotton based on SSR markers. Acta Agron Sin, 2010, 36: 1902−1909 (in Chinese with English abstract)



[16]别墅, 孔繁玲, 周有耀, 张光梅, 张群远, 王孝纲. 中国三大主产棉区棉花品种遗传多样性的RAPD及其与农艺性状关系的研究. 中国农业科学, 2001, 34: 597–603



Bie S, Kong F L, Zhou Y Y, Zhang G M, Zhang Q Y, Wang X G. Genetic diversity analysis representative elite cotton varieties in three main cotton regions in China by RAPD and its relation with agronomic characteristics. Sci Agric Sin, 2001, 34: 597–603 (in Chinese with English abstract)



[17]陈光. 我国陆地棉基础种质及其衍生品种的遗传多样性. 中国农业科学院研究生院硕士学位论文, 2005



Chen G. Genetic Diversity of Basic Germplasm and Their Offsprings of Upland Cotton in China. MS Thesis of Graduate School of Chinese Academy of Agricultural Sciences, 2005 (in Chinese with English abstract)



[18]杜雄明, 刘国强. 论我国棉花育种的基础种质. 植物遗传资源学报. 2004, 5: 69–74



Du X M, Liu G Q. Basic Germplasm for Cotton Breeding in China. J Plant Genet Resour, 2004, 5: 69–74 (in Chinese with English abstract)



[19]薛艳, 张新宇, 沙红, 李雪源, 孙杰, 李保成. 新疆早熟棉品种SSR指纹图谱构建与品种鉴别. 棉花学报, 2010, 22: 360–366



Xue Y, Zhang X Y, Sha H, Li X Y, Sun J, Li B C. Construction of Fingerprinting Map Based on SSR and Identification of Cultivars for Earliness Cultivars in Upland Cotton in Xinjiang. Acta Gossypii Sin, 2010, 22: 360–366 (in Chinese with English abstract)



[20]黄滋康, 季道藩. 中国棉花遗传育种学. 济南: 山东科学技术出版社, 2003



Hang Z K, Ji D F. China Cotton Genetics and Breeding. Jinan: Shandong Science & Technology Press, 2003



[21]孙杰, 褚贵新, 张文辉, 金文虹. 新疆特早熟棉区棉花品种主要性状演变趋势研究. 中国棉花, 1999, 26(7): 14–16



Sun J, Zhe G X, Zhang W H, Jin W H. The main character trend of especially early maturing varieties of cotton in Xinjiang. China’s Cotton, 1999, 26(7): 14–16 (in Chinese with English abstract)



[22]黄滋康. 中国棉花品种及其系谱. 北京: 中国农业出版社, 2007



Hang Z K. China's cotton varieties and its pedigree. Beijing: China Agricultural Press, 2007



[23]Yu F F, Huaxia Y F, Lu W J, Wu C G, Cao X C, Guo X Q. GhWRKY15, a member of the WRKY transcription factor family identified from cotton (Gossypium hirsutum L.), is involved in disease resistance and plant development, BMC Plant Biol, 2012, 12: 144



[24]Zhang Y, Wang X F, Li Z K, Zhang G Y. Assessing genetic diversity of cotton cultivars using genomic and newly developed expressed sequence tag-derived microsatellite markers. Genet Mol Res, 2011, 10: 1462–1470
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